KHSRP (NM_003685) Human Tagged ORF Clone

CAT#: RC217220

KHSRP (Myc-DDK-tagged)-Human KH-type splicing regulatory protein (KHSRP)

Product Images

Circular map for RC217220

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  Western blot validation of overexpression lysate (Cat# LY418503) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217220 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KHSRP protein (Cat# TP317220). The protein was produced from HEK293T cells transfected with KHSRP cDNA clone (Cat# RC217220) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KHSRP
• Synonyms: FBP2; FUBP2; KSRP; p75
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217220 representing NM_003685
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGACTACAGCACGGGAGGACCCCCGCCCGGGCCGCCGCCGCCCGCCGGCGGGGGCGGGGGAGCCG
  GAGGCGCCGGGGGAGGCCCTCCGCCGGGCCCGCCAGGCGCGGGGGACCGGGGCGGCGGCGGTCCCGGCGG
  CGGCGGCCCGGGCGGGGGGTCGGCCGGGGGCCCCTCTCAGCCACCCGGCGGAGGCGGCCCGGGAATCCGC
  AAGGACGCTTTCGCCGACGCCGTGCAGCGGGCCCGCCAGATTGCAGCCAAAATTGGAGGCGATGCTGCCA
  CGACAGTGAATAACAGCACTCCTGATTTTGGTTTTGGGGGCCAAAAGAGACAGTTGGAAGATGGAGATCA
  ACCGGAGAGCAAGAAGCTGGCTTCCCAGGGAGACTCAATCAGTTCTCAACTTGGACCCATCCATCCTCCC
  CCAAGGACTTCAATGACAGAAGAGTACAGGGTCCCAGACGGCATGGTGGGCCTGATCATTGGCAGAGGAG
  GTGAACAAATTAACAAAATCCAACAGGATTCAGGCTGCAAAGTACAGATTTCTCCAGACAGCGGTGGCCT
  ACCCGAGCGCAGTGTGTCCTTGACAGGAGCCCCAGAATCTGTCCAGAAAGCCAAGATGATGCTGGATGAC
  ATTGTGTCTCGGGGTCGTGGGGGCCCCCCAGGACAGTTCCACGACAACGCCAACGGGGGCCAGAACGGCA
  CCGTGCAGGAGATCATGATCCCCGCGGGCAAGGCCGGCCTGGTCATTGGCAAGGGCGGGGAGACCATTAA
  GCAGCTGCAGGAACGCGCTGGAGTGAAGATGATCTTAATTCAGGACGGATCTCAGAATACGAATGTGGAC
  AAACCTCTCCGCATCATTGGGGATCCTTACAAAGTGCAGCAAGCCTGTGAGATGGTGATGGACATCCTCC
  GGGAACGTGACCAAGGCGGCTTTGGGGACCGGAATGAGTACGGATCTCGGATTGGCGGAGGCATCGATGT
  GCCAGTGCCCAGGCATTCTGTTGGCGTGGTCATTGGCCGGAGTGGAGAGATGATCAAGAAGATCCAGAAT
  GATGCTGGCGTGCGGATACAGTTCAAGCAAGATGACGGGACAGGGCCCGAGAAGATTGCTCATATAATGG
  GGCCCCCAGACAGGTGCGAGCACGCAGCCCGGATCATCAACGACCTCCTCCAGAGCCTCAGGAGTGGTCC
  CCCAGGTCCTCCAGGGGGTCCAGGCATGCCCCCGGGGGGCCGAGGCCGAGGAAGAGGCCAAGGCAATTGG
  GGTCCCCCTGGCGGGGAGATGACCTTCTCCATCCCCACTCACAAGTGTGGGCTGGTCATCGGCCGAGGTG
  GCGAGAATGTGAAAGCCATAAACCAGCAGACGGGAGCCTTCGTAGAGATCTCCCGGCAGCTGCCACCCAA
  CGGGGACCCCAACTTCAAGTTGTTCATCATCCGGGGTTCACCCCAGCAGATTGACCACGCCAAGCAGCTT
  ATCGAGGAAAAGATCGAGGGTCCTCTCTGCCCAGTTGGACCAGGCCCAGGTGGCCCAGGCCCTGCTGGCC
  CAATGGGGCCCTTCAATCCTGGGCCCTTCAACCAGGGGCCACCCGGGGCTCCCCCACATGCCGGGGGGCC
  CCCTCCTCACCAGTACCCACCCCAGGGCTGGGGCAATACCTACCCCCAGTGGCAGCCGCCTGCTCCTCAT
  GACCCAAGCAAAGCAGCTGCAGCGGCCGCGGACCCCAACGCCGCGTGGGCCGCCTACTACTCACACTACT
  ACCAGCAGCCCCCGGGCCCCGTCCCCGGCCCCGCACCGGCCCCTGCGGCCCCACCGGCTCAGGGTGAGCC
  CCCTCAGCCCCCACCCACCGGCCAGTCGGACTACACTAAGGCCTGGGAAGAGTATTACAAAAAGATCGGC
  CAGCAGCCCCAGCAGCCCGGAGCGCCCCCACAGCAGGACTACACGAAGGCTTGGGAGGAGTACTACAAGA
  AGCAAGCGCAAGTGGCCACCGGAGGGGGTCCAGGAGCTCCCCCAGGCTCCCAGCCAGACTACAGTGCCGC
  CTGGGCGGAATATTACAGACAGCAGGCCGCTTACTACGGACAGACCCCAGGTCCTGGCGGCCCCCAGCCG
  CCGCCCACGCAGCAGGGACAGCAGCAGGCTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217220 representing NM_003685
  Red=Cloning site Green=Tags(s)
  
  MSDYSTGGPPPGPPPPAGGGGGAGGAGGGPPPGPPGAGDRGGGGPGGGGPGGGSAGGPSQPPGGGGPGIR
  KDAFADAVQRARQIAAKIGGDAATTVNNSTPDFGFGGQKRQLEDGDQPESKKLASQGDSISSQLGPIHPP
  PRTSMTEEYRVPDGMVGLIIGRGGEQINKIQQDSGCKVQISPDSGGLPERSVSLTGAPESVQKAKMMLDD
  IVSRGRGGPPGQFHDNANGGQNGTVQEIMIPAGKAGLVIGKGGETIKQLQERAGVKMILIQDGSQNTNVD
  KPLRIIGDPYKVQQACEMVMDILRERDQGGFGDRNEYGSRIGGGIDVPVPRHSVGVVIGRSGEMIKKIQN
  DAGVRIQFKQDDGTGPEKIAHIMGPPDRCEHAARIINDLLQSLRSGPPGPPGGPGMPPGGRGRGRGQGNW
  GPPGGEMTFSIPTHKCGLVIGRGGENVKAINQQTGAFVEISRQLPPNGDPNFKLFIIRGSPQQIDHAKQL
  IEEKIEGPLCPVGPGPGGPGPAGPMGPFNPGPFNQGPPGAPPHAGGPPPHQYPPQGWGNTYPQWQPPAPH
  DPSKAAAAAADPNAAWAAYYSHYYQQPPGPVPGPAPAPAAPPAQGEPPQPPPTGQSDYTKAWEEYYKKIG
  QQPQQPGAPPQQDYTKAWEEYYKKQAQVATGGGPGAPPGSQPDYSAAWAEYYRQQAAYYGQTPGPGGPQP
  PPTQQGQQQAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003685
• ORF Size: 2133 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003685.3
• RefSeq Size: 3009 bp
• RefSeq ORF: 2136 bp
• Locus ID: 8570
• UniProt ID: Q92945
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 72.9 kDa
• Gene Summary: The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization (Min et al., 1997 [PubMed 9136930]; Gherzi et al., 2004 [PubMed 15175153]).[supplied by OMIM, Apr 2010]