PAPSS1 (NM_005443) Human Tagged ORF Clone

CAT#: RC217023

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PAPSS1 (Myc-DDK-tagged)-Human 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1)



  "NM_005443" in other vectors (4)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 847.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PAPSS1
Synonyms ATPSK1; PAPSS; SK1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217023 representing NM_005443
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATCCCCGGGAGCCTGTGCAAGAAAGTCAAACTGAGCAATAACGCGCAGAACTGGGGAATGCAGA
GAGCAACCAATGTCACCTACCAAGCCCATCATGTCAGCAGGAACAAGAGAGGTCAGGTGGTGGGGACCAG
AGGTGGCTTTCGTGGTTGCACAGTTTGGCTAACAGGCTTGTCTGGAGCGGGAAAGACTACTGTGAGCATG
GCCTTGGAGGAGTACCTGGTTTGTCATGGTATTCCATGCTACACTCTGGATGGTGACAATATTCGTCAAG
GTCTCAATAAAAATCTTGGCTTTAGTCCTGAAGACAGAGAAGAGAATGTTCGACGCATCGCAGAAGTTGC
TAAACTGTTTGCAGATGCTGGCTTAGTGTGCATCACAAGTTTCATATCACCTTACACTCAGGATCGCAAC
AATGCAAGGCAAATTCATGAAGGTGCAAGTTTACCGTTTTTTGAAGTATTTGTTGATGCTCCTCTGCATG
TTTGTGAACAGAGGGATGTCAAAGGACTCTACAAAAAAGCCCGGGCAGGAGAAATTAAAGGTTTCACTGG
GATCGATTCTGAATATGAAAAGCCAGAGGCCCCTGAGTTGGTGCTGAAAACAGACTCCTGTGATGTAAAT
GACTGTGTCCAGCAAGTTGTGGAACTTCTACAGGAACGGGATATTGTACCTGTGGATGCATCTTATGAAG
TAAAAGAACTATATGTGCCAGAAAATAAACTTCATTTGGCAAAAACAGATGCGGAAACATTACCAGCACT
GAAAATTAATAAAGTGGATATGCAGTGGGTGCAGGTTTTGGCAGAAGGTTGGGCAACCCCATTGAATGGC
TTTATGAGAGAGAGGGAGTACTTGCAGTGCCTTCATTTTGATTGTCTTCTGGATGGAGGTGTCATTAACT
TGTCAGTACCTATAGTTCTGACTGCGACTCATGAAGATAAAGAGAGGCTGGACGGCTGTACAGCATTTGC
TCTGATGTATGAGGGCCGCCGTGTGGCCATTCTTCGCAATCCAGAGTTTTTTGAGCACAGGAAAGAGGAG
CGCTGTGCCAGACAGTGGGGAACGACATGCAAGAACCACCCCTATATTAAGATGGTGATGGAACAAGGAG
ATTGGCTGATTGGAGGAGATCTTCAAGTCTTGGATCGAGTTTATTGGAATGATGGTCTTGATCAGTATCG
TCTTACTCCTACTGAGCTAAAGCAGAAATTTAAAGATATGAATGCTGATGCTGTCTTTGCATTTCAACTA
CGCAACCCAGTGCACAATGGACATGCCCTGTTAATGCAGGATACCCATAAGCAACTTCTAGAGAGGGGCT
ACCGGCGCCCTGTCCTCCTCCTCCACCCTCTGGGTGGCTGGACAAAGGATGACGATGTTCCTTTGATGTG
GCGTATGAAGCAGCATGCTGCAGTGTTGGAGGAAGGAGTTCTGAATCCTGAGACGACAGTGGTGGCCATC
TTCCCATCTCCCATGATGTATGCTGGACCAACTGAGGTCCAGTGGCATTGCAGAGCACGGATGGTTGCAG
GAGCCAACTTTTACATTGTTGGACGAGACCCTGCTGGCATGCCTCATCCAGAAACAGGGAAGGATCTTTA
TGAGCCAAGTCATGGTGCCAAAGTGCTGACGATGGCCCCTGGTTTAATCACTTTGGAAATAGTTCCCTTT
CGAGTTGCAGCTTACAACAAGAAAAAGAAGCGTATGGACTACTATGACTCTGAACACCATGAAGACTTTG
AATTTATTTCAGGAACACGAATGCGCAAACTTGCTCGAGAAGGCCAGAAACCACCTGAAGGTTTCATGGC
TCCCAAGGCTTGGACCGTGCTGACAGAATACTACAAATCCTTGGAGAAAGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217023 representing NM_005443
Red=Cloning site Green=Tags(s)

MEIPGSLCKKVKLSNNAQNWGMQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSM
ALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYTQDRN
NARQIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVN
DCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAEGWATPLNG
FMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALMYEGRRVAILRNPEFFEHRKEE
RCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQL
RNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAI
FPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPF
RVAAYNKKKKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSLEKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005443
ORF Size 1872 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005443.5
RefSeq Size 2558 bp
RefSeq ORF 1875 bp
Locus ID 9061
UniProt ID O43252
Cytogenetics 4q25
Domains ATP-sulfurylase, APS_kinase
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Purine metabolism, Selenoamino acid metabolism, Sulfur metabolism
MW 70.7 kDa
Gene Summary Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008]

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