NAIP (NM_004536) Human Tagged ORF Clone

CAT#: RC216758

NAIP (Myc-DDK-tagged)-Human NLR family, apoptosis inhibitory protein (NAIP), transcript variant 1

Product Images

Circular map for RC216758

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NAIP
• Synonyms: BIRC1; NLRB1; psiNAIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216758 representing NM_004536
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCCAGCAGAAAGCCTCTGACGAGAGGATCTCCCAGTTTGATCACAATTTGCTGCCAGAGCTGT
  CTGCTCTTCTGGGCCTAGATGCAGTTCAGTTGGCAAAGGAACTAGAAGAAGAGGAGCAGAAGGAGCGAGC
  AAAAATGCAGAAAGGCTACAACTCTCAAATGCGCAGTGAAGCAAAAAGGTTAAAGACTTTTGTGACTTAT
  GAGCCGTACAGCTCATGGATACCACAGGAGATGGCGGCCGCTGGGTTTTACTTCACTGGGGTAAAATCTG
  GGATTCAGTGCTTCTGCTGTAGCCTAATCCTCTTTGGTGCCGGCCTCACGAGACTCCCCATAGAAGACCA
  CAAGAGGTTTCATCCAGATTGTGGGTTCCTTTTGAACAAGGATGTTGGTAACATTGCCAAGTACGACATA
  AGGGTGAAGAATCTGAAGAGCAGGCTGAGAGGAGGTAAAATGAGGTACCAAGAAGAGGAGGCTAGACTTG
  CATCCTTCAGGAACTGGCCATTTTATGTCCAAGGGATATCCCCTTGTGTGCTCTCAGAGGCTGGCTTTGT
  CTTTACAGGTAAACAGGACACGGTACAGTGTTTTTCCTGTGGTGGATGTTTAGGAAATTGGGAAGAAGGA
  GATGATCCTTGGAAGGAACATGCCAAATGGTTCCCCAAATGTGAATTTCTTCGGAGTAAGAAATCCTCAG
  AGGAAATTACCCAGTATATTCAAAGCTACAAGGGATTTGTTGACATAACGGGAGAACATTTTGTGAATTC
  CTGGGTCCAGAGAGAATTACCTATGGCATCAGCTTATTGCAATGACAGCATCTTTGCTTACGAAGAACTA
  CGGCTGGACTCTTTTAAGGACTGGCCCCGGGAATCAGCTGTGGGAGTTGCAGCACTGGCCAAAGCAGGTC
  TTTTCTACACAGGTATAAAGGACATCGTCCAGTGCTTTTCCTGTGGAGGGTGTTTAGAGAAATGGCAGGA
  AGGTGATGACCCATTAGACGATCACACCAGATGTTTTCCCAATTGTCCATTTCTCCAAAATATGAAGTCC
  TCTGCGGAAGTGACTCCAGACCTTCAGAGCCGTGGTGAACTTTGTGAATTACTGGAAACCACAAGTGAAA
  GCAATCTTGAAGATTCAATAGCAGTTGGTCCTATAGTGCCAGAAATGGCACAGGGTGAAGCCCAGTGGTT
  TCAAGAGGCAAAGAATCTGAATGAGCAGCTGAGAGCAGCTTATACCAGCGCCAGTTTCCGCCACATGTCT
  TTGCTTGATATCTCTTCCGATCTGGCCACGGACCACTTGCTGGGCTGTGATCTGTCTATTGCTTCAAAAC
  ACATCAGCAAACCTGTGCAAGAACCTCTGGTGCTGCCTGAGGTCTTTGGCAACTTGAACTCTGTCATGTG
  TGTGGAGGGTGAAGCTGGAAGTGGAAAGACGGTCCTCCTGAAGAAAATAGCTTTTCTGTGGGCATCTGGA
  TGCTGTCCCCTGTTAAACAGGTTCCAGCTGGTTTTCTACCTCTCCCTTAGTTCCACCAGACCAGACGAGG
  GGCTGGCCAGTATCATCTGTGACCAGCTCCTAGAGAAAGAAGGATCTGTTACTGAAATGTGCATGAGGAA
  CATTATCCAGCAGTTAAAGAATCAGGTCTTATTCCTTTTAGATGACTACAAAGAAATATGTTCAATCCCT
  CAAGTCATAGGAAAACTGATTCAAAAAAACCACTTATCCCGGACCTGCCTATTGATTGCTGTCCGTACAA
  ACAGGGCCAGGGACATCCGCCGATACCTAGAGACCATTCTAGAGATCAAAGCATTTCCCTTTTATAATAC
  TGTCTGTATATTACGGAAGCTCTTTTCACATAATATGACTCGTCTGCGAAAGTTTATGGTTTACTTTGGA
  AAGAACCAAAGTTTGCAGAAGATACAGAAAACTCCTCTCTTTGTGGCGGCGATCTGTGCTCATTGGTTTC
  AGTATCCTTTTGACCCATCCTTTGATGATGTGGCTGTTTTCAAGTCCTATATGGAACGCCTTTCCTTAAG
  GAACAAAGCGACAGCTGAAATTCTCAAAGCAACTGTGTCCTCCTGTGGTGAGCTGGCCTTGAAAGGGTTT
  TTTTCATGTTGCTTTGAGTTTAATGATGATGATCTCGCAGAAGCAGGGGTTGATGAAGATGAAGATCTAA
  CCATGTGCTTGATGAGCAAATTTACAGCCCAGAGACTAAGACCATTCTACCGGTTTTTAAGTCCTGCCTT
  CCAAGAATTTCTTGCGGGGATGAGGCTGATTGAACTCCTGGATTCAGATAGGCAGGAACATCAAGATTTG
  GGACTGTATCATTTGAAACAAATCAACTCACCCATGATGACTGTAAGCGCCTACAACAATTTTTTGAACT
  ATGTCTCCAGCCTCCCTTCAACAAAAGCAGGGCCCAAAATTGTGTCTCATTTGCTCCATTTAGTGGATAA
  CAAAGAGTCATTGGAGAATATATCTGAAAATGATGACTACTTAAAGCACCAGCCAGAAATTTCACTGCAG
  ATGCAGTTACTTAGGGGATTGTGGCAAATTTGTCCACAAGCTTACTTTTCAATGGTTTCAGAACATTTAC
  TGGTTCTTGCCCTGAAAACTGCTTATCAAAGCAACACTGTTGCTGCGTGTTCTCCATTTGTTTTGCAATT
  CCTTCAAGGGAGAACACTGACTTTGGGTGCGCTTAACTTACAGTACTTTTTCGACCACCCAGAAAGCTTG
  TCATTGTTGAGGAGCATCCACTTCCCAATACGAGGAAATAAGACATCACCCAGAGCACATTTTTCAGTTC
  TGGAAACATGTTTTGACAAATCACAGGTGCCAACTATAGATCAGGACTATGCTTCTGCCTTTGAACCTAT
  GAATGAATGGGAGCGAAATTTAGCTGAAAAAGAGGATAATGTAAAGAGCTATATGGATATGCAGCGCAGG
  GCATCACCAGACCTTAGTACTGGCTATTGGAAACTTTCTCCAAAGCAGTACAAGATTCCCTGTCTAGAAG
  TCGATGTGAATGATATTGATGTTGTAGGCCAGGATATGCTTGAGATTCTAATGACAGTTTTCTCAGCTTC
  ACAGCGCATCGAACTCCATTTAAACCACAGCAGAGGCTTTATAGAAAGCATCCGCCCAGCTCTTGAGCTG
  TCTAAGGCCTCTGTCACCAAGTGCTCCATAAGCAAGTTGGAACTCAGCGCAGCCGAACAGGAACTGCTTC
  TCACCCTGCCTTCCCTGGAATCTCTTGAAGTCTCAGGGACAATCCAGTCACAAGACCAAATCTTTCCTAA
  TCTGGATAAGTTCCTGTGCCTGAAAGAACTGTCTGTGGATCTGGAGGGCAATATAAATGTTTTTTCAGTC
  ATTCCTGAAGAATTTCCAAACTTCCACCATATGGAGAAATTATTGATCCAAATTTCAGCTGAGTATGATC
  CTTCCAAACTAGTAAAATTAATTCAAAATTCTCCAAACCTTCATGTTTTCCATCTGAAGTGTAACTTCTT
  TTCGGATTTTGGGTCTCTCATGACTATGCTTGTTTCCTGTAAGAAACTCACAGAAATTAAGTTTTCGGAT
  TCATTTTTTCAAGCCGTCCCATTTGTTGCCAGTTTGCCAAATTTTATTTCTCTGAAGATATTAAATCTTG
  AAGGCCAGCAATTTCCTGATGAGGAAACATCAGAAAAATTTGCCTACATTTTAGGTTCTCTTAGTAACCT
  GGAAGAATTGATCCTTCCTACTGGGGATGGAATTTATCGAGTGGCCAAACTGATCATCCAGCAGTGTCAG
  CAGCTTCATTGTCTCCGAGTCCTCTCATTTTTCAAGACTTTGAATGATGACAGCGTGGTGGAAATTGCCA
  AAGTAGCAATCAGTGGAGGTTTCCAGAAACTTGAGAACCTAAAGCTTTCAATCAATCACAAGATTACAGA
  GGAAGGATACAGAAATTTCTTTCAAGCACTGGACAACATGCCAAACTTGCAGGAGTTGGACATCTCCAGG
  CATTTCACAGAGTGTATCAAAGCTCAGGCCACAACAGTCAAGTCTTTGAGTCAATGTGTGTTACGACTAC
  CAAGGCTCATTAGACTGAACATGTTAAGTTGGCTCTTGGATGCAGATGATATTGCATTGCTTAATGTCAT
  GAAAGAAAGACATCCTCAATCTAAGTACTTAACTATTCTCCAGAAATGGATACTGCCGTTCTCTCCAATC
  ATTCAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216758 representing NM_004536
  Red=Cloning site Green=Tags(s)
  
  MATQQKASDERISQFDHNLLPELSALLGLDAVQLAKELEEEEQKERAKMQKGYNSQMRSEAKRLKTFVTY
  EPYSSWIPQEMAAAGFYFTGVKSGIQCFCCSLILFGAGLTRLPIEDHKRFHPDCGFLLNKDVGNIAKYDI
  RVKNLKSRLRGGKMRYQEEEARLASFRNWPFYVQGISPCVLSEAGFVFTGKQDTVQCFSCGGCLGNWEEG
  DDPWKEHAKWFPKCEFLRSKKSSEEITQYIQSYKGFVDITGEHFVNSWVQRELPMASAYCNDSIFAYEEL
  RLDSFKDWPRESAVGVAALAKAGLFYTGIKDIVQCFSCGGCLEKWQEGDDPLDDHTRCFPNCPFLQNMKS
  SAEVTPDLQSRGELCELLETTSESNLEDSIAVGPIVPEMAQGEAQWFQEAKNLNEQLRAAYTSASFRHMS
  LLDISSDLATDHLLGCDLSIASKHISKPVQEPLVLPEVFGNLNSVMCVEGEAGSGKTVLLKKIAFLWASG
  CCPLLNRFQLVFYLSLSSTRPDEGLASIICDQLLEKEGSVTEMCMRNIIQQLKNQVLFLLDDYKEICSIP
  QVIGKLIQKNHLSRTCLLIAVRTNRARDIRRYLETILEIKAFPFYNTVCILRKLFSHNMTRLRKFMVYFG
  KNQSLQKIQKTPLFVAAICAHWFQYPFDPSFDDVAVFKSYMERLSLRNKATAEILKATVSSCGELALKGF
  FSCCFEFNDDDLAEAGVDEDEDLTMCLMSKFTAQRLRPFYRFLSPAFQEFLAGMRLIELLDSDRQEHQDL
  GLYHLKQINSPMMTVSAYNNFLNYVSSLPSTKAGPKIVSHLLHLVDNKESLENISENDDYLKHQPEISLQ
  MQLLRGLWQICPQAYFSMVSEHLLVLALKTAYQSNTVAACSPFVLQFLQGRTLTLGALNLQYFFDHPESL
  SLLRSIHFPIRGNKTSPRAHFSVLETCFDKSQVPTIDQDYASAFEPMNEWERNLAEKEDNVKSYMDMQRR
  ASPDLSTGYWKLSPKQYKIPCLEVDVNDIDVVGQDMLEILMTVFSASQRIELHLNHSRGFIESIRPALEL
  SKASVTKCSISKLELSAAEQELLLTLPSLESLEVSGTIQSQDQIFPNLDKFLCLKELSVDLEGNINVFSV
  IPEEFPNFHHMEKLLIQISAEYDPSKLVKLIQNSPNLHVFHLKCNFFSDFGSLMTMLVSCKKLTEIKFSD
  SFFQAVPFVASLPNFISLKILNLEGQQFPDEETSEKFAYILGSLSNLEELILPTGDGIYRVAKLIIQQCQ
  QLHCLRVLSFFKTLNDDSVVEIAKVAISGGFQKLENLKLSINHKITEEGYRNFFQALDNMPNLQELDISR
  HFTECIKAQATTVKSLSQCVLRLPRLIRLNMLSWLLDADDIALLNVMKERHPQSKYLTILQKWILPFSPI
  IQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004536
• ORF Size: 4209 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004536.3
• RefSeq Size: 6133 bp
• RefSeq ORF: 4212 bp
• Locus ID: 4671
• UniProt ID: Q13075
• Cytogenetics: 5q13.2
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 159.4 kDa
• Gene Summary: This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This copy of the gene is full length; additional copies with truncations and internal deletions are also present in this region of chromosome 5q13. It is thought that this gene is a modifier of spinal muscular atrophy caused by mutations in a neighboring gene, SMN1. The protein encoded by this gene contains regions of homology to two baculovirus inhibitor of apoptosis proteins, and it is able to suppress apoptosis induced by various signals. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Nov 2016]