Metabotropic Glutamate Receptor 2 (GRM2) (NM_000839) Human Tagged ORF Clone

CAT#: RC218103

GRM2 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY424482) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218103 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Metabotropic Glutamate Receptor 2
• Synonyms: GLUR2; GPRC1B; mGlu2; MGLUR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218103 representing NM_000839
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATCGCTGCTTGCGCTCCTGGCACTGCTGCTGCTGTGGGGTGCTGTGGCTGAGGGCCCAGCCAAGA
  AGGTGCTGACCCTGGAGGGAGACTTGGTGCTGGGTGGGCTGTTCCCAGTGCACCAGAAGGGCGGCCCAGC
  AGAGGACTGTGGTCCTGTCAATGAGCACCGTGGCATCCAGCGCCTGGAGGCCATGCTTTTTGCACTGGAC
  CGCATCAACCGTGACCCGCACCTGCTGCCTGGCGTGCGCCTGGGTGCACACATCCTCGACAGTTGCTCCA
  AGGACACACATGCGCTGGAGCAGGCACTGGACTTTGTGCGTGCCTCACTCAGCCGTGGTGCTGATGGCTC
  ACGCCACATCTGCCCCGACGGCTCTTATGCGACCCATGGTGATGCTCCCACTGCCATCACTGGTGTTATT
  GGCGGTTCCTACAGTGATGTCTCCATCCAGGTGGCCAACCTCTTGAGGCTATTTCAGATCCCACAGATTA
  GCTACGCCTCTACCAGTGCCAAGCTGAGTGACAAGTCCCGCTATGACTACTTTGCCCGCACAGTGCCTCC
  TGACTTCTTCCAAGCCAAGGCCATGGCTGAGATTCTCCGCTTCTTCAACTGGACCTATGTGTCCACTGTG
  GCGTCTGAGGGCGACTATGGCGAGACAGGCATTGAGGCCTTTGAGCTAGAGGCTCGTGCCCGCAACATCT
  GTGTGGCCACCTCGGAGAAAGTGGGCCGTGCCATGAGCCGCGCGGCCTTTGAGGGTGTGGTGCGAGCCCT
  GCTGCAGAAGCCCAGTGCCCGCGTGGCTGTCCTGTTCACCCGTTCTGAGGATGCCCGGGAGCTGCTTGCT
  GCCAGCCAGCGCCTCAATGCCAGCTTCACCTGGGTGGCCAGTGATGGTTGGGGGGCCCTGGAGAGTGTGG
  TGGCAGGCAGTGAGGGGGCTGCTGAGGGTGCTATCACCATCGAGCTGGCCTCCTACCCCATCAGTGACTT
  TGCCTCCTACTTCCAGAGCCTGGACCCTTGGAACAACAGCCGGAACCCCTGGTTCCGTGAATTCTGGGAG
  CAGAGGTTCCGCTGCAGCTTCCGGCAGCGAGACTGCGCAGCCCACTCTCTCCGGGCTGTGCCCTTTGAGC
  AGGAGTCCAAGATCATGTTTGTGGTCAATGCAGTGTACGCCATGGCCCATGCGCTCCACAACATGCACCG
  TGCCCTCTGCCCCAACACCACCCGGCTCTGTGACGCGATGCGGCCAGTTAACGGGCGCCGCCTCTACAAG
  GACTTTGTGCTCAACGTCAAGTTTGATGCCCCCTTTCGCCCAGCTGACACCCACAATGAGGTCCGCTTTG
  ACCGCTTTGGTGATGGTATTGGCCGCTACAACATCTTCACCTATCTGCGTGCAGGCAGTGGGCGCTATCG
  CTACCAGAAGGTGGGCTACTGGGCAGAAGGCTTGACTCTGGACACCAGCCTCATCCCATGGGCCTCACCC
  TCAGCCGGCCCCCTGCCCGCCTCTCGCTGCAGTGAGCCCTGCCTCCAGAATGAGGTGAAGAGTGTGCAGC
  CGGGCGAAGTCTGCTGCTGGCTCTGCATTCCGTGCCAGCCCTATGAGTACCGATTGGACGAATTCACTTG
  CGCTGATTGTGGCCTGGGCTACTGGCCCAATGCCAGCCTGACTGGCTGCTTCGAACTGCCCCAGGAGTAC
  ATCCGCTGGGGCGATGCCTGGGCTGTGGGACCTGTCACCATCGCCTGCCTCGGTGCCCTGGCCACCCTCT
  TTGTGCTGGGTGTCTTTGTGCGGCACAATGCCACACCAGTGGTCAAGGCCTCAGGTCGGGAGCTCTGCTA
  CATCCTGCTGGGTGGTGTCTTCCTCTGCTACTGCATGACCTTCATCTTCATTGCCAAGCCATCCACGGCA
  GTGTGTACCTTACGGCGTCTTGGTTTGGGCACTGCCTTCTCTGTCTGCTACTCAGCCCTGCTCACCAAGA
  CCAACCGCATTGCACGCATCTTCGGTGGGGCCCGGGAGGGTGCCCAGCGGCCACGCTTCATCAGTCCTGC
  CTCACAGGTGGCCATCTGCCTGGCACTTATCTCGGGCCAGCTGCTCATCGTGGTCGCCTGGCTGGTGGTG
  GAGGCACCGGGCACAGGCAAGGAGACAGCCCCCGAACGGCGGGAGGTGGTGACACTGCGCTGCAACCACC
  GCGATGCAAGTATGTTGGGCTCGCTGGCCTACAATGTGCTCCTCATCGCGCTCTGCACGCTTTATGCCTT
  CAAGACTCGCAAGTGCCCCGAAAACTTCAACGAGGCCAAGTTCATTGGCTTCACCATGTACACCACCTGC
  ATCATCTGGCTGGCATTCCTGCCCATCTTCTATGTCACCTCCAGTGACTACCGGGTACAGACCACCACCA
  TGTGCGTGTCAGTCAGCCTCAGCGGCTCCGTGGTGCTTGGCTGCCTCTTTGCGCCCAAGCTGCACATCAT
  CCTCTTCCAGCCGCAGAAGAACGTGGTTAGCCACCGGGCACCCACCAGCCGCTTTGGCAGTGCTGCTGCC
  AGGGCCAGCTCCAGCCTTGGCCAAGGGTCTGGCTCCCAGTTTGTCCCCACTGTTTGCAATGGCCGTGAGG
  TGGTGGACTCGACAACGTCATCGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218103 representing NM_000839
  Red=Cloning site Green=Tags(s)
  
  MGSLLALLALLLLWGAVAEGPAKKVLTLEGDLVLGGLFPVHQKGGPAEDCGPVNEHRGIQRLEAMLFALD
  RINRDPHLLPGVRLGAHILDSCSKDTHALEQALDFVRASLSRGADGSRHICPDGSYATHGDAPTAITGVI
  GGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFFQAKAMAEILRFFNWTYVSTV
  ASEGDYGETGIEAFELEARARNICVATSEKVGRAMSRAAFEGVVRALLQKPSARVAVLFTRSEDARELLA
  ASQRLNASFTWVASDGWGALESVVAGSEGAAEGAITIELASYPISDFASYFQSLDPWNNSRNPWFREFWE
  QRFRCSFRQRDCAAHSLRAVPFEQESKIMFVVNAVYAMAHALHNMHRALCPNTTRLCDAMRPVNGRRLYK
  DFVLNVKFDAPFRPADTHNEVRFDRFGDGIGRYNIFTYLRAGSGRYRYQKVGYWAEGLTLDTSLIPWASP
  SAGPLPASRCSEPCLQNEVKSVQPGEVCCWLCIPCQPYEYRLDEFTCADCGLGYWPNASLTGCFELPQEY
  IRWGDAWAVGPVTIACLGALATLFVLGVFVRHNATPVVKASGRELCYILLGGVFLCYCMTFIFIAKPSTA
  VCTLRRLGLGTAFSVCYSALLTKTNRIARIFGGAREGAQRPRFISPASQVAICLALISGQLLIVVAWLVV
  EAPGTGKETAPERREVVTLRCNHRDASMLGSLAYNVLLIALCTLYAFKTRKCPENFNEAKFIGFTMYTTC
  IIWLAFLPIFYVTSSDYRVQTTTMCVSVSLSGSVVLGCLFAPKLHIILFQPQKNVVSHRAPTSRFGSAAA
  RASSSLGQGSGSQFVPTVCNGREVVDSTTSSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000839
• ORF Size: 2616 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000839.5
• RefSeq Size: 3186 bp
• RefSeq ORF: 2619 bp
• Locus ID: 2912
• UniProt ID: Q14416
• Cytogenetics: 3p21.2
• Domains: 7tm_3, ANF_receptor
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 95.57 kDa
• Gene Summary: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017]