RALYL (NM_001100392) Human Tagged ORF Clone

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SKU
RC217681
RALYL (Myc-DDK-tagged)-Human RALY RNA binding protein-like (RALYL), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RALYL
Synonyms HNRPCL3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217681 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGGCAAAACACAGACCAGCAACGTCACCAATAAGAATGACCCCAAGTCCATCAACTCCCGTGTTT
TCATCGGCAATCTAAATACGGCAATTGTCAAGAAAGTTGACATTGAAGCCATTTTTTCAAAGTATGGAAA
AATAGTTGGATGTTCCGTTCACAAAGGTTATGCATTTGTACAGTACATGAGTGAGCGACATGCAAGAGCT
GCAGTGGCTGGAGAAAATGCCAGAGTCATCGCCGGCCAACCACTTGATATCAACATGGCAGGAGAGCCCA
AACCATACAGACCAAAACCTGGAAACAAGAGGCCCCTTTCTGCACTTTACAGACTTGAATCAAAGGAACC
TTTCCTGTCTGTTGGCGGTTATGTCTTTGACTATGATTACTACAGAGATGATTTCTACAATCGGTTATTT
GATTACCACGGGCGTGTGCCTCCACCTCCCCGTGCAGTAATTCCGCTGAAGCGTCCCAGAGTGGCAGTCA
CAACGACTCGCAGGGGGAAAGGAGTCTTTTCCATGAAAGGTGGATCGAGATCTACTGCCAGTGGGTCAAC
AGGTTCTAAATTGAAATCAGATGAGTTACAGACCATCAAGAAAGAATTAACCCAGATCAAAACTAAAATT
GACTCCTTGCTAGGGCGCCTGGAGAAGATTGAGAAACAGCAGAAGGCGGAGGCAGAAGCTCAGAAGAAGC
AATTGGAAGAGAGTCTAGTGCTGATCCAAGAGGAATGTGTGTCAGAGATTGCAGATCACTCTACAGAGGA
GCCTGCTGAAGGAGGGCCAGATGCCGATGGAGAAGAGATGACAGATGGGATAGAGGAGGACTTCGATGAA
GATGGGGGTCATGAGCTGTTTCTACAGATAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC217681 protein sequence
Red=Cloning site Green=Tags(s)

MTGKTQTSNVTNKNDPKSINSRVFIGNLNTAIVKKVDIEAIFSKYGKIVGCSVHKGYAFVQYMSERHARA
AVAGENARVIAGQPLDINMAGEPKPYRPKPGNKRPLSALYRLESKEPFLSVGGYVFDYDYYRDDFYNRLF
DYHGRVPPPPRAVIPLKRPRVAVTTTRRGKGVFSMKGGSRSTASGSTGSKLKSDELQTIKKELTQIKTKI
DSLLGRLEKIEKQQKAEAEAQKKQLEESLVLIQEECVSEIADHSTEEPAEGGPDADGEEMTDGIEEDFDE
DGGHELFLQIK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001100392
ORF Size 873 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001100392.2
RefSeq Size 2238 bp
RefSeq ORF 876 bp
Locus ID 138046
UniProt ID Q86SE5
Cytogenetics 8q21.2
MW 32.3 kDa
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Documents
Resources
"NM_001100392" in other vectors (4)
SKU Description Size Price
RC217681L3 Lenti ORF clone of Human RALY RNA binding protein-like (RALYL), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC217681L4 Lenti ORF clone of Human RALY RNA binding protein-like (RALYL), transcript variant 2, mGFP tagged 10 ug
$600.00
RG217681 RALYL (tGFP-tagged) - Human RALY RNA binding protein-like (RALYL), transcript variant 2 10 ug
$500.00
SC316708 RALYL (untagged)-Human RALY RNA binding protein-like (RALYL), transcript variant 2 10 ug
$330.00

Plasmid Map for RC217681

Download sequence for

Cloning scheme for RC217681

Circular map for RC217681

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