FGFR2 (NM_022970) Human Tagged ORF Clone
Lenti ORF clone of Human fibroblast growth factor receptor 2 (FGFR2), transcript variant 2, mGFP tagged
|Product Name||FGFR2 (NM_022970) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||BBDS; BEK; BFR-1; CD332; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||AscI-MluI Cloning Scheme for this gene|
|ORF Size||2466 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_022970.2, NP_075259.2, NP_075259.4|
|RefSeq Size||4647 bp|
|RefSeq ORF||2469 bp|
|Domains||pkinase, TyrKc, S_TKc, ig, IGc2, IG|
|Protein Families||Druggable Genome, Protein Kinase, Secreted Protein, Transmembrane|
|Protein Pathways||Endocytosis, MAPK signaling pathway, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton|
|Gene Summary||The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]|
|cDNA Clone Resources|
|SC316314||FGFR2 (untagged)-Human fibroblast growth factor receptor 2 (FGFR2), transcript variant 2||
|SC318304||FGFR2 (untagged)-Human fibroblast growth factor receptor 2 (FGFR2), transcript variant 2||
|RC217280||FGFR2 (Myc-DDK-tagged)-Human fibroblast growth factor receptor 2 (FGFR2), transcript variant 2||
|RG217280||FGFR2 (GFP-tagged) - Human fibroblast growth factor receptor 2 (FGFR2), transcript variant 2||
|RC217280L1||Lenti ORF clone of Human fibroblast growth factor receptor 2 (FGFR2), transcript variant 2, Myc-DDK-tagged||
|RC217280L2||Lenti ORF clone of Human fibroblast growth factor receptor 2 (FGFR2), transcript variant 2, mGFP tagged||
|RC217280L3||Lenti ORF clone of Human fibroblast growth factor receptor 2 (FGFR2), transcript variant 2, Myc-DDK-tagged||