PTPN5 (NM_032781) Human Tagged ORF Clone

CAT#: RC217371

PTPN5 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 2

Product Images

Circular map for RC217371

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  Western blot validation of overexpression lysate (Cat# LY403199) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217371 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PTPN5 protein (Cat# TP317371). The protein was produced from HEK293T cells transfected with PTPN5 cDNA clone (Cat# RC217371) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTPN5
• Synonyms: PTPSTEP; STEP; STEP61
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217371 representing NM_032781
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTATGAGGGAGCCAGGAGTGAGAGAGAGAACCACGCTGCTGATGACTCCGAGGGAGGGGCCCTGG
  ACATGTGCTGCAGTGAGAGGCTACCGGGTCTCCCCCAGCCGATAGTGATGGAGGCACTGGACGAGGCTGA
  AGGGCTCCAGGACTCACAGAGAGAGATGCCGCCACCCCCTCCTCCCTCGCCGCCCTCAGATCCAGCTCAG
  AAGCCACCACCTCGAGGCGCTGGGAGCCACTCCCTCACTGTCAGGAGCAGCCTGTGCCTGTTCGCTGCCT
  CACAGTTCCTGCTTGCCTGTGGGGTGCTCTGGTTCAGCGGTTATGGCCACATCTGGTCACAGAACGCCAC
  AAACCTCGTCTCCTCTTTGCTGACGCTCCTGAAACAGCTGGAACCCACGGCCTGGCTTGACTCTGGGACG
  TGGGGAGTCCCCAGTCTGCTGCTGGTCTTTCTGTCCGTGGGCCTGGTCCTCGTTACCACCCTGGTGTGGC
  ACCTCCTGAGGACACCCCCAGAGCCACCCACCCCACTGCCCCCTGAGGACAGGCGCCAGTCAGTGAGCCG
  CCAGCCCTCCTTCACCTACTCAGAGTGGATGGAGGAGAAGATCGAGGATGACTTCCTGGACCTCGACCCG
  GTGCCCGAGACTCCTGTGTTTGATTGTGTGATGGACATCAAGCCTGAGGCTGACCCCACCTCACTCACCG
  TCAAGTCCATGGGTCTGCAGGAGAGGAGGGGTTCCAATGTCTCCCTGACCCTGGACATGTGCACTCCGGG
  CTGCAACGAGGAGGGCTTTGGCTATCTCATGTCCCCACGTGAGGAGTCCGCCCGCGAGTACCTGCTCAGC
  GCCTCCCGTGTCCTCCAAGCAGAAGAGCTTCATGAAAAGGCCCTGGACCCTTTCCTGCTGCAGGCGGAAT
  TCTTTGAAATCCCCATGAACTTTGTGGATCCGAAAGAGTACGACATCCCTGGGCTGGTGCGGAAGAACCG
  GTACAAAACCATACTTCCCAACCCTCACAGCAGAGTGTGTCTGACCTCACCAGACCCTGACGACCCTCTG
  AGTTCCTACATCAATGCCAACTACATCCGGGGCTATGGTGGGGAGGAGAAGGTGTACATCGCCACTCAGG
  GACCCATCGTCAGCACGGTCGCCGACTTCTGGCGCATGGTGTGGCAGGAGCACACGCCCATCATTGTCAT
  GATCACCAACATCGAGGAGATGAACGAGAAATGCACCGAGTATTGGCCGGAGGAGCAGGTGGCGTACGAC
  GGTGTTGAGATCACTGTGCAGAAAGTCATTCACACGGAGGATTACCGGCTGCGACTCATCTCCCTCAAGA
  GTGGGACTGAGGAGCGAGGCCTGAAGCATTACTGGTTCACATCCTGGCCCGACCAGAAGACCCCAGACCG
  GGCCCCCCCACTCCTGCACCTGGTGCGGGAGGTGGAGGAGGCAGCCCAGCAGGAGGGGCCCCACTGTGCC
  CCCATCATCGTCCACTGCAGTGCAGGGATTGGGAGGACCGGCTGCTTCATTGCCACCAGCATCTGCTGCC
  AGCAGCTGCGGCAGGAGGGTGTAGTGGACATCCTGAAGACCACGTGCCAGCTCCGTCAGGACAGGGGCGG
  CATGATCCAGACATGCGAGCAGTACCAGTTTGTGCACCACGTCATGAGCCTCTACGAAAAGCAGCTGTCC
  CACCAGTCCCCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217371 representing NM_032781
  Red=Cloning site Green=Tags(s)
  
  MNYEGARSERENHAADDSEGGALDMCCSERLPGLPQPIVMEALDEAEGLQDSQREMPPPPPPSPPSDPAQ
  KPPPRGAGSHSLTVRSSLCLFAASQFLLACGVLWFSGYGHIWSQNATNLVSSLLTLLKQLEPTAWLDSGT
  WGVPSLLLVFLSVGLVLVTTLVWHLLRTPPEPPTPLPPEDRRQSVSRQPSFTYSEWMEEKIEDDFLDLDP
  VPETPVFDCVMDIKPEADPTSLTVKSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLMSPREESAREYLLS
  ASRVLQAEELHEKALDPFLLQAEFFEIPMNFVDPKEYDIPGLVRKNRYKTILPNPHSRVCLTSPDPDDPL
  SSYINANYIRGYGGEEKVYIATQGPIVSTVADFWRMVWQEHTPIIVMITNIEEMNEKCTEYWPEEQVAYD
  GVEITVQKVIHTEDYRLRLISLKSGTEERGLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCA
  PIIVHCSAGIGRTGCFIATSICCQQLRQEGVVDILKTTCQLRQDRGGMIQTCEQYQFVHHVMSLYEKQLS
  HQSPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032781
• ORF Size: 1695 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032781.4
• RefSeq Size: 2965 bp
• RefSeq ORF: 1698 bp
• Locus ID: 84867
• UniProt ID: P54829
• Cytogenetics: 11p15.1
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: MAPK signaling pathway
• MW: 63.4 kDa
• Gene Summary: May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.[UniProtKB/Swiss-Prot Function]