FAM83D (NM_030919) Human Tagged ORF Clone

CAT#: RC216954

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FAM83D (Myc-DDK-tagged)-Human family with sequence similarity 83, member D (FAM83D)



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 646.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FAM83D
Synonyms C20orf129; CHICA; dJ616B8.3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC216954 representing NM_030919
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGGGCCTGTTTGATTCAAAGGTTGCCTATAAAGCGGGACTGCACGCCGGTTTTTGTCCGAGGGC
TGTCGAGTCCGAGCGCCGCCATGGCTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTGCCTGTC
GCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCACGGCGCCTGGCTCTGGAGGAGCTGGTG
GCGGGCGGCCCCGAAGCCTTCGCGGCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATG
AGGTGCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGGCGGCGGCGGCGGCCGA
GGACTCGTTCGGCTCCTCGCACGACTGCTCTTCGGGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCA
CCGCTGTTGGAGCTTGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCGAGACGC
ACTTCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGCAAGGACGCTCTGCGCCAGCAGCTCCG
CTCGGCGCGAGAGGTGATTGCAGTGGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAA
GAAATATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTCCTCTCTCAATTTCTGG
ATATGTGCATGGATCTGAAAGTTCATCCTGAACAGGAAAAGTTAATGACAGTTCGGACTATCACAGGAAA
TATCTACTATGCAAGGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATTGATGGC
ATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGGCAAATTAAACAGCAGTAACTTGGTAA
TTCTGTCTGGCCAAGTGGTTGAACACTTTGATCTGGAGTTCCGAATCCTGTATGCCCAGTCCAAGCCCAT
CAGCCCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCACCAACCGAAAACCACAG
TCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGATGCGGCTGGCTAGGCTGTCAAGTACTCCCAGGAAGG
CGGACCTGGACCCAGAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTCCTCTAC
TGTTAGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCCAGAGCAGAAAGGCCATTGACGCTGCC
ACTCAAACAGAGCCAGGAGAGGAGATGCCAGGGCTGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCA
CCACAGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTCAAACCACGATTTGGTC
CAGATCGACCACTACTCAGACTGACATGGATGAGAACATTCTCTTTCCTCGAGGAACTCAATCTACAGAA
GGGTCACCAGTCTCAAAAATGTCTGTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGTCTTCCC
AAGGCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGAACCACTGACTTCCACAATCCTGGCTA
TCCCAAGTACCTGGGCACCCCCCACCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAG
CGGCAATTCCACTTCGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATGCTGTCAAGGAGAACAC
TCTTTACTGAAAACCACCTTGGCCTTCATTCTGGCAATTTCAGCAGAGTTAATTTGCTTGCTGTTAGAGA
TGTAGCACTTTATCCTTCCTATCAG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC216954 representing NM_030919
Red=Cloning site Green=Tags(s)

MARACLIQRLPIKRDCTPVFVRGLSSPSAAMALLSEGLDEVPAACLSPCGPPNPTELFSESRRLALEELV
AGGPEAFAAFLRRERLARFLNPDEVHAILRAAERPGEEGAAAAAAAEDSFGSSHDCSSGTYFPEQSDLEP
PLLELGWPAFYQGAYRGATRVETHFQPRGAGEGGPYGCKDALRQQLRSAREVIAVVMDVFTDIDIFRDLQ
EICRKQGVAVYILLDQALLSQFLDMCMDLKVHPEQEKLMTVRTITGNIYYARSGTKIIGKVHEKFTLIDG
IRVATGSYSFTWTDGKLNSSNLVILSGQVVEHFDLEFRILYAQSKPISPKLLSHFQSSNKFDHLTNRKPQ
SKELTLGNLLRMRLARLSSTPRKADLDPEMPAEGKAERKPHDCESSTVSEEDYFSSHRDELQSRKAIDAA
TQTEPGEEMPGLSVSEVGTQTSITTACAGTQTAVITRIASSQTTIWSRSTTTQTDMDENILFPRGTQSTE
GSPVSKMSVSRSSSLKSSSSVSSQGSVASSTGSPASIRTTDFHNPGYPKYLGTPHLELYLSDSLRNLNKE
RQFHFAGIRSRLNHMLAMLSRRTLFTENHLGLHSGNFSRVNLLAVRDVALYPSYQ

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_030919
ORF Size 1845 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size 2445 bp
RefSeq ORF 1758 bp
Locus ID 81610
UniProt ID Q9H4H8
Cytogenetics 20q11.23
Protein Families Druggable Genome
MW 67.5 kDa
Gene Summary Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC (PubMed:24344117). May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade (PubMed:25646692). May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22.[UniProtKB/Swiss-Prot Function]
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