Plakophilin 1 (PKP1) (NM_001005337) Human Tagged ORF Clone

CAT#: RC216972

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PKP1 (Myc-DDK-tagged)-Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1a



  "NM_001005337" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PKP1
Synonyms B6P; EDSFS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC216972 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCACTCGCCGCTCAAGACCGCCTTGGCGTACGAATGCTTCCAGGACCAGGACAACTCCACGTTGG
CTTTGCCGTCGGACCAAAAGATGAAAACAGGCACGTCTGGCAGGCAGCGCGTGCAGGAGCAGGTGATGAT
GACCGTCAAGCGGCAGAAGTCCAAGTCTTCCCAGTCGTCCACCCTGAGCCACTCCAATCGAGGTTCCATG
TATGATGGCTTGGCTGACAATTACAACTATGGGACCACCAGCAGGAGCAGCTACTACTCCAAGTTCCAGG
CAGGGAATGGCTCATGGGGATATCCGATCTACAATGGAACCCTCAAGCGGGAGCCTGACAACAGGCGCTT
CAGCTCCTACAGCCAGATGGAGAACTGGAGCCGGCACTACCCCCGGGGCAGCTGTAACACCACCGGCGCA
GGCAGCGACATCTGCTTCATGCAGAAAATCAAGGCGAGCCGCAGTGAGCCCGACCTCTACTGTGACCCAC
GGGGCACCCTGCGCAAGGGCACGCTGGGCAGCAAGGGCCAGAAGACCACCCAGAACCGCTACAGCTTTTA
CAGCACCTGCAGTGGTCAGAAGGCCATAAAGAAGTGCCCTGTGCGCCCGCCCTCTTGTGCCTCCAAGCAG
GACCCTGTGTATATCCCGCCCATCTCCTGCAACAAGGACCTGTCCTTTGGCCACTCTAGGGCCAGCTCCA
AGATCTGCAGTGAGGACATCGAGTGCAGTGGGCTGACCATCCCCAAGGCTGTGCAGTACCTGAGCTCCCA
GGATGAGAAGTACCAGGCCATTGGGGCCTATTACATCCAGCATACCTGCTTCCAGGATGAATCTGCCAAG
CAACAGGTCTATCAGCTGGGAGGCATCTGCAAGCTGGTGGACCTCCTCCGCAGCCCCAACCAGAACGTCC
AGCAGGCTGCGGCAGGGGCCCTGCGCAACCTGGTGTTCAGGAGCACCACCAACAAGCTGGAGACCCGGAG
GCAGAATGGGATCCGCGAGGCAGTCAGCCTCCTGAGGAGAACCGGGAACGCCGAGATCCAGAAGCAGCTG
ACTGGGCTGCTCTGGAACCTGTCTTCCACTGACGAGCTGAAGGAGGAACTCATTGCCGACGCCCTGCCTG
TTCTGGCCGACCGCGTCATCATTCCCTTCTCTGGCTGGTGCGATGGCAATAGCAACATGTCCCGGGAAGT
GGTGGACCCTGAGGTCTTCTTCAATGCCACAGGCTGCTTGAGGAACCTGAGCTCGGCCGATGCAGGCCGC
CAGACCATGCGTAACTACTCAGGGCTCATTGATTCCCTCATGGCCTATGTCCAGAACTGTGTAGCGGCCA
GCCGCTGTGACGACAAGTCTGTGGAAAACTGCATGTGTGTTCTGCACAACCTCTCCTACCGCCTGGACGC
CGAGGTGCCCACCCGCTACCGCCAGCTGGAGTATAACGCCCGCAACGCCTACACCGAGAAGTCCTCCACT
GGCTGCTTCAGCAACAAGAGCGACAAGATGATGAACAACAACTATGACTGCCCCCTGCCTGAGGAAGAGA
CCAACCCCAAGGGCAGCGGCTGGTTGTACCATTCAGATGCCATCCGCACCTACCTGAACCTCATGGGCAA
GAGCAAGAAAGATGCTACCCTGGAGGCCTGTGCTGGTGCCCTGCAGAACCTGACAGCCAGCAAGGGGCTG
ATGTCCAGTGGCATGAGCCAGTTGATTGGGCTGAAGGAAAAGGGCCTGCCACAAATTGCCCGCCTCCTGC
AATCTGGCAACTCTGATGTGGTGCGGTCCGGAGCCTCCCTCCTGAGCAACATGTCCCGCCACCCTCTGCT
GCACAGAGTGATGGGGAACCAGGTGTTCCCGGAGGTGACCAGGCTCCTCACCAGCCACACTGGCAATACC
AGCAACTCCGAAGACATCTTGTCCTCGGCCTGCTACACTGTGAGGAACCTGATGGCCTCGCAGCCACAAC
TGGCCAAGCAGTACTTCTCCAGCAGCATGCTCAACAACATCATCAACCTGTGCCGAAGCAGTGCCTCACC
CAAGGCCGCAGAAGCTGCCCGGCTTCTCCTGTCTGACATGTGGTCCAGCAAGGAACTGCAGGGTGTCCTC
AGACAGCAAGGTTTCGATAGGAACATGCTGGGAACCTTAGCTGGGGCCAACAGCCTCAGGAACTTCACCT
CCCGATTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC216972 protein sequence
Red=Cloning site Green=Tags(s)

MNHSPLKTALAYECFQDQDNSTLALPSDQKMKTGTSGRQRVQEQVMMTVKRQKSKSSQSSTLSHSNRGSM
YDGLADNYNYGTTSRSSYYSKFQAGNGSWGYPIYNGTLKREPDNRRFSSYSQMENWSRHYPRGSCNTTGA
GSDICFMQKIKASRSEPDLYCDPRGTLRKGTLGSKGQKTTQNRYSFYSTCSGQKAIKKCPVRPPSCASKQ
DPVYIPPISCNKDLSFGHSRASSKICSEDIECSGLTIPKAVQYLSSQDEKYQAIGAYYIQHTCFQDESAK
QQVYQLGGICKLVDLLRSPNQNVQQAAAGALRNLVFRSTTNKLETRRQNGIREAVSLLRRTGNAEIQKQL
TGLLWNLSSTDELKEELIADALPVLADRVIIPFSGWCDGNSNMSREVVDPEVFFNATGCLRNLSSADAGR
QTMRNYSGLIDSLMAYVQNCVAASRCDDKSVENCMCVLHNLSYRLDAEVPTRYRQLEYNARNAYTEKSST
GCFSNKSDKMMNNNYDCPLPEEETNPKGSGWLYHSDAIRTYLNLMGKSKKDATLEACAGALQNLTASKGL
MSSGMSQLIGLKEKGLPQIARLLQSGNSDVVRSGASLLSNMSRHPLLHRVMGNQVFPEVTRLLTSHTGNT
SNSEDILSSACYTVRNLMASQPQLAKQYFSSSMLNNIINLCRSSASPKAAEAARLLLSDMWSSKELQGVL
RQQGFDRNMLGTLAGANSLRNFTSRF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001005337
ORF Size 2178 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001005337.3
RefSeq Size 5384 bp
RefSeq ORF 2181 bp
Locus ID 5317
UniProt ID Q13835
Cytogenetics 1q32.1
Protein Families Druggable Genome
MW 80.5 kDa
Gene Summary This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

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