Argonaute 2 (AGO2) (NM_012154) Human Tagged ORF Clone

CAT#: RC218078

AGO2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1

Product Images

Circular map for RC218078

• 

  Western blot validation of overexpression lysate (Cat# LY415922) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218078 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Argonaute 2
• Synonyms: CASC7; EIF2C2; LESKRES; LINC00980; PPD; Q10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218078 representing NM_012154
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACTCGGGAGCCGGCCCCGCACTTGCACCTCCTGCGCCGCCGCCCCCCATCCAAGGATATGCCTTCA
  AGCCTCCACCTAGACCCGACTTTGGGACCTCCGGGAGAACAATCAAATTACAGGCCAATTTCTTCGAAAT
  GGACATCCCCAAAATTGACATCTATCATTATGAATTGGATATCAAGCCAGAGAAGTGCCCGAGGAGAGTT
  AACAGGGAAATCGTGGAACACATGGTCCAGCACTTTAAAACACAGATCTTTGGGGATCGGAAGCCCGTGT
  TTGACGGCAGGAAGAATCTATACACAGCCATGCCCCTTCCGATTGGGAGGGACAAGGTGGAGCTGGAGGT
  CACGCTGCCAGGAGAAGGCAAGGATCGCATCTTCAAGGTGTCCATCAAGTGGGTGTCCTGCGTGAGCTTG
  CAGGCGTTACACGATGCACTTTCAGGGCGGCTGCCCAGCGTCCCTTTTGAGACGATCCAGGCCCTGGACG
  TGGTCATGAGGCACTTGCCATCCATGAGGTACACCCCCGTGGGCCGCTCCTTCTTCACCGCGTCCGAAGG
  CTGCTCTAACCCTCTTGGCGGGGGCCGAGAAGTGTGGTTTGGCTTCCATCAGTCCGTCCGGCCTTCTCTC
  TGGAAAATGATGCTGAATATTGATGTGTCAGCAACAGCGTTTTACAAGGCACAGCCAGTAATCGAGTTTG
  TTTGTGAAGTTTTGGATTTTAAAAGTATTGAAGAACAACAAAAACCTCTGACAGATTCCCAAAGGGTAAA
  GTTTACCAAAGAAATTAAAGGTCTAAAGGTGGAGATAACGCACTGTGGGCAGATGAAGAGGAAGTACCGC
  GTCTGCAATGTGACCCGGCGGCCCGCCAGTCACCAAACATTCCCGCTGCAGCAGGAGAGCGGGCAGACGG
  TGGAGTGCACGGTGGCCCAGTATTTCAAGGACAGGCACAAGTTGGTTCTGCGCTACCCCCACCTCCCATG
  TTTACAAGTCGGACAGGAGCAGAAACACACCTACCTTCCCCTGGAGGTCTGTAACATTGTGGCAGGACAA
  AGATGTATTAAAAAATTAACGGACAATCAGACCTCAACCATGATCAGAGCGACTGCTAGGTCGGCGCCCG
  ATCGGCAAGAAGAGATTAGCAAATTGATGCGAAGTGCAAGTTTCAACACAGATCCATACGTCCGTGAATT
  TGGAATCATGGTCAAAGATGAGATGACAGACGTGACTGGGCGGGTGCTGCAGCCGCCCTCCATCCTCTAC
  GGGGGCAGGAATAAAGCTATTGCGACCCCTGTCCAGGGCGTCTGGGACATGCGGAACAAGCAGTTCCACA
  CGGGCATCGAGATCAAGGTGTGGGCCATTGCGTGCTTCGCCCCCCAGCGCCAGTGCACGGAAGTCCATCT
  GAAGTCCTTCACAGAGCAGCTCAGAAAGATCTCGAGAGACGCCGGCATGCCCATCCAGGGCCAGCCGTGC
  TTCTGCAAATACGCGCAGGGGGCGGACAGCGTGGAGCCCATGTTCCGGCACCTGAAGAACACGTATGCGG
  GCCTGCAGCTGGTGGTGGTCATCCTGCCCGGCAAGACGCCCGTGTACGCCGAGGTCAAGCGCGTGGGAGA
  CACGGTGCTGGGGATGGCCACGCAGTGCGTGCAGATGAAGAACGTGCAGAGGACCACGCCACAGACCCTG
  TCCAACCTCTGCCTGAAGATCAACGTCAAGCTGGGAGGCGTGAACAACATCCTGCTGCCCCAGGGCAGGC
  CGCCGGTGTTCCAGCAGCCCGTCATCTTTCTGGGAGCAGACGTCACTCACCCCCCCGCCGGGGATGGGAA
  GAAGCCCTCCATTGCCGCCGTGGTGGGCAGCATGGACGCCCACCCCAATCGCTACTGCGCCACCGTGCGT
  GTGCAGCAGCACCGGCAGGAGATCATACAAGACCTGGCCGCCATGGTCCGCGAGCTCCTCATCCAGTTCT
  ACAAGTCCACGCGCTTCAAGCCCACCCGCATCATCTTCTACCGCGACGGTGTCTCTGAAGGCCAGTTCCA
  GCAGGTTCTCCACCACGAGTTGCTGGCCATCCGTGAGGCCTGTATCAAGCTAGAAAAAGACTACCAGCCC
  GGGATCACCTTCATCGTGGTGCAGAAGAGGCACCACACCCGGCTCTTCTGCACTGACAAGAACGAGCGGG
  TTGGGAAAAGTGGAAACATTCCAGCAGGCACGACTGTGGACACGAAAATCACCCACCCCACCGAGTTCGA
  CTTCTACCTGTGTAGTCACGCTGGCATCCAGGGGACAAGCAGGCCTTCGCACTATCACGTCCTCTGGGAC
  GACAATCGTTTCTCCTCTGATGAGCTGCAGATCCTAACCTACCAGCTGTGTCACACCTACGTGCGCTGCA
  CACGCTCCGTGTCCATCCCAGCGCCAGCATACTACGCTCACCTGGTGGCCTTCCGGGCCAGGTACCACCT
  GGTGGATAAGGAACATGACAGTGCTGAAGGAAGCCATACCTCTGGGCAGAGTAACGGGCGAGACCACCAA
  GCACTGGCCAAGGCGGTCCAGGTTCACCAAGACACTCTGCGCACCATGTACTTTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218078 representing NM_012154
  Red=Cloning site Green=Tags(s)
  
  MYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRV
  NREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSL
  QALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSL
  WKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYR
  VCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQ
  RCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILY
  GGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPC
  FCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTL
  SNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVR
  VQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQP
  GITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWD
  DNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQ
  ALAKAVQVHQDTLRTMYFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012154
• ORF Size: 2577 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_012154.5
• RefSeq Size: 3567 bp
• RefSeq ORF: 2580 bp
• Locus ID: 27161
• UniProt ID: Q9UKV8
• Cytogenetics: 8q24.3
• Domains: PAZ, Piwi
• Protein Families: Druggable Genome
• MW: 97 kDa
• Gene Summary: This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]