BARHL2 (NM_020063) Human Tagged ORF Clone

CAT#: RC217326

BARHL2 (Myc-DDK-tagged)-Human BarH-like homeobox 2 (BARHL2)

Product images

Western blot validation of overexpression lysate (Cat# LY412652) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217326 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BARHL2 protein (Cat# TP317326). The protein was produced from HEK293T cells transfected with BARHL2 cDNA clone (Cat# RC217326) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Product Name: BARHL2 (NM_020063) Human Tagged ORF Clone
• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BARHL2
• Synonyms: BarH-like 2; BarH-like homeobox 2; OTTHUMP00000011704
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217326 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATGGAAGGGGCCAGCGGGTCGAGTTTTGGAATAGACACGATTTTGTCCAGTGCCAGTTCAGGCA
  GCCCAGGCATGATGAATGGAGATTTCCGCCCGCTCGGTGAGGCCAGGACCGCGGATTTTAGGAGTCAGGC
  CACCCCATCTCCCTGTTCGGAGATTGATACCGTAGGGACGGCGCCTTCTTCTCCTATCTCAGTCACCATG
  GAGCCCCCGGAGCCGCATCTGGTAGCAGACGCGACCCAGCATCATCACCACCTCCACCACAGCCAGCAGC
  CGCCGCCGCCGGCCGCGGCCCCGACGCAAAGTTTGCAGCCTTTGCCCCAACAGCAGCAGCCGCTGCCGCC
  ACAGCAGCCGCCGCCGCCGCCCCCCCAGCAGCTGGGCTCGGCCGCCTCGGCCCCCAGGACTTCCACGTCT
  TCTTTTTTAATTAAGGACATCTTGGGCGACAGCAAACCTCTGGCGGCATGTGCACCCTACAGCACCAGCG
  TATCCTCTCCCCACCACACCCCGAAGCAGGAGAGCAACGCAGTGCACGAGAGCTTCAGGCCAAAGCTCGA
  GCAGGAGGACAGCAAGACCAAACTCGACAAGCGGGAGGATTCCCAGAGCGACATCAAATGCCACGGGACA
  AAGGAGGAAGGAGACCGGGAGATTACGAGTAGCCGTGAGAGTCCCCCTGTGAGAGCCAAGAAGCCTCGAA
  AAGCAAGGACAGCTTTTTCCGACCACCAGCTCAATCAACTGGAGCGTAGCTTTGAGCGGCAGAAGTACCT
  GAGCGTGCAGGATCGCATGGACCTGGCTGCAGCGCTCAACCTCACTGACACCCAAGTCAAGACCTGGTAC
  CAGAACCGCAGGACCAAGTGGAAGCGGCAGACAGCGGTGGGCCTGGAGTTGCTGGCCGAGGCAGGGAACT
  ACTCGGCGCTGCAGAGGATGTTTCCATCGCCTTATTTCTATCACCCAAGCCTGCTGGGCAGCATGGACAG
  CACTACGGCGGCGGCGGCTGCCGCTGCCATGTACAGCAGCATGTACCGGACTCCTCCAGCACCCCATCCC
  CAGCTGCAGCGGCCCCTGGTGCCCCGTGTGCTCATCCACGGCCTAGGGCCTGGGGGACAGCCAGCCCTTA
  ATCCATTGTCCAGCCCCATCCCAGGCACCCCACACCCCCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217326 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTMEGASGSSFGIDTILSSASSGSPGMMNGDFRPLGEARTADFRSQATPSPCSEIDTVGTAPSSPISVTM
  EPPEPHLVADATQHHHHLHHSQQPPPPAAAPTQSLQPLPQQQQPLPPQQPPPPPPQQLGSAASAPRTSTS
  SFLIKDILGDSKPLAACAPYSTSVSSPHHTPKQESNAVHESFRPKLEQEDSKTKLDKREDSQSDIKCHGT
  KEEGDREITSSRESPPVRAKKPRKARTAFSDHQLNQLERSFERQKYLSVQDRMDLAAALNLTDTQVKTWY
  QNRRTKWKRQTAVGLELLAEAGNYSALQRMFPSPYFYHPSLLGSMDSTTAAAAAAAMYSSMYRTPPAPHP
  QLQRPLVPRVLIHGLGPGGQPALNPLSSPIPGTPHPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• Tag: Myc-DDK
• ACCN: NM_020063
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020063.1, NP_064447.1
• RefSeq Size: 1496 bp
• RefSeq ORF: 1164 bp
• Locus ID: 343472
• Cytogenetics: 1p22.2
• MW: 42 kDa