OR2A25 (NM_001004488) Human Tagged ORF Clone

CAT#: RC216800

OR2A25 (Myc-DDK-tagged)-Human olfactory receptor, family 2, subfamily A, member 25 (OR2A25)

Product Images

Circular map for RC216800

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  Western blot validation of overexpression lysate (Cat# LY423867) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216800 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR2A25
• Synonyms: OR2A24P; OR2A25P; OR2A27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216800 representing NM_001004488
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGGAAATCAGACTTCCATCACAGAGTTCCTCCTACTGGGATTTCCCATTGGCCCAAGGATTCAGA
  TGCTCCTCTTTGGGCTCTTCTCCCTGTTCTACATCTTCATTCTGTTGGGGAACGGGACAATCCTGGGGCT
  CATCTCACTGGACTCCAGACTCCACACCCCCATGTACTTCTTCCTCTCACACCTGGCGGTCGTCGACATC
  GCCTGTGCTTGCAGCACGGTGCCCCAGATGCTGGTGAACCTCCTGCATCCAGCCAAGCCCATCTCCTTTG
  CTGGCTGCATGACCCAGATGTTTCTGTTTTTGAGTTTTGCACATACAGAATGTCTCCTCCTGGTGGTGAT
  GTCCTATGATCGGTACGTGGCCATCTGCCACCCTCTCCGATATTCTACCATCATGACCTGGAAAGTCTGC
  ATCACTTTGGCATTGACTTCCTGGATTTTAGGAGTCTTATTGGCCCTTGTCCATCTAGTGTTACTGCTAC
  CACTGTCCTTCTGTGGACCCCAGAAACTTAATCACTTTTTCTGTGAAATTATGGCTGTTCTCAAACTTGC
  CTGTGCGGATACCCACATTAATGAGGTAATGGTTTTGGCAGGGGCAGTGTCTGTGCTGGTGGGAGCCTTC
  TTTTCCACTGTAATATCTTATGTTCATATTCTATGTGCCATTCTAAAGATCCAGTCAGGAGAGGGGTGCC
  AGAAAGCCTTCTCCATCTGCTCCTCCCACCTCTGTGTGGTTGGACTCTTTTATGGCACAGCCATCATCAT
  GTATGTTGAGCCCCAGTATGAGAGCCCCAAGGAGCAGAAGAAATATCTCCTGCTGTTTCACAGCCTCTTC
  AATCCCATGCTTAATCCCCTAATTTATAGTCTTAGGAACAAGGAAGTCCAAGGTACTCTAAAGAGGATGC
  TTGAAAAGAAGAGAACTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216800 representing NM_001004488
  Red=Cloning site Green=Tags(s)
  
  MGGNQTSITEFLLLGFPIGPRIQMLLFGLFSLFYIFILLGNGTILGLISLDSRLHTPMYFFLSHLAVVDI
  ACACSTVPQMLVNLLHPAKPISFAGCMTQMFLFLSFAHTECLLLVVMSYDRYVAICHPLRYSTIMTWKVC
  ITLALTSWILGVLLALVHLVLLLPLSFCGPQKLNHFFCEIMAVLKLACADTHINEVMVLAGAVSVLVGAF
  FSTVISYVHILCAILKIQSGEGCQKAFSICSSHLCVVGLFYGTAIIMYVEPQYESPKEQKKYLLLFHSLF
  NPMLNPLIYSLRNKEVQGTLKRMLEKKRTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004488
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004488.1, NP_001004488.1
• RefSeq Size: 933 bp
• RefSeq ORF: 933 bp
• Locus ID: 392138
• UniProt ID: A4D2G3
• Cytogenetics: 7q35
• Protein Pathways: Olfactory transduction
• MW: 34.4 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]