PTCH1 (NM_001083605) Human Tagged Lenti ORF Clone

CAT#: RC217224L3

  • LentiORF®

Lenti-ORF clone of PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1c'


  "NM_001083605" in other vectors (4)

Reconstitution Protocol

USD 1,541.00

6 Weeks*

Size
    • 10 ug

Product Images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PTCH1
Synonyms BCNS; NBCCS; PTC; PTC1; PTCH
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RC217224).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NM_001083605
ORF Size 3888 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001083605.1
RefSeq Size 7658 bp
RefSeq ORF 3891 bp
Locus ID 5727
UniProt ID Q13635
Cytogenetics 9q22.32
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
MW 144.2 kDa
Gene Summary This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.