MAP4K3 (NM_003618) Human Tagged ORF Clone

CAT#: RC217448

MAP4K3 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3)

Product Images

Circular map for RC217448

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  Western blot validation of overexpression lysate (Cat# LY401198) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217448 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MAP4K3 protein (Cat# TP317448). The protein was produced from HEK293T cells transfected with MAP4K3 cDNA clone (Cat# RC217448) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAP4K3
• Synonyms: GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217448 representing NM_003618
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGACTTCGAGCTGATTCAGCGCATCGGCA
  GCGGCACCTACGGCGACGTCTACAAGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAAT
  AAAATTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATCATGATGAAAGACTGTAAACAC
  CCAAATATTGTTGCTTATTTTGGAAGCTATCTCAGGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTG
  GAGGTGGTTCTTTACAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATATGTTAG
  CAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAAAAATGCACAGAGATATAAAGGGAGCT
  AACATTCTATTAACGGATAATGGTCATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTA
  CAATTGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAGTTGCAGCTGTTGAGAG
  GAAGGGGGGTTACAATCAACTCTGTGATCTCTGGGCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTT
  CAGCCTCCTATGTTTGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTCAGCCTC
  CTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCACTTTGTGAAAATGGCACTTACCAAAAA
  TCCGAAAAAAAGACCTACTGCTGAAAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCT
  TTGGCAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCATGATTTCGATGATGATG
  ATCCTGAGCCTCTTGTTGCTGTACCACATAGAATTCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAAC
  ACGCTCAGAGATAACCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCACATCAT
  GAACTTCCCGACAGTGATGGTTTTTTGGACAGTTCAGAAGAAATATACTACACTGCAAGATCTAATCTGG
  ATCTGCAACTGGAATATGGACAAGGACACCAAGGTGGTTACTTTTTAGGTGCAAACAAGAGTCTTCTCAA
  GTCTGTTGAAGAAGAATTGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGATGATGAT
  GAATCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCCACCAAAGCCTAAGTCTATCTTCA
  TACCACAGGAAATGCATTCTACTGAGGATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTCAGGGAG
  CCCAGCAAAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCAGATTACCCCCACACAAACCTGTTGCC
  TTAGGAAATGGAATGAGCTCCTTCCAGTTAAATGGTGAACGAGATGGCTCATTATGTCAACAACAGAATG
  AACATAGAGGCACAAACCTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATGGTCTTCC
  TCCAACACCTAAAGTGCATATGGGTGCATGTTTTTCAAAAGTTTTTAATGGGTGTCCCTTGAAAATTCAC
  TGTGCATCATCATGGATAAACCCAGATACAAGAGATCAGTACTTGATATTTGGTGCCGAAGAAGGGATTT
  ATACCCTCAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTCCCTCGAAGGTGTACATGGTT
  GTATGTAATGAACAATTGCTTGCTATCAATATCTGGTAAAGCTTCTCAGCTTTATTCCCATAATTTACCA
  GGGCTTTTTGATTATGCAAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCACACAAACTCCCTGACA
  GAATACTGCCAAGGAAATTTTCTGTATCAGCAAAAATCCCTGAAACCAAATGGTGCCAGAAGTGTTGTGT
  TGTAAGAAATCCTTACACGGGCCATAAATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTATTAGAA
  TGGGTTGAACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCATGTCCACTTAGAA
  TGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCCTTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGA
  CTTCAACCAAGTGGTTCGATTTGAGACGGTCAATCCAAATTCTACCTCTTCATGGTTTACAGAATCAGAT
  ACCCCACAGACAAATGTTACTCATGTAACCCAACTGGAGAGAGATACCATCCTTGTATGCTTGGACTGTT
  GTATAAAAATAGTAAATCTCCAAGGAAGATTAAAATCTAGCAGGAAATTGTCATCAGAACTCACCTTTGA
  TTTCCAGATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACATGGAATGCAAGGT
  AGAAGTTTTAGATCTAATGAGGTAACACAAGAAATTTCAGATAGCACAAGAATTTTCAGGCTGCTTGGAT
  CTGACAGGGTCGTGGTTTTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATAGCAATTTGTACATCCT
  GGCGGGTCATGAAAACAGTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217448 representing NM_003618
  Red=Cloning site Green=Tags(s)
  
  MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKH
  PNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGA
  NILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAEL
  QPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRS
  LAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHH
  ELPDSDGFLDSSEEIYYTARSNLDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDD
  ESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVA
  LGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIH
  CASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLP
  GLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLE
  WVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESD
  TPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQG
  RSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003618
• ORF Size: 2682 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003618.4
• RefSeq Size: 4141 bp
• RefSeq ORF: 2685 bp
• Locus ID: 8491
• UniProt ID: Q8IVH8
• Cytogenetics: 2p22.1
• Domains: pkinase, CNH, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• MW: 101.1 kDa
• Gene Summary: This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]