SATB2 (NM_015265) Human Tagged ORF Clone

CAT#: RC216909

SATB2 (Myc-DDK-tagged)-Human SATB homeobox 2 (SATB2), transcript variant 2

Product Images

Circular map for RC216909

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SATB2 (Cat# RC216909, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB2 (Cat# TA800843)(1:2000). Positive lysates LY414656 (100ug) and LC414656 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY414656) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216909 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SATB2
• Synonyms: GLSS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216909 representing NM_015265
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCGGAGCGAGAGCCCGTGTCTGCGGGACAGCCCCGACCGGCGGAGCGGCAGCCCGGACGTCA
  AGGGGCCTCCCCCAGTGAAGGTGGCCCGGCTGGAGCAGAACGGCAGCCCCATGGGAGCCCGCGGGAGGCC
  CAACGGCGCCGTGGCCAAGGCCGTGGGAGGTTTGATGATTCCTGTCTTTTGTGTCGTGGAGCAGTTGGAC
  GGCTCTCTTGAATATGACAACAGAGAAGAACACGCCGAGTTTGTCCTGGTGCGGAAAGATGTGCTTTTTA
  GCCAGCTGGTGGAGACTGCGCTCCTGGCCCTGGGGTATTCTCACAGCTCTGCGGCCCAGGCCCAAGGAAT
  AATCAAGCTGGGAAGGTGGAACCCTCTCCCCCTCAGTTATGTGACAGATGCACCCGACGCGACAGTGGCC
  GACATGCTACAAGATGTCTATCATGTTGTGACGTTGAAAATCCAATTACAAAGTTGTTCAAAGTTGGAAG
  ACTTGCCTGCGGAGCAGTGGAACCATGCCACAGTCCGCAATGCCTTAAAGGAACTGCTCAAAGAGATGAA
  CCAGAGCACATTAGCCAAAGAATGCCCTCTCTCCCAGAGTATGATTTCATCCATTGTAAATAGCACATAT
  TATGCCAATGTGTCAGCAACCAAGTGCCAGGAGTTTGGGAGATGGTATAAAAAGTACAAGAAGATTAAAG
  TGGAAAGAGTGGAACGAGAAAACCTTTCAGACTATTGTGTTCTGGGCCAGCGTCCAATGCATTTACCAAA
  TATGAACCAGCTGGCATCCCTGGGGAAAACCAACGAACAGTCTCCTCACAGCCAAATTCACCACAGTACT
  CCAATCCGAAACCAAGTGCCCGCATTACAGCCCATCATGAGCCCTGGTCTTCTTTCTCCCCAGCTTAGTC
  CACAACTTGTAAGGCAACAAATAGCCATGGCCCATCTGATAAACCAACAGATTGCCGTTAGCCGGCTCCT
  GGCTCACCAGCATCCTCAAGCCATCAACCAGCAGTTCCTGAACCATCCACCCATCCCCAGAGCAGTTAAG
  CCAGAGCCAACCAACTCTTCCGTGGAAGTCTCTCCAGATATCTACCAGCAAGTCAGAGATGAGCTGAAGA
  GGGCCAGTGTGTCCCAAGCTGTCTTTGCAAGAGTGGCATTCAACCGCACACAGGGATTGTTGTCTGAGAT
  TCTGCGTAAGGAAGAAGACCCTCGGACAGCCTCTCAGTCTCTTCTAGTAAACCTGAGGGCCATGCAGAAT
  TTCCTCAATCTGCCAGAAGTGGAGCGAGATCGCATCTACCAGGATGAGAGGGAGCGGAGCATGAATCCCA
  ATGTGAGCATGGTCTCCTCGGCCTCCAGCAGTCCCAGCTCCTCCCGAACCCCTCAGGCCAAAACCTCGAC
  ACCGACAACAGACCTCCCTATTAAGGTGGACGGCGCCAACATCAACATCACAGCTGCCATTTATGACGAG
  ATCCAACAGGAGATGAAAAGGGCCAAGGTGTCTCAAGCCCTGTTTGCCAAAGTGGCTGCAAATAAAAGTC
  AGGGCTGGCTGTGTGAACTGCTCCGCTGGAAGGAGAACCCAAGCCCAGAAAACCGCACCCTCTGGGAAAA
  CCTCTGTACCATCCGTCGCTTCCTGAACCTTCCCCAGCATGAGAGGGATGTCATCTATGAGGAGGAGTCA
  AGGCATCACCACAGCGAACGCATGCAACACGTGGTCCAGCTTCCCCCTGAGCCGGTGCAGGTACTTCATA
  GACAGCAGTCTCAGCCAGCCAAGGAGAGTTCCCCTCCCAGAGAAGAAGCGCCTCCCCCACCTCCTCCGAC
  TGAAGACAGTTGTGCCAAAAAGCCCCGGTCTCGCACAAAGATCTCCTTAGAAGCCCTGGGGATCCTCCAA
  AGCTTTATTCATGATGTAGGCCTGTACCCAGACCAGGAAGCCATCCACACTCTTTCGGCTCAGCTGGATC
  TCCCCAAACACACCATCATCAAGTTCTTCCAGAACCAGCGGTACCACGTGAAGCACCACGGGAAGCTGAA
  AGAGCACCTGGGCTCCGCGGTGGACGTGGCTGAATATAAGGACGAGGAGCTGCTGACCGAGTCAGAGGAG
  AACGACAGCGAGGAAGGCTCCGAGGAGATGTACAAAGTGGAGGCTGAGGAGGAAAATGCTGACAAAAGCA
  AGGCAGCACCTGCCGAAATTGACCAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216909 representing NM_015265
  Red=Cloning site Green=Tags(s)
  
  MERRSESPCLRDSPDRRSGSPDVKGPPPVKVARLEQNGSPMGARGRPNGAVAKAVGGLMIPVFCVVEQLD
  GSLEYDNREEHAEFVLVRKDVLFSQLVETALLALGYSHSSAAQAQGIIKLGRWNPLPLSYVTDAPDATVA
  DMLQDVYHVVTLKIQLQSCSKLEDLPAEQWNHATVRNALKELLKEMNQSTLAKECPLSQSMISSIVNSTY
  YANVSATKCQEFGRWYKKYKKIKVERVERENLSDYCVLGQRPMHLPNMNQLASLGKTNEQSPHSQIHHST
  PIRNQVPALQPIMSPGLLSPQLSPQLVRQQIAMAHLINQQIAVSRLLAHQHPQAINQQFLNHPPIPRAVK
  PEPTNSSVEVSPDIYQQVRDELKRASVSQAVFARVAFNRTQGLLSEILRKEEDPRTASQSLLVNLRAMQN
  FLNLPEVERDRIYQDERERSMNPNVSMVSSASSSPSSSRTPQAKTSTPTTDLPIKVDGANINITAAIYDE
  IQQEMKRAKVSQALFAKVAANKSQGWLCELLRWKENPSPENRTLWENLCTIRRFLNLPQHERDVIYEEES
  RHHHSERMQHVVQLPPEPVQVLHRQQSQPAKESSPPREEAPPPPPPTEDSCAKKPRSRTKISLEALGILQ
  SFIHDVGLYPDQEAIHTLSAQLDLPKHTIIKFFQNQRYHVKHHGKLKEHLGSAVDVAEYKDEELLTESEE
  NDSEEGSEEMYKVEAEEENADKSKAAPAEIDQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015265
• ORF Size: 2199 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015265.4
• RefSeq Size: 4999 bp
• RefSeq ORF: 2202 bp
• Locus ID: 23314
• UniProt ID: Q9UPW6
• Cytogenetics: 2q33.1
• Protein Families: Transcription Factors
• MW: 82.4 kDa
• Gene Summary: This gene encodes a DNA binding protein that specifically binds nuclear matrix attachment regions. The encoded protein is involved in transcription regulation and chromatin remodeling. Defects in this gene are associated with isolated cleft palate and cognitive disability. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Feb 2010]