FISH (SH3PXD2A) (NM_014631) Human Tagged ORF Clone

CAT#: RC217487

SH3PXD2A (Myc-DDK-tagged)-Human SH3 and PX domains 2A (SH3PXD2A)

Product Images

Circular map for RC217487

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SH3PXD2A (Cat# RC217487, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH3PXD2A (1:2000) (Cat# TA811759). Positive lysates LY415151 (100ug) and LC415151 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY415151) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217487 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FISH
• Synonyms: FISH; SH3MD1; TKS5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217487 representing NM_014631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGCCTACTGCGTGCAGGATGCCACCGTGGTGGACGTGGAGAAGCGGAGGAACCCCTCCAAGCACT
  ACGTATACATAATCAATGTGACCTGGTCTGACTCCACCTCCCAGACTATCTACCGGAGGTACAGCAAGTT
  CTTTGACCTGCAGATGCAGCTTTTGGATAAGTTTCCCATTGAAGGTGGCCAGAAGGACCCCAAGCAAAGG
  ATCATCCCCTTCCTCCCAGGCAAGATCCTCTTCCGCAGAAGCCACATCCGGGACGTAGCTGTGAAGAGAC
  TGAAGCCCATCGATGAATACTGCCGGGCACTTGTCCGGCTGCCCCCCCACATCTCACAGTGTGACGAAGT
  CTTCCGGTTCTTCGAGGCTCGACCCGAGGATGTCAACCCTCCAAAAGAGGACTATGGCAGTTCCAAGAGG
  AAATCAGTGTGGCTGTCCAGCTGGGCTGAGTCGCCCAAGAAGGACGTGACAGGTGCCGACGCCACCGCCG
  AGCCCATGATCCTGGAACAGTACGTGGTGGTGTCCAACTATAAGAAGCAGGAGAACTCGGAGCTGAGCCT
  CCAGGCCGGGGAGGTGGTGGATGTCATCGAGAAGAACGAGAGCGGCTGGTGGTTCGTGAGCACTTCTGAG
  GAGCAGGGCTGGGTCCCTGCCACCTACCTGGAGGCCCAGAATGGTACTCGGGATGACTCCGACATCAACA
  CCTCTAAGACTGGAGAAGAGGAGAAGTATGTCACCGTGCAGCCTTACACCAGCCAAAGCAAGGACGAGAT
  TGGCTTTGAGAAGGGCGTCACAGTGGAGGTGATCCGGAAGAATCTGGAAGGCTGGTGGTATATCAGATAC
  CTGGGCAAAGAGGGCTGGGCGCCAGCATCCTACCTGAAGAAGGCCAAGGATGACCTGCCAACCCGGAAGA
  AGAACCTGGCCGGCCCAGTGGAGATCATTGGGAACATCATGGAGATCAGCAACCTGCTGAACAAGAAGGC
  GTCTGGGGACAAGGAAACTCCACCAGCCGAAGGCGAGGGCCATGAGGCCCCCATTGCCAAGAAGGAGATC
  AGCCTGCCCATCCTCTGCAATGCCTCCAATGGCAGTGCCGTGGGCGTTCCTGACAGGACTGTCTCCAGGC
  TGGCCCAGGGCTCTCCAGCTGTGGCCAGGATTGCCCCTCAGCGGGCCCAGATCAGCTCCCCGAACCTACG
  GACAAGACCTCCACCACGCAGAGAATCCAGCCTGGGGTTCCAACTGCCAAAGCCACCAGAGCCCCCTTCT
  GTTGAGGTGGAGTACTACACCATTGCCGAATTCCAGTCGTGCATTTCCGATGGCATCAGCTTTCGGGGTG
  GACAGAAGGCAGAGGTCATTGATAAGAACTCAGGTGGCTGGTGGTACGTGCAGATCGGTGAGAAGGAGGG
  CTGGGCCCCCGCATCATACATCGATAAGCGCAAGAAGCCCAACCTGAGCCGCCGCACAAGCACGCTGACC
  CGGCCCAAGGTGCCCCCGCCAGCACCCCCCAGCAAGCCCAAGGAGGCCGAGGAGGGCCCTACGGGGGCCA
  GTGAGAGCCAGGACTCCCCGCGGAAGCTCAAGTATGAGGAGCCTGAGTATGACATCCCTGCATTCGGCTT
  TGACTCAGAGCCTGAGCTGAGCGAGGAGCCCGTGGAGGACAGAGCCTCAGGGGAGAGGCGGCCTGCCCAG
  CCCCACCGGCCCTCGCCGGCCTCTTCTCTGCAGCGGGCCCGCTTCAAGGTGGGTGAGTCTTCAGAGGATG
  TGGCCCTGGAAGAGGAGACCATCTATGAGAATGAGGGCTTCCGGCCATATGCAGAGGACACCCTGTCAGC
  CAGAGGCTCCTCCGGGGACAGCGACTCCCCAGGCAGCTCCTCGCTGTCCCTGACCAGGAAAAACTCCCCC
  AAATCAGGCTCCCCCAAGTCATCATCACTCCTAAAGCTCAAGGCAGAGAAGAATGCCCAGGCAGAAATGG
  GGAAGAACCACTCCTCAGCCTCCTTTTCCTCATCCATCACCATCAACACCACTTGCTGCTCCTCCTCTTC
  CTCCTCCTCCTCTTCCTTGTCCAAAACCAGTGGCGACCTGAAGCCCCGCTCTGCTTCGGACGCAGGCATC
  CGCGGCACTCCCAAGGTCAGGGCAAAGAAGGATGCTGATGCGAACGCTGGGCTGACCTCCTGTCCCCGGG
  CCAAGCCATCGGTCCGGCCCAAGCCATTCCTAAACCGAGCAGAGTCGCAGAGCCAAGAGAAGATGGACAT
  CAGCACTTTACGGCGCCAGCTGAGACCCACAGGCCAGCTCCGTGGAGGGCTCAAGGGCTCCAAGAGTGAG
  GATTCGGAGCTGCCCCCGCAGACGGCCTCCGAGGCTCCCAGTGAGGGGTCTAGGAGAAGCTCATCCGACC
  TCATCACCCTCCCAGCCACCACTCCCCCATGTCCCACCAAGAAGGAATGGGAAGGGCCAGCCACCTCGTA
  CATGACATGCAGCGCCTACCAGAAGGTCCAGGACTCGGAGATCAGCTTCCCCGCGGGCGTGGAGGTGCAG
  GTGCTGGAGAAGCAGGAGAGCGGGTGGTGGTATGTGAGGTTTGGGGAGCTGGAGGGCTGGGCCCCTTCCC
  ACTATTTGGTGCTGGATGAGAACGAGCAACCTGACCCCTCTGGCAAAGAGCTGGACACAGTGCCCGCCAA
  GGGCAGGCAGAACGAAGGCAAATCAGACAGCCTGGAGAAGATCGAGAGGCGCGTCCAAGCACTGAACACC
  GTCAACCAGAGCAAGAAGGCCACGCCCCCCATCCCCTCCAAACCTCCCGGGGGCTTCGGCAAGACCTCAG
  GCACTCCAGCGGTGAAGATGAGGAACGGAGTGCGGCAGGTGGCGGTCAGGCCCCAGTCGGTGTTTGTGTC
  CCCGCCACCCAAGGACAACAACCTGTCCTGCGCCCTGCGGAGGAATGAGTCACTCACGGCCACTGATGGC
  CTCCGAGGCGTCCGACGGAACTCCTCCTTTAGCACTGCTCGCTCCGCTGCCGCCGAGGCCAAGGGCCGCC
  TGGCCGAACGGGCTGCCAGCCAGGGTTCAGACTCACCCCTACTGCCCGCCCAGCGCAACAGCATACCCGT
  GTCCCCTGTGCGCCCCAAGCCCATCGAGAAGTCTCAGTTCATCCACAATAACCTCAAAGATGTGTACGTC
  TCTATCGCAGACTACGAGGGGGATGAGGAGACAGCAGGCTTCCAGGAGGGGGTGTCCATGGAGGTTCTGG
  AGAGGAACCCTAATGGCTGGTGGTACTGCCAGATCCTGGATGGTGTGAAGCCCTTCAAAGGCTGGGTGCC
  TTCCAACTACCTTGAGAAAAAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217487 representing NM_014631
  Red=Cloning site Green=Tags(s)
  
  MLAYCVQDATVVDVEKRRNPSKHYVYIINVTWSDSTSQTIYRRYSKFFDLQMQLLDKFPIEGGQKDPKQR
  IIPFLPGKILFRRSHIRDVAVKRLKPIDEYCRALVRLPPHISQCDEVFRFFEARPEDVNPPKEDYGSSKR
  KSVWLSSWAESPKKDVTGADATAEPMILEQYVVVSNYKKQENSELSLQAGEVVDVIEKNESGWWFVSTSE
  EQGWVPATYLEAQNGTRDDSDINTSKTGEEEKYVTVQPYTSQSKDEIGFEKGVTVEVIRKNLEGWWYIRY
  LGKEGWAPASYLKKAKDDLPTRKKNLAGPVEIIGNIMEISNLLNKKASGDKETPPAEGEGHEAPIAKKEI
  SLPILCNASNGSAVGVPDRTVSRLAQGSPAVARIAPQRAQISSPNLRTRPPPRRESSLGFQLPKPPEPPS
  VEVEYYTIAEFQSCISDGISFRGGQKAEVIDKNSGGWWYVQIGEKEGWAPASYIDKRKKPNLSRRTSTLT
  RPKVPPPAPPSKPKEAEEGPTGASESQDSPRKLKYEEPEYDIPAFGFDSEPELSEEPVEDRASGERRPAQ
  PHRPSPASSLQRARFKVGESSEDVALEEETIYENEGFRPYAEDTLSARGSSGDSDSPGSSSLSLTRKNSP
  KSGSPKSSSLLKLKAEKNAQAEMGKNHSSASFSSSITINTTCCSSSSSSSSSLSKTSGDLKPRSASDAGI
  RGTPKVRAKKDADANAGLTSCPRAKPSVRPKPFLNRAESQSQEKMDISTLRRQLRPTGQLRGGLKGSKSE
  DSELPPQTASEAPSEGSRRSSSDLITLPATTPPCPTKKEWEGPATSYMTCSAYQKVQDSEISFPAGVEVQ
  VLEKQESGWWYVRFGELEGWAPSHYLVLDENEQPDPSGKELDTVPAKGRQNEGKSDSLEKIERRVQALNT
  VNQSKKATPPIPSKPPGGFGKTSGTPAVKMRNGVRQVAVRPQSVFVSPPPKDNNLSCALRRNESLTATDG
  LRGVRRNSSFSTARSAAAEAKGRLAERAASQGSDSPLLPAQRNSIPVSPVRPKPIEKSQFIHNNLKDVYV
  SIADYEGDEETAGFQEGVSMEVLERNPNGWWYCQILDGVKPFKGWVPSNYLEKKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014631
• ORF Size: 3315 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014631.2, NP_055446.2
• RefSeq Size: 11264 bp
• RefSeq ORF: 3318 bp
• Locus ID: 9644
• UniProt ID: Q5TCZ1
• Cytogenetics: 10q24.33
• MW: 121.7 kDa
• Gene Summary: Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.[UniProtKB/Swiss-Prot Function]