NEMF (NM_004713) Human Tagged ORF Clone

CAT#: RC216806

NEMF (Myc-DDK-tagged)-Human nuclear export mediator factor (NEMF)

Product Images

Circular map for RC216806

• 

  Western blot validation of overexpression lysate (Cat# LY417803) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216806 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NEMF
• Synonyms: IDDSAPN; NY-CO-1; SDCCAG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216806 representing NM_004713
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGCCGCTTTAGCACCATTGACCTCCGCGCCGTACTCGCGGAGCTGAATGCTAGCTTGCTAGGAA
  TGAGAGTAAACAATGTTTATGATGTGGATAATAAGACATACCTTATTCGTCTTCAAAAACCGGACTTTAA
  AGCTACACTTTTACTTGAATCTGGCATACGAATTCATACAACAGAATTTGAGTGGCCTAAGAATATGATG
  CCGTCTAGTTTTGCCATGAAGTGCCGAAAACATTTGAAGAGTCGGAGATTAGTCAGTGCAAAACAGCTTG
  GTGTGGATAGAATTGTAGATTTTCAATTTGGAAGTGATGAAGCTGCTTACCATTTAATCATTGAGCTCTA
  TGATAGGGGGAACATTGTTCTTACAGATTATGAGTACGTAATTTTAAATATTCTAAGGTTTCGAACTGAT
  GAGGCAGATGATGTTAAATTTGCTGTTCGTGAACGCTATCCACTTGATCATGCTAGAGCTGCTGAACCTT
  TGCTTACTTTGGAAAGGTTGACTGAAATAGTAGCCAGCGCACCTAAGGGTGAACTACTGAAGAGGGTGCT
  TAACCCATTACTTCCCTATGGACCAGCTCTCATTGAACACTGTCTTTTAGAAAATGGATTCTCGGGTAAT
  GTCAAAGTGGATGAAAAACTTGAAACTAAAGATATTGAAAAAGTACTTGTTTCTCTGCAGAAAGCAGAAG
  ACTATATGAAAACAACATCCAACTTCAGTGGGAAGGGATATATCATTCAGAAAAGAGAAATAAAACCATG
  CTTGGAAGCAGATAAACCAGTTGAAGACATACTGACGTATGAGGAATTTCATCCTTTCTTGTTTTCTCAA
  CATTCACAATGTCCATATATAGAATTTGAATCATTTGACAAGGCGGTGGATGAATTTTATTCCAAGATAG
  AAGGCCAGAAAATTGACTTAAAAGCTTTACAACAGGAAAAGCAAGCATTGAAGAAATTAGATAATGTTCG
  AAAGGATCACGAAAACAGATTGGAAGCTCTTCAGCAGGCTCAGGAAATAGACAAACTGAAAGGAGAGCTC
  ATAGAAATGAACCTACAAATAGTTGACAGAGCCATTCAGGTAGTTCGAAGTGCTTTAGCTAACCAGATAG
  ATTGGACAGAAATTGGGTTAATTGTGAAAGAAGCCCAGGCTCAAGGAGACCCTGTTGCAAGTGCAATCAA
  AGAATTAAAACTACAAACAAACCATGTTACAATGCTGCTAAGAAATCCATACTTGTTATCAGAGGAGGAA
  GATGATGATGTTGATGGTGACGTCAATGTTGAGAAAAATGAAACTGAACCACCAAAAGGAAAAAAGAAAA
  AACAAAAGAATAAACAGCTGCAGAAGCCTCAGAAAAATAAGCCCTTACTTGTAGATGTTGATCTCAGCTT
  GTCAGCATATGCCAATGCCAAAAAGTATTATGATCACAAGAGATATGCTGCTAAGAAAACACAAAAGACT
  GTTGAAGCTGCTGAGAAGGCATTCAAGTCAGCAGAAAAGAAAACAAAGCAAACATTAAAAGAAGTTCAGA
  CTGTTACCTCTATTCAAAAAGCAAGAAAAGTATATTGGTTTGAGAAATTTCTGTGGTTCATTAGCTCAGA
  GAACTATCTAATTATAGGTGGACGAGATCAGCAACAGAATGAAATAATTGTGAAAAGATACTTGACACCA
  GGAGACATTTATGTACATGCTGATCTTCATGGAGCTACTAGCTGTGTAATTAAGAATCCAACAGGAGAAC
  CCATCCCCCCACGGACCTTGACTGAAGCTGGCACAATGGCACTTTGCTACAGTGCTGCTTGGGATGCACG
  AGTTATCACTAGTGCTTGGTGGGTGTACCATCATCAGGTATCTAAAACAGCACCAACTGGAGAATATTTG
  ACAACAGGAAGCTTCATGATAAGAGGAAAAAAGAATTTTCTTCCTCCCTCATATCTAATGATGGGGTTTA
  GCTTCCTTTTTAAGGTAGATGAGTCTTGTGTTTGGAGACATCAGGGTGAACGAAAAGTCAGAGTACAGGA
  TGAAGACATGGAGACACTGGCAAGTTGTACAAGTGAACTCATATCAGAAGAAATGGAACAATTAGATGGA
  GGTGACACGAGCAGTGATGAGGATAAAGAAGAACATGAAACTCCTGTGGAAGTAGAACTCATGACTCAGG
  TTGACCAAGAGGATATCACTCTTCAGAGTGGCAGAGATGAACTAAATGAGGAGCTCATTCAGGAAGAAAG
  CTCTGAAGACGAAGGAGAATATGAAGAGGTTAGAAAAGATCAGGATTCTGTTGGTGAAATGAAGGATGAA
  GGGGAAGAGACATTAAATTATCCTGATACTACCATTGACTTGTCTCACCTTCAACCCCAAAGGTCCATCC
  AGAAATTGGCTTCAAAAGAGGAATCTTCTAATTCTAGTGACAGTAAATCACAGAGCCGGAGACATTTGTC
  AGCCAAGGAAAGAAGGGAAATGAAAAAGAAAAAACTTCCAAGTGACTCAGGAGATTTAGAAGCGTTAGAG
  GGAAAGGATAAAGAAAAAGAAAGTACTGTACACATTGAAACTCATCAGAACACAAGCAAAAATGTTGCGG
  CTGTGCAGCCAATGAAACGAGGACAAAAGAGTAAAATGAAAAAAATGAAAGAAAAATACAAAGACCAGGA
  TGAAGAAGACCGTGAACTTATCATGAAGTTGCTGGGGTCTGCAGGTTCAAACAAAGAAGAAAAAGGGAAG
  AAGGGGAAGAAAGGAAAAACAAAGGACGAACCTGTGAAGAAACAGCCCCAGAAACCTAGAGGTGGACAGA
  GGGTCTCTGACAACATTAAGAAAGAAACTCCGTTCCTTGAGGTTATAACTCATGAGTTACAAGACTTTGC
  TGTAGATGATCCACATGATGACAAGGAAGAGCAAGATCTGGATCAACAGGGAAATGAGGAAAACCTATTT
  GATTCTTTGACAGGCCAGCCACATCCTGAAGATGTACTACTGTTTGCCATTCCAATATGTGCCCCTTACA
  CCACCATGACAAACTACAAATATAAAGTGAAACTTACTCCTGGAGTGCAGAAAAAGGGAAAAGCTGCAAA
  AACAGCCTTGAATAGTTTCATGCATTCCAAAGAAGCAACAGCAAGAGAAAAAGACTTATTCCGCAGCGTA
  AAGGACACAGATTTATCAAGAAACATTCCTGGCAAAGTGAAAGTGTCTGCACCCAATCTTCTGAACGTAA
  AAAGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216806 representing NM_004713
  Red=Cloning site Green=Tags(s)
  
  MKSRFSTIDLRAVLAELNASLLGMRVNNVYDVDNKTYLIRLQKPDFKATLLLESGIRIHTTEFEWPKNMM
  PSSFAMKCRKHLKSRRLVSAKQLGVDRIVDFQFGSDEAAYHLIIELYDRGNIVLTDYEYVILNILRFRTD
  EADDVKFAVRERYPLDHARAAEPLLTLERLTEIVASAPKGELLKRVLNPLLPYGPALIEHCLLENGFSGN
  VKVDEKLETKDIEKVLVSLQKAEDYMKTTSNFSGKGYIIQKREIKPCLEADKPVEDILTYEEFHPFLFSQ
  HSQCPYIEFESFDKAVDEFYSKIEGQKIDLKALQQEKQALKKLDNVRKDHENRLEALQQAQEIDKLKGEL
  IEMNLQIVDRAIQVVRSALANQIDWTEIGLIVKEAQAQGDPVASAIKELKLQTNHVTMLLRNPYLLSEEE
  DDDVDGDVNVEKNETEPPKGKKKKQKNKQLQKPQKNKPLLVDVDLSLSAYANAKKYYDHKRYAAKKTQKT
  VEAAEKAFKSAEKKTKQTLKEVQTVTSIQKARKVYWFEKFLWFISSENYLIIGGRDQQQNEIIVKRYLTP
  GDIYVHADLHGATSCVIKNPTGEPIPPRTLTEAGTMALCYSAAWDARVITSAWWVYHHQVSKTAPTGEYL
  TTGSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHQGERKVRVQDEDMETLASCTSELISEEMEQLDG
  GDTSSDEDKEEHETPVEVELMTQVDQEDITLQSGRDELNEELIQEESSEDEGEYEEVRKDQDSVGEMKDE
  GEETLNYPDTTIDLSHLQPQRSIQKLASKEESSNSSDSKSQSRRHLSAKERREMKKKKLPSDSGDLEALE
  GKDKEKESTVHIETHQNTSKNVAAVQPMKRGQKSKMKKMKEKYKDQDEEDRELIMKLLGSAGSNKEEKGK
  KGKKGKTKDEPVKKQPQKPRGGQRVSDNIKKETPFLEVITHELQDFAVDDPHDDKEEQDLDQQGNEENLF
  DSLTGQPHPEDVLLFAIPICAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNSFMHSKEATAREKDLFRSV
  KDTDLSRNIPGKVKVSAPNLLNVKRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004713
• ORF Size: 3228 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004713.5, NP_004704.2
• RefSeq Size: 4136 bp
• RefSeq ORF: 3231 bp
• Locus ID: 9147
• UniProt ID: O60524
• Cytogenetics: 14q21.3
• MW: 122.8 kDa
• Gene Summary: This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016]