TAT (NM_000353) Human Tagged ORF Clone
Lenti ORF clone of Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein, mGFP tagged
|Product Name||TAT (NM_000353) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||1362 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2757 bp|
|RefSeq ORF||1365 bp|
|UniProt ID||P17735, A0A140VKB7|
|Protein Families||Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS|
|Protein Pathways||Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism, Ubiquinone and other terpenoid-quinone biosynthesis|
|Gene Summary||This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible cognitive disability. A regulator gene for tyrosine aminotransferase is X-linked. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RG216782||TAT (GFP-tagged) - Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein||
|RC216782L3||Lenti ORF clone of Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged||
|RC216782||TAT (Myc-DDK-tagged)-Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein||
|SC119924||TAT (untagged)-Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein||