Plakophilin 1 (PKP1) (NM_001005337) Human Tagged ORF Clone

CAT#: RC216972

PKP1 (Myc-DDK-tagged)-Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1a

Product Images

Circular map for RC216972

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  Western blot validation of overexpression lysate (Cat# LY423863) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216972 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PKP1 protein (Cat# TP316972). The protein was produced from HEK293T cells transfected with PKP1 cDNA clone (Cat# RC216972) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plakophilin 1
• Synonyms: B6P; EDSFS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216972 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCACTCGCCGCTCAAGACCGCCTTGGCGTACGAATGCTTCCAGGACCAGGACAACTCCACGTTGG
  CTTTGCCGTCGGACCAAAAGATGAAAACAGGCACGTCTGGCAGGCAGCGCGTGCAGGAGCAGGTGATGAT
  GACCGTCAAGCGGCAGAAGTCCAAGTCTTCCCAGTCGTCCACCCTGAGCCACTCCAATCGAGGTTCCATG
  TATGATGGCTTGGCTGACAATTACAACTATGGGACCACCAGCAGGAGCAGCTACTACTCCAAGTTCCAGG
  CAGGGAATGGCTCATGGGGATATCCGATCTACAATGGAACCCTCAAGCGGGAGCCTGACAACAGGCGCTT
  CAGCTCCTACAGCCAGATGGAGAACTGGAGCCGGCACTACCCCCGGGGCAGCTGTAACACCACCGGCGCA
  GGCAGCGACATCTGCTTCATGCAGAAAATCAAGGCGAGCCGCAGTGAGCCCGACCTCTACTGTGACCCAC
  GGGGCACCCTGCGCAAGGGCACGCTGGGCAGCAAGGGCCAGAAGACCACCCAGAACCGCTACAGCTTTTA
  CAGCACCTGCAGTGGTCAGAAGGCCATAAAGAAGTGCCCTGTGCGCCCGCCCTCTTGTGCCTCCAAGCAG
  GACCCTGTGTATATCCCGCCCATCTCCTGCAACAAGGACCTGTCCTTTGGCCACTCTAGGGCCAGCTCCA
  AGATCTGCAGTGAGGACATCGAGTGCAGTGGGCTGACCATCCCCAAGGCTGTGCAGTACCTGAGCTCCCA
  GGATGAGAAGTACCAGGCCATTGGGGCCTATTACATCCAGCATACCTGCTTCCAGGATGAATCTGCCAAG
  CAACAGGTCTATCAGCTGGGAGGCATCTGCAAGCTGGTGGACCTCCTCCGCAGCCCCAACCAGAACGTCC
  AGCAGGCTGCGGCAGGGGCCCTGCGCAACCTGGTGTTCAGGAGCACCACCAACAAGCTGGAGACCCGGAG
  GCAGAATGGGATCCGCGAGGCAGTCAGCCTCCTGAGGAGAACCGGGAACGCCGAGATCCAGAAGCAGCTG
  ACTGGGCTGCTCTGGAACCTGTCTTCCACTGACGAGCTGAAGGAGGAACTCATTGCCGACGCCCTGCCTG
  TTCTGGCCGACCGCGTCATCATTCCCTTCTCTGGCTGGTGCGATGGCAATAGCAACATGTCCCGGGAAGT
  GGTGGACCCTGAGGTCTTCTTCAATGCCACAGGCTGCTTGAGGAACCTGAGCTCGGCCGATGCAGGCCGC
  CAGACCATGCGTAACTACTCAGGGCTCATTGATTCCCTCATGGCCTATGTCCAGAACTGTGTAGCGGCCA
  GCCGCTGTGACGACAAGTCTGTGGAAAACTGCATGTGTGTTCTGCACAACCTCTCCTACCGCCTGGACGC
  CGAGGTGCCCACCCGCTACCGCCAGCTGGAGTATAACGCCCGCAACGCCTACACCGAGAAGTCCTCCACT
  GGCTGCTTCAGCAACAAGAGCGACAAGATGATGAACAACAACTATGACTGCCCCCTGCCTGAGGAAGAGA
  CCAACCCCAAGGGCAGCGGCTGGTTGTACCATTCAGATGCCATCCGCACCTACCTGAACCTCATGGGCAA
  GAGCAAGAAAGATGCTACCCTGGAGGCCTGTGCTGGTGCCCTGCAGAACCTGACAGCCAGCAAGGGGCTG
  ATGTCCAGTGGCATGAGCCAGTTGATTGGGCTGAAGGAAAAGGGCCTGCCACAAATTGCCCGCCTCCTGC
  AATCTGGCAACTCTGATGTGGTGCGGTCCGGAGCCTCCCTCCTGAGCAACATGTCCCGCCACCCTCTGCT
  GCACAGAGTGATGGGGAACCAGGTGTTCCCGGAGGTGACCAGGCTCCTCACCAGCCACACTGGCAATACC
  AGCAACTCCGAAGACATCTTGTCCTCGGCCTGCTACACTGTGAGGAACCTGATGGCCTCGCAGCCACAAC
  TGGCCAAGCAGTACTTCTCCAGCAGCATGCTCAACAACATCATCAACCTGTGCCGAAGCAGTGCCTCACC
  CAAGGCCGCAGAAGCTGCCCGGCTTCTCCTGTCTGACATGTGGTCCAGCAAGGAACTGCAGGGTGTCCTC
  AGACAGCAAGGTTTCGATAGGAACATGCTGGGAACCTTAGCTGGGGCCAACAGCCTCAGGAACTTCACCT
  CCCGATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216972 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNHSPLKTALAYECFQDQDNSTLALPSDQKMKTGTSGRQRVQEQVMMTVKRQKSKSSQSSTLSHSNRGSM
  YDGLADNYNYGTTSRSSYYSKFQAGNGSWGYPIYNGTLKREPDNRRFSSYSQMENWSRHYPRGSCNTTGA
  GSDICFMQKIKASRSEPDLYCDPRGTLRKGTLGSKGQKTTQNRYSFYSTCSGQKAIKKCPVRPPSCASKQ
  DPVYIPPISCNKDLSFGHSRASSKICSEDIECSGLTIPKAVQYLSSQDEKYQAIGAYYIQHTCFQDESAK
  QQVYQLGGICKLVDLLRSPNQNVQQAAAGALRNLVFRSTTNKLETRRQNGIREAVSLLRRTGNAEIQKQL
  TGLLWNLSSTDELKEELIADALPVLADRVIIPFSGWCDGNSNMSREVVDPEVFFNATGCLRNLSSADAGR
  QTMRNYSGLIDSLMAYVQNCVAASRCDDKSVENCMCVLHNLSYRLDAEVPTRYRQLEYNARNAYTEKSST
  GCFSNKSDKMMNNNYDCPLPEEETNPKGSGWLYHSDAIRTYLNLMGKSKKDATLEACAGALQNLTASKGL
  MSSGMSQLIGLKEKGLPQIARLLQSGNSDVVRSGASLLSNMSRHPLLHRVMGNQVFPEVTRLLTSHTGNT
  SNSEDILSSACYTVRNLMASQPQLAKQYFSSSMLNNIINLCRSSASPKAAEAARLLLSDMWSSKELQGVL
  RQQGFDRNMLGTLAGANSLRNFTSRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005337
• ORF Size: 2178 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001005337.3
• RefSeq Size: 5384 bp
• RefSeq ORF: 2181 bp
• Locus ID: 5317
• UniProt ID: Q13835
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome
• MW: 80.5 kDa
• Gene Summary: This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]