Melatonin Related Receptor (GPR50) (NM_004224) Human Tagged Lenti ORF Clone

SKU
RC217839L3
Lenti ORF clone of Human G protein-coupled receptor 50 (GPR50), Myc-DDK-tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$1,161.00
3 Weeks*
Specifications
Product Data
Target Symbol Melatonin Related Receptor
Synonyms H9; Mel1c
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC217839).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_004224
ORF Size 1851 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004224.2
RefSeq Size 2020 bp
RefSeq ORF 1854 bp
Locus ID 9248
UniProt ID Q13585
Cytogenetics Xq28
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 67.4 kDa
Summary This gene product belongs to the G-protein coupled receptor 1 family. Even though this protein shares similarity with the melatonin receptors, it does not bind melatonin, however, it inhibits melatonin receptor 1A function through heterodimerization. Polymorphic variants of this gene have been associated with bipolar affective disorder in women. [provided by RefSeq, Jan 2010]
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SKU Description Size Price
RC217839 GPR50 (Myc-DDK-tagged)-Human G protein-coupled receptor 50 (GPR50) 10 ug
$861.00
RC217839L1 Lenti ORF clone of Human G protein-coupled receptor 50 (GPR50), Myc-DDK-tagged 10 ug
$1,161.00
RC217839L2 Lenti ORF clone of Human G protein-coupled receptor 50 (GPR50), mGFP tagged 10 ug
$1,161.00
RC217839L4 Lenti ORF clone of Human G protein-coupled receptor 50 (GPR50), mGFP tagged 10 ug
$1,161.00
RG217839 GPR50 (tGFP-tagged) - Human G protein-coupled receptor 50 (GPR50) 10 ug
$1,061.00
SC318061 GPR50 (untagged)-Human G protein-coupled receptor 50 (GPR50) 10 ug
$863.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.