WDHD1 (NM_007086) Human Tagged ORF Clone

CAT#: RC216745

WDHD1 (Myc-DDK-tagged)-Human WD repeat and HMG-box DNA binding protein 1 (WDHD1), transcript variant 1

Product Images

Circular map for RC216745

• 

  Coomassie blue staining of purified WDHD1 protein (Cat# TP316745). The protein was produced from HEK293T cells transfected with WDHD1 cDNA clone (Cat# RC216745) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDHD1
• Synonyms: AND-1; AND1; CHTF4; CTF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216745 representing NM_007086
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGCCACACGGAAGCCAATGAGATATGGGCATACAGAGGGACACACGGAGGTCTGTTTTGATGATT
  CTGGGAGTTTTATTGTGACTTGTGGAAGTGATGGTGATGTGAGGATTTGGGAAGACTTGGATGATGATGA
  TCCTAAGTTCATTAATGTTGGAGAAAAGGCATATTCATGTGCTTTGAAGAGTGGAAAACTGGTCACTGCA
  GTTTCTAATAATACTATTCAAGTCCACACATTTCCTGAAGGAGTTCCAGATGGTATATTGACTCGCTTCA
  CTACAAATGCAAACCATGTGGTCTTTAATGGGGATGGTACTAAAATTGCTGCTGGATCTAGTGATTTTCT
  AGTCAAAATTGTGGATGTGATGGATAGCAGCCAACAGAAAACATTTCGAGGACATGATGCCCCTGTTTTA
  AGTCTTTCCTTTGATCCTAAGGACATCTTTCTGGCATCAGCTAGTTGTGATGGATCTGTCAGAGTGTGGC
  AAATTTCAGATCAGACATGTGCTATTAGTTGGCCACTGCTACAAAAATGCAACGATGTGATAAATGCAAA
  ATCAATCTGCAGACTTGCTTGGCAGCCAAAAAGTGGGAAGTTACTGGCAATTCCTGTGGAAAAATCTGTT
  AAGCTATATAGAAGAGAATCTTGGAGTCATCAATTTGATCTTTCAGATAATTTCATCTCTCAGACCCTCA
  ATATAGTAACCTGGTCTCCCTGTGGGCAATATTTAGCTGCAGGTAGTATTAATGGTCTAATCATAGTTTG
  GAATGTGGAAACCAAAGACTGCATGGAAAGGGTGAAACATGAGAAAGGTTATGCAATTTGTGGTCTGGCA
  TGGCATCCTACTTGTGGTCGAATATCGTATACTGATGCGGAAGGAAATCTAGGGCTTCTAGAGAATGTTT
  GTGACCCCAGTGGAAAGACATCAAGCAGTAAGGTATCTAGCAGAGTGGAAAAGGATTATAATGATCTTTT
  TGATGGAGATGATATGAGTAATGCTGGTGATTTTCTAAATGACAATGCAGTTGAGATCCCTTCTTTTTCA
  AAAGGGATTATAAATGATGATGAGGATGATGAAGACCTCATGATGGCTTCAGGTCGTCCTAGACAGCGAA
  GTCACATCCTAGAAGATGATGAAAACTCAGTTGATATTTCAATGCTAAAAACTGGTTCTAGTCTTCTCAA
  AGAGGAGGAGGAAGATGGTCAAGAAGGCAGCATTCACAATCTACCACTTGTAACATCCCAAAGGCCATTT
  TATGATGGACCCATGCCAACTCCCCGGCAAAAGCCATTTCAGTCAGGTTCTACACCGTTGCATCTCACTC
  ACAGATTCATGGTGTGGAACTCTATTGGAATTATTCGCTGCTATAATGATGAGCAAGACAATGCCATAGA
  TGTGGAGTTCCATGATACCTCCATACACCATGCAACACACTTATCAAACACTTTGAATTATACAATAGCA
  GATCTTTCCCACGAAGCTATTTTGTTGGCATGTGAAAGCACTGATGAACTAGCAAGCAAGCTTCACTGCC
  TGCACTTTAGTTCTTGGGATTCAAGCAAAGAGTGGATAATAGACTTGCCTCAGAATGAGGATATTGAAGC
  CATATGTCTCGGTCAAGGATGGGCTGCTGCCGCTACTAGTGCCCTGCTTCTTCGATTGTTTACTATTGGA
  GGGGTTCAAAAAGAGGTATTCAGCCTTGCTGGACCTGTGGTGTCAATGGCAGGACATGGAGAACAGCTTT
  TCATTGTTTATCACAGAGGTACAGGATTTGATGGGGATCAGTGCCTTGGAGTTCAACTGCTAGAGCTGGG
  GAAAAAGAAAAAACAAATTTTGCATGGTGACCCTCTTCCTCTTACAAGGAAATCCTACCTTGCATGGATT
  GGGTTTTCAGCTGAAGGTACCCCTTGTTACGTGGATTCAGAAGGAATTGTTCGAATGCTTAACAGAGGAC
  TTGGTAATACGTGGACTCCTATATGTAATACAAGAGAGCACTGCAAAGGAAAATCTGATCACTACTGGGT
  GGTTGGTATCCATGAAAATCCCCAGCAACTAAGGTGCATTCCTTGTAAAGGTTCTCGGTTTCCCCCAACC
  CTTCCACGCCCTGCTGTTGCTATATTATCCTTTAAGCTTCCTTACTGTCAGATTGCAACAGAGAAAGGAC
  AAATGGAGGAGCAATTTTGGCGTTCAGTTATATTTCACAACCACCTTGATTATTTAGCTAAAAATGGTTA
  TGAATATGAAGAGAGCACTAAAAATCAAGCAACAAAAGAGCAACAGGAACTTTTAATGAAAATGCTTGCG
  CTTTCTTGTAAACTGGAGCGAGAATTCCGTTGTGTGGAACTTGCTGATCTAATGACTCAAAATGCTGTGA
  ATTTAGCCATTAAATATGCTTCTCGCTCTCGGAAATTAATACTGGCTCAAAAACTAAGTGAACTGGCTGT
  AGAGAAGGCAGCCGAATTGACAGCAACCCAGGTGGAAGAGGAAGAAGAAGAAGAAGATTTCAGAAAAAAG
  CTGAATGCTGGTTACAGCAATACTGCTACAGAGTGGAGCCAACCAAGGTTCAGAAATCAAGTTGAAGAAG
  ATGCTGAGGACAGTGGAGAAGCTGATGATGAAGAAAAACCAGAAATACATAAGCCTGGACAGAACTCGTT
  TTCCAAAAGTACAAATTCCTCTGATGTTTCAGCTAAGTCAGGTGCAGTTACCTTTAGCAGCCAAGGACGA
  GTAAATCCCTTTAAGGTATCAGCCAGTTCCAAAGAACCAGCCATGTCAATGAATTCAGCACGTTCAACTA
  ATATTTTAGACAATATGGGCAAATCATCCAAGAAATCCACTGCACTTAGTCGAACTACAAATAATGAAAA
  GTCTCCCATTATAAAGCCTCTGATTCCAAAGCCGAAGCCTAAGCAGGCATCTGCAGCATCCTATTTCCAG
  AAAAGAAATTCTCAAACTAATAAAACTGAGGAAGTGAAAGAAGAAAATCTTAAAAATGTATTATCTGAAA
  CCCCAGCTATATGTCCTCCTCAAAACACTGAAAACCAAAGGCCAAAGACCGGGTTCCAGATGTGGTTAGA
  AGAAAATAGAAGTAATATTTTGTCTGACAATCCTGACTTTTCAGATGAAGCAGACATAATAAAAGAAGGA
  ATGATTCGATTTAGAGTATTGTCAACTGAAGAAAGAAAGGTGTGGGCTAACAAAGCCAAAGGAGAAACGG
  CAAGTGAAGGAACTGAAGCAAAGAAGCGAAAACGTGTGGTTGATGAAAGTGATGAAACAGAAAACCAGGA
  AGAAAAAGCAAAAGAGAACCTGAATTTGTCTAAAAAGCAGAAACCTTTAGATTTTTCTACAAATCAGAAA
  CTATCAGCTTTTGCATTTAAGCAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216745 representing NM_007086
  Red=Cloning site Green=Tags(s)
  
  MPATRKPMRYGHTEGHTEVCFDDSGSFIVTCGSDGDVRIWEDLDDDDPKFINVGEKAYSCALKSGKLVTA
  VSNNTIQVHTFPEGVPDGILTRFTTNANHVVFNGDGTKIAAGSSDFLVKIVDVMDSSQQKTFRGHDAPVL
  SLSFDPKDIFLASASCDGSVRVWQISDQTCAISWPLLQKCNDVINAKSICRLAWQPKSGKLLAIPVEKSV
  KLYRRESWSHQFDLSDNFISQTLNIVTWSPCGQYLAAGSINGLIIVWNVETKDCMERVKHEKGYAICGLA
  WHPTCGRISYTDAEGNLGLLENVCDPSGKTSSSKVSSRVEKDYNDLFDGDDMSNAGDFLNDNAVEIPSFS
  KGIINDDEDDEDLMMASGRPRQRSHILEDDENSVDISMLKTGSSLLKEEEEDGQEGSIHNLPLVTSQRPF
  YDGPMPTPRQKPFQSGSTPLHLTHRFMVWNSIGIIRCYNDEQDNAIDVEFHDTSIHHATHLSNTLNYTIA
  DLSHEAILLACESTDELASKLHCLHFSSWDSSKEWIIDLPQNEDIEAICLGQGWAAAATSALLLRLFTIG
  GVQKEVFSLAGPVVSMAGHGEQLFIVYHRGTGFDGDQCLGVQLLELGKKKKQILHGDPLPLTRKSYLAWI
  GFSAEGTPCYVDSEGIVRMLNRGLGNTWTPICNTREHCKGKSDHYWVVGIHENPQQLRCIPCKGSRFPPT
  LPRPAVAILSFKLPYCQIATEKGQMEEQFWRSVIFHNHLDYLAKNGYEYEESTKNQATKEQQELLMKMLA
  LSCKLEREFRCVELADLMTQNAVNLAIKYASRSRKLILAQKLSELAVEKAAELTATQVEEEEEEEDFRKK
  LNAGYSNTATEWSQPRFRNQVEEDAEDSGEADDEEKPEIHKPGQNSFSKSTNSSDVSAKSGAVTFSSQGR
  VNPFKVSASSKEPAMSMNSARSTNILDNMGKSSKKSTALSRTTNNEKSPIIKPLIPKPKPKQASAASYFQ
  KRNSQTNKTEEVKEENLKNVLSETPAICPPQNTENQRPKTGFQMWLEENRSNILSDNPDFSDEADIIKEG
  MIRFRVLSTEERKVWANKAKGETASEGTEAKKRKRVVDESDETENQEEKAKENLNLSKKQKPLDFSTNQK
  LSAFAFKQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007086
• ORF Size: 3387 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007086.4
• RefSeq Size: 4734 bp
• RefSeq ORF: 3390 bp
• Locus ID: 11169
• UniProt ID: O75717
• Cytogenetics: 14q22.2-q22.3
• Domains: HMG, WD40
• Protein Families: Druggable Genome, Transcription Factors
• MW: 125.8 kDa
• Gene Summary: The protein encoded by this gene contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]