KHSRP (NM_003685) Human Tagged ORF Clone

CAT#: RC217220

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KHSRP (Myc-DDK-tagged)-Human KH-type splicing regulatory protein (KHSRP)



  "NM_003685" in other vectors (6)

Special Offer: 20% off on control vector. Use code "Shuttle20".

USD 884.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KHSRP
Synonyms FBP2; FUBP2; KSRP; p75
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217220 representing NM_003685
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGACTACAGCACGGGAGGACCCCCGCCCGGGCCGCCGCCGCCCGCCGGCGGGGGCGGGGGAGCCG
GAGGCGCCGGGGGAGGCCCTCCGCCGGGCCCGCCAGGCGCGGGGGACCGGGGCGGCGGCGGTCCCGGCGG
CGGCGGCCCGGGCGGGGGGTCGGCCGGGGGCCCCTCTCAGCCACCCGGCGGAGGCGGCCCGGGAATCCGC
AAGGACGCTTTCGCCGACGCCGTGCAGCGGGCCCGCCAGATTGCAGCCAAAATTGGAGGCGATGCTGCCA
CGACAGTGAATAACAGCACTCCTGATTTTGGTTTTGGGGGCCAAAAGAGACAGTTGGAAGATGGAGATCA
ACCGGAGAGCAAGAAGCTGGCTTCCCAGGGAGACTCAATCAGTTCTCAACTTGGACCCATCCATCCTCCC
CCAAGGACTTCAATGACAGAAGAGTACAGGGTCCCAGACGGCATGGTGGGCCTGATCATTGGCAGAGGAG
GTGAACAAATTAACAAAATCCAACAGGATTCAGGCTGCAAAGTACAGATTTCTCCAGACAGCGGTGGCCT
ACCCGAGCGCAGTGTGTCCTTGACAGGAGCCCCAGAATCTGTCCAGAAAGCCAAGATGATGCTGGATGAC
ATTGTGTCTCGGGGTCGTGGGGGCCCCCCAGGACAGTTCCACGACAACGCCAACGGGGGCCAGAACGGCA
CCGTGCAGGAGATCATGATCCCCGCGGGCAAGGCCGGCCTGGTCATTGGCAAGGGCGGGGAGACCATTAA
GCAGCTGCAGGAACGCGCTGGAGTGAAGATGATCTTAATTCAGGACGGATCTCAGAATACGAATGTGGAC
AAACCTCTCCGCATCATTGGGGATCCTTACAAAGTGCAGCAAGCCTGTGAGATGGTGATGGACATCCTCC
GGGAACGTGACCAAGGCGGCTTTGGGGACCGGAATGAGTACGGATCTCGGATTGGCGGAGGCATCGATGT
GCCAGTGCCCAGGCATTCTGTTGGCGTGGTCATTGGCCGGAGTGGAGAGATGATCAAGAAGATCCAGAAT
GATGCTGGCGTGCGGATACAGTTCAAGCAAGATGACGGGACAGGGCCCGAGAAGATTGCTCATATAATGG
GGCCCCCAGACAGGTGCGAGCACGCAGCCCGGATCATCAACGACCTCCTCCAGAGCCTCAGGAGTGGTCC
CCCAGGTCCTCCAGGGGGTCCAGGCATGCCCCCGGGGGGCCGAGGCCGAGGAAGAGGCCAAGGCAATTGG
GGTCCCCCTGGCGGGGAGATGACCTTCTCCATCCCCACTCACAAGTGTGGGCTGGTCATCGGCCGAGGTG
GCGAGAATGTGAAAGCCATAAACCAGCAGACGGGAGCCTTCGTAGAGATCTCCCGGCAGCTGCCACCCAA
CGGGGACCCCAACTTCAAGTTGTTCATCATCCGGGGTTCACCCCAGCAGATTGACCACGCCAAGCAGCTT
ATCGAGGAAAAGATCGAGGGTCCTCTCTGCCCAGTTGGACCAGGCCCAGGTGGCCCAGGCCCTGCTGGCC
CAATGGGGCCCTTCAATCCTGGGCCCTTCAACCAGGGGCCACCCGGGGCTCCCCCACATGCCGGGGGGCC
CCCTCCTCACCAGTACCCACCCCAGGGCTGGGGCAATACCTACCCCCAGTGGCAGCCGCCTGCTCCTCAT
GACCCAAGCAAAGCAGCTGCAGCGGCCGCGGACCCCAACGCCGCGTGGGCCGCCTACTACTCACACTACT
ACCAGCAGCCCCCGGGCCCCGTCCCCGGCCCCGCACCGGCCCCTGCGGCCCCACCGGCTCAGGGTGAGCC
CCCTCAGCCCCCACCCACCGGCCAGTCGGACTACACTAAGGCCTGGGAAGAGTATTACAAAAAGATCGGC
CAGCAGCCCCAGCAGCCCGGAGCGCCCCCACAGCAGGACTACACGAAGGCTTGGGAGGAGTACTACAAGA
AGCAAGCGCAAGTGGCCACCGGAGGGGGTCCAGGAGCTCCCCCAGGCTCCCAGCCAGACTACAGTGCCGC
CTGGGCGGAATATTACAGACAGCAGGCCGCTTACTACGGACAGACCCCAGGTCCTGGCGGCCCCCAGCCG
CCGCCCACGCAGCAGGGACAGCAGCAGGCTCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217220 representing NM_003685
Red=Cloning site Green=Tags(s)

MSDYSTGGPPPGPPPPAGGGGGAGGAGGGPPPGPPGAGDRGGGGPGGGGPGGGSAGGPSQPPGGGGPGIR
KDAFADAVQRARQIAAKIGGDAATTVNNSTPDFGFGGQKRQLEDGDQPESKKLASQGDSISSQLGPIHPP
PRTSMTEEYRVPDGMVGLIIGRGGEQINKIQQDSGCKVQISPDSGGLPERSVSLTGAPESVQKAKMMLDD
IVSRGRGGPPGQFHDNANGGQNGTVQEIMIPAGKAGLVIGKGGETIKQLQERAGVKMILIQDGSQNTNVD
KPLRIIGDPYKVQQACEMVMDILRERDQGGFGDRNEYGSRIGGGIDVPVPRHSVGVVIGRSGEMIKKIQN
DAGVRIQFKQDDGTGPEKIAHIMGPPDRCEHAARIINDLLQSLRSGPPGPPGGPGMPPGGRGRGRGQGNW
GPPGGEMTFSIPTHKCGLVIGRGGENVKAINQQTGAFVEISRQLPPNGDPNFKLFIIRGSPQQIDHAKQL
IEEKIEGPLCPVGPGPGGPGPAGPMGPFNPGPFNQGPPGAPPHAGGPPPHQYPPQGWGNTYPQWQPPAPH
DPSKAAAAAADPNAAWAAYYSHYYQQPPGPVPGPAPAPAAPPAQGEPPQPPPTGQSDYTKAWEEYYKKIG
QQPQQPGAPPQQDYTKAWEEYYKKQAQVATGGGPGAPPGSQPDYSAAWAEYYRQQAAYYGQTPGPGGPQP
PPTQQGQQQAQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003685
ORF Size 2133 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003685.3
RefSeq Size 3009 bp
RefSeq ORF 2136 bp
Locus ID 8570
UniProt ID Q92945
Cytogenetics 19p13.3
Protein Families Druggable Genome, Transcription Factors
MW 72.9 kDa
Gene Summary The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization (Min et al., 1997 [PubMed 9136930]; Gherzi et al., 2004 [PubMed 15175153]).[supplied by OMIM, Apr 2010]

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