C19orf2 (URI1) (NM_003796) Human Tagged ORF Clone

CAT#: RC217842

URI1 (Myc-DDK-tagged)-Human chromosome 19 open reading frame 2 (C19orf2), transcript variant 1

Product Images

Circular map for RC217842

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  Western blot validation of overexpression lysate (Cat# LY418431) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217842 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified URI1 protein (Cat# TP317842). The protein was produced from HEK293T cells transfected with URI1 cDNA clone (Cat# RC217842) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C19orf2
• Synonyms: C19orf2; NNX3; PPP1R19; RMP; URI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217842 representing NM_003796
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCCCACCGTGGAGACGCCCCCCGACCCCTCGCCCCCTTCGGCCCCGGCCCCTGCCCTGGTTC
  CGTTGCGCGCCCCGGATGTGGCGCGGCTGCGCGAGGAGCAGGAAAAGGTGGTCACTAACTGCCAAGAGAG
  AATCCAGCATTGGAAGAAGGTAGATAATGACTATAATGCCCTTCGAGAAAGACTCAGCACCTTGCCTGAT
  AAATTGTCTTATAATATAATGGTACCATTTGGCCCTTTTGCCTTCATGCCAGGAAAACTTGTCCATACTA
  ATGAAGTCACTGTTTTACTGGGGGACAACTGGTTTGCAAAGTGCTCAGCAAAGCAGGCTGTAGGTTTAGT
  TGAGCACCGGAAAGAACATGTAAGAAAAACAATAGATGACTTAAAAAAAGTGATGAAAAATTTTGAATCC
  AGAGTTGAATTCACAGAAGATTTGCAGAAAATGAGCGATGCTGCAGGTGATATTGTTGACATACGAGAAG
  AAATTAAATGTGACTTCGAATTTAAAGCAAAACACCGAATTGCTCATAAACCGCATTCCAAACCAAAAAC
  TTCAGATATTTTTGAAGCAGATATTGCAAATGATGTGAAATCCAAGGATTTGCTAGCTGATAAAGAACTG
  TGGGCTCGACTTGAAGAACTAGAGAGACAGGAAGAATTGCTGGGTGAACTTGATAGTAAGCCTGATACTG
  TGATTGCAAATGGAGAAGATACGACATCTTCTGAAGAGGAAAAGGAAGATCGTAACACAAATGTGAATGC
  GATGCATCAAGTAACAGACTCTCATACTCCTTGTCATAAGGATGTTGCAAGTTCAGAACCATTCAGTGGT
  CAAGTGAATAGTCAGTTGAACTGTTCAGTGAATGGTTCCAGTTCTTACCACAGTGATGATGATGATGATG
  ATGATGATGACGACGACGACGACAACATTGACGACGATGATGGTGATAACGACCATGAGGCTTTAGGGGT
  TGGAGATAATTCTATACCAACAATATATTTTTCACATACTGTTGAGCCTAAGAGGGTCCGAATAAATACT
  GGAAAGAATACCACTTTAAAATTCAGTGAAAAGAAAGAAGAAGCCAAACGTAAACGAAAGAACAGCACTG
  GCAGTGGCCACTCTGCCCAGGAGCTGCCGACCATCAGGACGCCTGCAGACATTTACAGAGCCTTTGTTGA
  TGTTGTGAATGGAGAATATGTCCCTCGCAAATCCATCCTGAAGTCTCGAAGTAGAGAGAATAGTGTGTGT
  AGCGACACTAGTGAAAGCAGTGCTGCTGAATTTGATGATAGGCGGGGAGTTTTGAGGAGTATCAGCTGCG
  AAGAAGCCACTTGCAGTGACACCAGTGAGAGCATTTTGGAAGAGGAACCACAAGAAAATCAAAAGAAACT
  TTTGCCCTTATCAGTAACACCTGAGGCTTTTTCTGGAACTGTTATAGAAAAAGAATTTGTATCACCTTCC
  TTAACACCACCCCCAGCCATTGCTCATCCCGCACTACCCACTATTCCAGAACGAAAGGAAGTTCTGTTGG
  AAGCATCTGAAGAAACTGGAAAGAGGGTTTCAAAGTTTAAAGCTGCCAGATTGCAACAGAAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217842 representing NM_003796
  Red=Cloning site Green=Tags(s)
  
  MEAPTVETPPDPSPPSAPAPALVPLRAPDVARLREEQEKVVTNCQERIQHWKKVDNDYNALRERLSTLPD
  KLSYNIMVPFGPFAFMPGKLVHTNEVTVLLGDNWFAKCSAKQAVGLVEHRKEHVRKTIDDLKKVMKNFES
  RVEFTEDLQKMSDAAGDIVDIREEIKCDFEFKAKHRIAHKPHSKPKTSDIFEADIANDVKSKDLLADKEL
  WARLEELERQEELLGELDSKPDTVIANGEDTTSSEEEKEDRNTNVNAMHQVTDSHTPCHKDVASSEPFSG
  QVNSQLNCSVNGSSSYHSDDDDDDDDDDDDDNIDDDDGDNDHEALGVGDNSIPTIYFSHTVEPKRVRINT
  GKNTTLKFSEKKEEAKRKRKNSTGSGHSAQELPTIRTPADIYRAFVDVVNGEYVPRKSILKSRSRENSVC
  SDTSESSAAEFDDRRGVLRSISCEEATCSDTSESILEEEPQENQKKLLPLSVTPEAFSGTVIEKEFVSPS
  LTPPPAIAHPALPTIPERKEVLLEASEETGKRVSKFKAARLQQKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003796
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_003796.3
• RefSeq Size: 2295 bp
• RefSeq ORF: 1608 bp
• Locus ID: 8725
• UniProt ID: O94763
• Cytogenetics: 19q12
• Domains: DUF232
• Protein Families: Transcription Factors
• MW: 59.7 kDa
• Gene Summary: This gene encodes member of the prefoldin family of molecular chaperones. The encoded protein functions as a scaffolding protein and plays roles in ubiquitination and transcription, in part though interactions with the RNA polymerase II subunit RPB5. This gene may play a role in multiple malignancies including ovarian cancer and hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 22. [provided by RefSeq, Nov 2011]