MAP4K3 (NM_003618) Human Tagged ORF Clone

CAT#: RC217448

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MAP4K3 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3)



  "NM_003618" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 851.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MAP4K3
Synonyms GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217448 representing NM_003618
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGACTTCGAGCTGATTCAGCGCATCGGCA
GCGGCACCTACGGCGACGTCTACAAGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAAT
AAAATTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATCATGATGAAAGACTGTAAACAC
CCAAATATTGTTGCTTATTTTGGAAGCTATCTCAGGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTG
GAGGTGGTTCTTTACAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATATGTTAG
CAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAAAAATGCACAGAGATATAAAGGGAGCT
AACATTCTATTAACGGATAATGGTCATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTA
CAATTGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAGTTGCAGCTGTTGAGAG
GAAGGGGGGTTACAATCAACTCTGTGATCTCTGGGCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTT
CAGCCTCCTATGTTTGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTCAGCCTC
CTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCACTTTGTGAAAATGGCACTTACCAAAAA
TCCGAAAAAAAGACCTACTGCTGAAAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCT
TTGGCAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCATGATTTCGATGATGATG
ATCCTGAGCCTCTTGTTGCTGTACCACATAGAATTCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAAC
ACGCTCAGAGATAACCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCACATCAT
GAACTTCCCGACAGTGATGGTTTTTTGGACAGTTCAGAAGAAATATACTACACTGCAAGATCTAATCTGG
ATCTGCAACTGGAATATGGACAAGGACACCAAGGTGGTTACTTTTTAGGTGCAAACAAGAGTCTTCTCAA
GTCTGTTGAAGAAGAATTGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGATGATGAT
GAATCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCCACCAAAGCCTAAGTCTATCTTCA
TACCACAGGAAATGCATTCTACTGAGGATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTCAGGGAG
CCCAGCAAAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCAGATTACCCCCACACAAACCTGTTGCC
TTAGGAAATGGAATGAGCTCCTTCCAGTTAAATGGTGAACGAGATGGCTCATTATGTCAACAACAGAATG
AACATAGAGGCACAAACCTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATGGTCTTCC
TCCAACACCTAAAGTGCATATGGGTGCATGTTTTTCAAAAGTTTTTAATGGGTGTCCCTTGAAAATTCAC
TGTGCATCATCATGGATAAACCCAGATACAAGAGATCAGTACTTGATATTTGGTGCCGAAGAAGGGATTT
ATACCCTCAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTCCCTCGAAGGTGTACATGGTT
GTATGTAATGAACAATTGCTTGCTATCAATATCTGGTAAAGCTTCTCAGCTTTATTCCCATAATTTACCA
GGGCTTTTTGATTATGCAAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCACACAAACTCCCTGACA
GAATACTGCCAAGGAAATTTTCTGTATCAGCAAAAATCCCTGAAACCAAATGGTGCCAGAAGTGTTGTGT
TGTAAGAAATCCTTACACGGGCCATAAATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTATTAGAA
TGGGTTGAACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCATGTCCACTTAGAA
TGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCCTTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGA
CTTCAACCAAGTGGTTCGATTTGAGACGGTCAATCCAAATTCTACCTCTTCATGGTTTACAGAATCAGAT
ACCCCACAGACAAATGTTACTCATGTAACCCAACTGGAGAGAGATACCATCCTTGTATGCTTGGACTGTT
GTATAAAAATAGTAAATCTCCAAGGAAGATTAAAATCTAGCAGGAAATTGTCATCAGAACTCACCTTTGA
TTTCCAGATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACATGGAATGCAAGGT
AGAAGTTTTAGATCTAATGAGGTAACACAAGAAATTTCAGATAGCACAAGAATTTTCAGGCTGCTTGGAT
CTGACAGGGTCGTGGTTTTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATAGCAATTTGTACATCCT
GGCGGGTCATGAAAACAGTTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217448 representing NM_003618
Red=Cloning site Green=Tags(s)

MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKH
PNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGA
NILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAEL
QPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRS
LAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHH
ELPDSDGFLDSSEEIYYTARSNLDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDD
ESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVA
LGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIH
CASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLP
GLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLE
WVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESD
TPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQG
RSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003618
ORF Size 2682 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003618.4
RefSeq Size 4141 bp
RefSeq ORF 2685 bp
Locus ID 8491
UniProt ID Q8IVH8
Cytogenetics 2p22.1
Domains pkinase, CNH, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways MAPK signaling pathway
MW 101.1 kDa
Gene Summary This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

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