APRT (NM_000485) Human Tagged ORF Clone

CAT#: RC216874L4

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  • LentiORF®

Lenti ORF clone of Human adenine phosphoribosyltransferase (APRT), transcript variant 1, mGFP tagged



  "NM_000485" in other vectors (4)

USD 640.00


Availability*
3 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name APRT (NM_000485) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag mGFP
Symbol APRT
Synonyms AMP; APRTD
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RC216874).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NM_000485
ORF Size 540 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000485.2
RefSeq Size 807 bp
RefSeq ORF 543 bp
Locus ID 353
Cytogenetics 16q24.3
Domains Pribosyltran
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Purine metabolism
MW 19.6 kDa
Gene Summary Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Other Versions

Other products for "APRT"
Frequently bought together (3)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

USD 590.00


Lenti ORF control particles of pLenti-C-mGFP-P2A-Puro, >1x10^7 TU/ml, 0.5 ml
    • 500 ul

USD 310.00


Rabbit polyclonal APRT Antibody (N-term)
    • 400 ul

USD 465.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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