Metabotropic Glutamate Receptor 2 (GRM2) (NM_000839) Human Tagged ORF Clone

CAT#: RC218103

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GRM2 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1



  "NM_000839" in other vectors (6)

Reconstitution Protocol

USD 798.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GRM2
Synonyms GLUR2; GPRC1B; mGlu2; MGLUR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218103 representing NM_000839
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGATCGCTGCTTGCGCTCCTGGCACTGCTGCTGCTGTGGGGTGCTGTGGCTGAGGGCCCAGCCAAGA
AGGTGCTGACCCTGGAGGGAGACTTGGTGCTGGGTGGGCTGTTCCCAGTGCACCAGAAGGGCGGCCCAGC
AGAGGACTGTGGTCCTGTCAATGAGCACCGTGGCATCCAGCGCCTGGAGGCCATGCTTTTTGCACTGGAC
CGCATCAACCGTGACCCGCACCTGCTGCCTGGCGTGCGCCTGGGTGCACACATCCTCGACAGTTGCTCCA
AGGACACACATGCGCTGGAGCAGGCACTGGACTTTGTGCGTGCCTCACTCAGCCGTGGTGCTGATGGCTC
ACGCCACATCTGCCCCGACGGCTCTTATGCGACCCATGGTGATGCTCCCACTGCCATCACTGGTGTTATT
GGCGGTTCCTACAGTGATGTCTCCATCCAGGTGGCCAACCTCTTGAGGCTATTTCAGATCCCACAGATTA
GCTACGCCTCTACCAGTGCCAAGCTGAGTGACAAGTCCCGCTATGACTACTTTGCCCGCACAGTGCCTCC
TGACTTCTTCCAAGCCAAGGCCATGGCTGAGATTCTCCGCTTCTTCAACTGGACCTATGTGTCCACTGTG
GCGTCTGAGGGCGACTATGGCGAGACAGGCATTGAGGCCTTTGAGCTAGAGGCTCGTGCCCGCAACATCT
GTGTGGCCACCTCGGAGAAAGTGGGCCGTGCCATGAGCCGCGCGGCCTTTGAGGGTGTGGTGCGAGCCCT
GCTGCAGAAGCCCAGTGCCCGCGTGGCTGTCCTGTTCACCCGTTCTGAGGATGCCCGGGAGCTGCTTGCT
GCCAGCCAGCGCCTCAATGCCAGCTTCACCTGGGTGGCCAGTGATGGTTGGGGGGCCCTGGAGAGTGTGG
TGGCAGGCAGTGAGGGGGCTGCTGAGGGTGCTATCACCATCGAGCTGGCCTCCTACCCCATCAGTGACTT
TGCCTCCTACTTCCAGAGCCTGGACCCTTGGAACAACAGCCGGAACCCCTGGTTCCGTGAATTCTGGGAG
CAGAGGTTCCGCTGCAGCTTCCGGCAGCGAGACTGCGCAGCCCACTCTCTCCGGGCTGTGCCCTTTGAGC
AGGAGTCCAAGATCATGTTTGTGGTCAATGCAGTGTACGCCATGGCCCATGCGCTCCACAACATGCACCG
TGCCCTCTGCCCCAACACCACCCGGCTCTGTGACGCGATGCGGCCAGTTAACGGGCGCCGCCTCTACAAG
GACTTTGTGCTCAACGTCAAGTTTGATGCCCCCTTTCGCCCAGCTGACACCCACAATGAGGTCCGCTTTG
ACCGCTTTGGTGATGGTATTGGCCGCTACAACATCTTCACCTATCTGCGTGCAGGCAGTGGGCGCTATCG
CTACCAGAAGGTGGGCTACTGGGCAGAAGGCTTGACTCTGGACACCAGCCTCATCCCATGGGCCTCACCC
TCAGCCGGCCCCCTGCCCGCCTCTCGCTGCAGTGAGCCCTGCCTCCAGAATGAGGTGAAGAGTGTGCAGC
CGGGCGAAGTCTGCTGCTGGCTCTGCATTCCGTGCCAGCCCTATGAGTACCGATTGGACGAATTCACTTG
CGCTGATTGTGGCCTGGGCTACTGGCCCAATGCCAGCCTGACTGGCTGCTTCGAACTGCCCCAGGAGTAC
ATCCGCTGGGGCGATGCCTGGGCTGTGGGACCTGTCACCATCGCCTGCCTCGGTGCCCTGGCCACCCTCT
TTGTGCTGGGTGTCTTTGTGCGGCACAATGCCACACCAGTGGTCAAGGCCTCAGGTCGGGAGCTCTGCTA
CATCCTGCTGGGTGGTGTCTTCCTCTGCTACTGCATGACCTTCATCTTCATTGCCAAGCCATCCACGGCA
GTGTGTACCTTACGGCGTCTTGGTTTGGGCACTGCCTTCTCTGTCTGCTACTCAGCCCTGCTCACCAAGA
CCAACCGCATTGCACGCATCTTCGGTGGGGCCCGGGAGGGTGCCCAGCGGCCACGCTTCATCAGTCCTGC
CTCACAGGTGGCCATCTGCCTGGCACTTATCTCGGGCCAGCTGCTCATCGTGGTCGCCTGGCTGGTGGTG
GAGGCACCGGGCACAGGCAAGGAGACAGCCCCCGAACGGCGGGAGGTGGTGACACTGCGCTGCAACCACC
GCGATGCAAGTATGTTGGGCTCGCTGGCCTACAATGTGCTCCTCATCGCGCTCTGCACGCTTTATGCCTT
CAAGACTCGCAAGTGCCCCGAAAACTTCAACGAGGCCAAGTTCATTGGCTTCACCATGTACACCACCTGC
ATCATCTGGCTGGCATTCCTGCCCATCTTCTATGTCACCTCCAGTGACTACCGGGTACAGACCACCACCA
TGTGCGTGTCAGTCAGCCTCAGCGGCTCCGTGGTGCTTGGCTGCCTCTTTGCGCCCAAGCTGCACATCAT
CCTCTTCCAGCCGCAGAAGAACGTGGTTAGCCACCGGGCACCCACCAGCCGCTTTGGCAGTGCTGCTGCC
AGGGCCAGCTCCAGCCTTGGCCAAGGGTCTGGCTCCCAGTTTGTCCCCACTGTTTGCAATGGCCGTGAGG
TGGTGGACTCGACAACGTCATCGCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218103 representing NM_000839
Red=Cloning site Green=Tags(s)

MGSLLALLALLLLWGAVAEGPAKKVLTLEGDLVLGGLFPVHQKGGPAEDCGPVNEHRGIQRLEAMLFALD
RINRDPHLLPGVRLGAHILDSCSKDTHALEQALDFVRASLSRGADGSRHICPDGSYATHGDAPTAITGVI
GGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFFQAKAMAEILRFFNWTYVSTV
ASEGDYGETGIEAFELEARARNICVATSEKVGRAMSRAAFEGVVRALLQKPSARVAVLFTRSEDARELLA
ASQRLNASFTWVASDGWGALESVVAGSEGAAEGAITIELASYPISDFASYFQSLDPWNNSRNPWFREFWE
QRFRCSFRQRDCAAHSLRAVPFEQESKIMFVVNAVYAMAHALHNMHRALCPNTTRLCDAMRPVNGRRLYK
DFVLNVKFDAPFRPADTHNEVRFDRFGDGIGRYNIFTYLRAGSGRYRYQKVGYWAEGLTLDTSLIPWASP
SAGPLPASRCSEPCLQNEVKSVQPGEVCCWLCIPCQPYEYRLDEFTCADCGLGYWPNASLTGCFELPQEY
IRWGDAWAVGPVTIACLGALATLFVLGVFVRHNATPVVKASGRELCYILLGGVFLCYCMTFIFIAKPSTA
VCTLRRLGLGTAFSVCYSALLTKTNRIARIFGGAREGAQRPRFISPASQVAICLALISGQLLIVVAWLVV
EAPGTGKETAPERREVVTLRCNHRDASMLGSLAYNVLLIALCTLYAFKTRKCPENFNEAKFIGFTMYTTC
IIWLAFLPIFYVTSSDYRVQTTTMCVSVSLSGSVVLGCLFAPKLHIILFQPQKNVVSHRAPTSRFGSAAA
RASSSLGQGSGSQFVPTVCNGREVVDSTTSSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000839
ORF Size 2616 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000839.5
RefSeq Size 3186 bp
RefSeq ORF 2619 bp
Locus ID 2912
UniProt ID Q14416
Cytogenetics 3p21.2
Domains 7tm_3, ANF_receptor
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 95.57 kDa
Gene Summary L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017]

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