TRPM4 (NM_017636) Human Tagged ORF Clone

CAT#: RC216888

TRPM4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1

Product Images

Circular map for RC216888

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRPM4 (Cat# RC216888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPM4(Cat# TA500381). Positive lysates LY413636 (100ug) and LC413636 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413636) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216888 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TRPM4 protein (Cat# TP316888). The protein was produced from HEK293T cells transfected with TRPM4 cDNA clone (Cat# RC216888) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPM4
• Synonyms: EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216888 representing NM_017636
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTGCCGGAGAAGGAGCAGAGCTGGATCCCCAAGATCTTCAAGAAGAAGACCTGCACGACGTTCA
  TAGTTGACTCCACAGATCCGGGAGGGACCTTGTGCCAGTGTGGGCGCCCCCGGACCGCCCACCCCGCAGT
  GGCCATGGAGGATGCCTTCGGGGCAGCCGTGGTGACCGTGTGGGACAGCGATGCACACACCACGGAGAAG
  CCCACCGATGCCTACGGAGAGCTGGACTTCACGGGGGCCGGCCGCAAGCACAGCAATTTCCTCCGGCTCT
  CTGACCGAACGGATCCAGCTGCAGTTTATAGTCTGGTCACACGCACATGGGGCTTCCGTGCCCCGAACCT
  GGTGGTGTCAGTGCTGGGGGGATCGGGGGGCCCCGTCCTCCAGACCTGGCTGCAGGACCTGCTGCGTCGT
  GGGCTGGTGCGGGCTGCCCAGAGCACAGGAGCCTGGATTGTCACTGGGGGTCTGCACACGGGCATCGGCC
  GGCATGTTGGTGTGGCTGTACGGGACCATCAGATGGCCAGCACTGGGGGCACCAAGGTGGTGGCCATGGG
  TGTGGCCCCCTGGGGTGTGGTCCGGAATAGAGACACCCTCATCAACCCCAAGGGCTCGTTCCCTGCGAGG
  TACCGGTGGCGCGGTGACCCGGAGGACGGGGTCCAGTTTCCCCTGGACTACAACTACTCGGCCTTCTTCC
  TGGTGGACGACGGCACACACGGCTGCCTGGGGGGCGAGAACCGCTTCCGCTTGCGCCTGGAGTCCTACAT
  CTCACAGCAGAAGACGGGCGTGGGAGGGACTGGAATTGACATCCCTGTCCTGCTCCTCCTGATTGATGGT
  GATGAGAAGATGTTGACGCGAATAGAGAACGCCACCCAGGCTCAGCTCCCATGTCTCCTCGTGGCTGGCT
  CAGGGGGAGCTGCGGACTGCCTGGCGGAGACCCTGGAAGACACTCTGGCCCCAGGGAGTGGGGGAGCCAG
  GCAAGGCGAAGCCCGAGATCGAATCAGGCGTTTCTTTCCCAAAGGGGACCTTGAGGTCCTGCAGGCCCAG
  GTGGAGAGGATTATGACCCGGAAGGAGCTCCTGACAGTCTATTCTTCTGAGGATGGGTCTGAGGAATTCG
  AGACCATAGTTTTGAAGGCCCTTGTGAAGGCCTGTGGGAGCTCGGAGGCCTCAGCCTACCTGGATGAGCT
  GCGTTTGGCTGTGGCTTGGAACCGCGTGGACATTGCCCAGAGTGAACTCTTTCGGGGGGACATCCAATGG
  CGGTCCTTCCATCTCGAAGCTTCCCTCATGGACGCCCTGCTGAATGACCGGCCTGAGTTCGTGCGCTTGC
  TCATTTCCCACGGCCTCAGCCTGGGCCACTTCCTGACCCCGATGCGCCTGGCCCAACTCTACAGCGCGGC
  GCCCTCCAACTCGCTCATCCGCAACCTTTTGGACCAGGCGTCCCACAGCGCAGGCACCAAAGCCCCAGCC
  CTAAAAGGGGGAGCTGCGGAGCTCCGGCCCCCTGACGTGGGGCATGTGCTGAGGATGCTGCTGGGGAAGA
  TGTGCGCGCCGAGGTACCCCTCCGGGGGCGCCTGGGACCCTCACCCAGGCCAGGGCTTCGGGGAGAGCAT
  GTATCTGCTCTCGGACAAGGCCACCTCGCCGCTCTCGCTGGATGCTGGCCTCGGGCAGGCCCCCTGGAGC
  GACCTGCTTCTTTGGGCACTGTTGCTGAACAGGGCACAGATGGCCATGTACTTCTGGGAGATGGGTTCCA
  ATGCAGTTTCCTCAGCTCTTGGGGCCTGTTTGCTGCTCCGGGTGATGGCACGCCTGGAGCCTGACGCTGA
  GGAGGCAGCACGGAGGAAAGACCTGGCGTTCAAGTTTGAGGGGATGGGCGTTGACCTCTTTGGCGAGTGC
  TATCGCAGCAGTGAGGTGAGGGCTGCCCGCCTCCTCCTCCGTCGCTGCCCGCTCTGGGGGGATGCCACTT
  GCCTCCAGCTGGCCATGCAAGCTGACGCCCGTGCCTTCTTTGCCCAGGATGGGGTACAGTCTCTGCTGAC
  ACAGAAGTGGTGGGGAGATATGGCCAGCACTACACCCATCTGGGCCCTGGTTCTCGCCTTCTTTTGCCCT
  CCACTCATCTACACCCGCCTCATCACCTTCAGGAAATCAGAAGAGGAGCCCACACGGGAGGAGCTAGAGT
  TTGACATGGATAGTGTCATTAATGGGGAAGGGCCTGTCGGGACGGCGGACCCAGCCGAGAAGACGCCGCT
  GGGGGTCCCGCGCCAGTCGGGCCGTCCGGGTTGCTGCGGGGGCCGCTGCGGGGGGCGCCGGTGCCTACGC
  CGCTGGTTCCACTTCTGGGGCGCGCCGGTGACCATCTTCATGGGCAACGTGGTCAGCTACCTGCTGTTCC
  TGCTGCTTTTCTCGCGGGTGCTGCTCGTGGATTTCCAGCCGGCGCCGCCCGGCTCCCTGGAGCTGCTGCT
  CTATTTCTGGGCTTTCACGCTGCTGTGCGAGGAACTGCGCCAGGGCCTGAGCGGAGGCGGGGGCAGCCTC
  GCCAGCGGGGGCCCCGGGCCTGGCCATGCCTCACTGAGCCAGCGCCTGCGCCTCTACCTCGCCGACAGCT
  GGAACCAGTGCGACCTAGTGGCTCTCACCTGCTTCCTCCTGGGCGTGGGCTGCCGGCTGACCCCGGGTTT
  GTACCACCTGGGCCGCACTGTCCTCTGCATCGACTTCATGGTTTTCACGGTGCGGCTGCTTCACATCTTC
  ACGGTCAACAAACAGCTGGGGCCCAAGATCGTCATCGTGAGCAAGATGATGAAGGACGTGTTCTTCTTCC
  TCTTCTTCCTCGGCGTGTGGCTGGTAGCCTATGGCGTGGCCACGGAGGGGCTCCTGAGGCCACGGGACAG
  TGACTTCCCAAGTATCCTGCGCCGCGTCTTCTACCGTCCCTACCTGCAGATCTTCGGGCAGATTCCCCAG
  GAGGACATGGACGTGGCCCTCATGGAGCACAGCAACTGCTCGTCGGAGCCCGGCTTCTGGGCACACCCTC
  CTGGGGCCCAGGCGGGCACCTGCGTCTCCCAGTATGCCAACTGGCTGGTGGTGCTGCTCCTCGTCATCTT
  CCTGCTCGTGGCCAACATCCTGCTGGTCAACTTGCTCATTGCCATGTTCAGTTACACATTCGGCAAAGTA
  CAGGGCAACAGCGATCTCTACTGGAAGGCGCAGCGTTACCGCCTCATCCGGGAATTCCACTCTCGGCCCG
  CGCTGGCCCCGCCCTTTATCGTCATCTCCCACTTGCGCCTCCTGCTCAGGCAATTGTGCAGGCGACCCCG
  GAGCCCCCAGCCGTCCTCCCCGGCCCTCGAGCATTTCCGGGTTTACCTTTCTAAGGAAGCCGAGCGGAAG
  CTGCTAACGTGGGAATCGGTGCATAAGGAGAACTTTCTGCTGGCACGCGCTAGGGACAAGCGGGAGAGCG
  ACTCCGAGCGTCTGAAGCGCACGTCCCAGAAGGTGGACTTGGCACTGAAACAGCTGGGACACATCCGCGA
  GTACGAACAGCGCCTGAAAGTGCTGGAGCGGGAGGTCCAGCAGTGTAGCCGCGTCCTGGGGTGGGTGGCC
  GAGGCCCTGAGCCGCTCTGCCTTGCTGCCCCCAGGTGGGCCGCCACCCCCTGACCTGCCTGGGTCCAAAG
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216888 representing NM_017636
  Red=Cloning site Green=Tags(s)
  
  MVVPEKEQSWIPKIFKKKTCTTFIVDSTDPGGTLCQCGRPRTAHPAVAMEDAFGAAVVTVWDSDAHTTEK
  PTDAYGELDFTGAGRKHSNFLRLSDRTDPAAVYSLVTRTWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRR
  GLVRAAQSTGAWIVTGGLHTGIGRHVGVAVRDHQMASTGGTKVVAMGVAPWGVVRNRDTLINPKGSFPAR
  YRWRGDPEDGVQFPLDYNYSAFFLVDDGTHGCLGGENRFRLRLESYISQQKTGVGGTGIDIPVLLLLIDG
  DEKMLTRIENATQAQLPCLLVAGSGGAADCLAETLEDTLAPGSGGARQGEARDRIRRFFPKGDLEVLQAQ
  VERIMTRKELLTVYSSEDGSEEFETIVLKALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQW
  RSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPMRLAQLYSAAPSNSLIRNLLDQASHSAGTKAPA
  LKGGAAELRPPDVGHVLRMLLGKMCAPRYPSGGAWDPHPGQGFGESMYLLSDKATSPLSLDAGLGQAPWS
  DLLLWALLLNRAQMAMYFWEMGSNAVSSALGACLLLRVMARLEPDAEEAARRKDLAFKFEGMGVDLFGEC
  YRSSEVRAARLLLRRCPLWGDATCLQLAMQADARAFFAQDGVQSLLTQKWWGDMASTTPIWALVLAFFCP
  PLIYTRLITFRKSEEEPTREELEFDMDSVINGEGPVGTADPAEKTPLGVPRQSGRPGCCGGRCGGRRCLR
  RWFHFWGAPVTIFMGNVVSYLLFLLLFSRVLLVDFQPAPPGSLELLLYFWAFTLLCEELRQGLSGGGGSL
  ASGGPGPGHASLSQRLRLYLADSWNQCDLVALTCFLLGVGCRLTPGLYHLGRTVLCIDFMVFTVRLLHIF
  TVNKQLGPKIVIVSKMMKDVFFFLFFLGVWLVAYGVATEGLLRPRDSDFPSILRRVFYRPYLQIFGQIPQ
  EDMDVALMEHSNCSSEPGFWAHPPGAQAGTCVSQYANWLVVLLLVIFLLVANILLVNLLIAMFSYTFGKV
  QGNSDLYWKAQRYRLIREFHSRPALAPPFIVISHLRLLLRQLCRRPRSPQPSSPALEHFRVYLSKEAERK
  LLTWESVHKENFLLARARDKRESDSERLKRTSQKVDLALKQLGHIREYEQRLKVLEREVQQCSRVLGWVA
  EALSRSALLPPGGPPPPDLPGSKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017636
• ORF Size: 3642 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017636.4
• RefSeq Size: 4061 bp
• RefSeq ORF: 3645 bp
• Locus ID: 54795
• UniProt ID: Q8TD43
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 134.1 kDa
• Gene Summary: The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. [provided by RefSeq, Mar 2016]