ASB3 (NM_016115) Human Tagged ORF Clone

CAT#: RC216743

ASB3 (Myc-DDK-tagged)-Human ankyrin repeat and SOCS box containing 3 (ASB3), transcript variant 1

Product Images

Circular map for RC216743

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  Western blot validation of overexpression lysate (Cat# LY402502) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216743 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ASB3 protein (Cat# TP316743). The protein was produced from HEK293T cells transfected with ASB3 cDNA clone (Cat# RC216743) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASB3
• Synonyms: ASB-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216743 representing NM_016115
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTTACAGAGGCTTACGCGGACACGTGCTCTACAGTTGGACTTGCTGCCAGGGAAGGCAATGTTA
  AAGTCTTAAGGAAACTGCTCAAAAAGGGCCGAAGTGTCGATGTTGCTGATAACAGGGGATGGATGCCAAT
  TCATGAAGCAGCTTATCACAACTCTGTAGAATGTTTGCAAATGTTAATTAATGCAGATTCATCTGAAAAC
  TACATTAAGATGAAGACCTTTGAAGGTTTCTGTGCTTTGCATCTCGCTGCAAGTCAAGGACATTGGAAAA
  TCGTACAGATTCTTTTAGAAGCTGGGGCAGATCCTAATGCAACTACTTTAGAAGAAACGACACCATTGTT
  TTTAGCTGTTGAAAATGGACAGATAGATGTGTTAAGGCTGTTGCTTCAACACGGAGCAAATGTTAATGGA
  TCCCATTCTATGTGTGGATGGAACTCCTTGCACCAGGCTTCTTTTCAGGAAAATGCTGAGATCATAAAAT
  TGCTTCTTAGAAAAGGAGCAAACAAGGAATGCCAGGATGACTTTGGAATCACACCTTTATTTGTGGCTGC
  TCAGTATGGCAAGCTAGAAAGCTTGAGCATACTTATTTCATCGGGTGCAAATGTCAATTGTCAAGCCTTG
  GACAAAGCTACACCCTTGTTCATTGCTGCTCAAGAGGGACACACAAAATGTGTGGAGCTTTTGCTCTCCA
  GTGGGGCAGATCCTGATCTTTACTGTAATGAGGACAGTTGGCAGTTACCTATTCATGCAGCTGCACAAAT
  GGGCCATACAAAAATCTTGGACTTGTTAATACCACTTACTAACCGGGCCTGTGACACTGGGCTAAACAAA
  GTAAGCCCTGTTTACTCAGCAGTGTTTGGGGGACATGAAGATTGCCTAGAAATATTACTCCGGAATGGCT
  ACAGCCCAGACGCCCAGGCGTGCCTTGTTTTTGGATTCAGTTCTCCTGTGTGCATGGCTTTCCAAAAGGA
  CTGTGAGTTCTTTGGAATTGTGAACATTCTTTTGAAATATGGAGCCCAGATAAATGAACTTCATTTGGCA
  TACTGCCTGAAGTACGAGAAGTTTTCGATATTTCGCTACTTTTTGAGGAAAGGTTGCTCATTGGGACCAT
  GGAACCATATATATGAATTTGTAAATCATGCAATTAAAGCACAAGCAAAATATAAGGAGTGGTTGCCACA
  TCTTCTGGTTGCTGGATTTGACCCACTGATTCTACTGTGCAATTCTTGGATTGACTCAGTCAGCATTGAC
  ACCCTTATCTTCACTTTGGAGTTTACTAATTGGAAGACACTTGCACCAGCTGTTGAAAGGATGCTCTCTG
  CTCGTGCCTCAAACGCTTGGATTCTACAGCAACATATTGCCACTGTTCCATCCCTGACCCATCTTTGTCG
  TTTGGAAATTCGGTCCAGTCTAAAATCAGAACGTCTACGGTCTGACAGTTATATTAGTCAGCTGCCACTT
  CCCAGAAGCCTACATAATTATTTGCTCTATGAAGACGTTCTGAGGATGTATGAAGTTCCAGAACTGGCAG
  CTATTCAAGATGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216743 representing NM_016115
  Red=Cloning site Green=Tags(s)
  
  MDFTEAYADTCSTVGLAAREGNVKVLRKLLKKGRSVDVADNRGWMPIHEAAYHNSVECLQMLINADSSEN
  YIKMKTFEGFCALHLAASQGHWKIVQILLEAGADPNATTLEETTPLFLAVENGQIDVLRLLLQHGANVNG
  SHSMCGWNSLHQASFQENAEIIKLLLRKGANKECQDDFGITPLFVAAQYGKLESLSILISSGANVNCQAL
  DKATPLFIAAQEGHTKCVELLLSSGADPDLYCNEDSWQLPIHAAAQMGHTKILDLLIPLTNRACDTGLNK
  VSPVYSAVFGGHEDCLEILLRNGYSPDAQACLVFGFSSPVCMAFQKDCEFFGIVNILLKYGAQINELHLA
  YCLKYEKFSIFRYFLRKGCSLGPWNHIYEFVNHAIKAQAKYKEWLPHLLVAGFDPLILLCNSWIDSVSID
  TLIFTLEFTNWKTLAPAVERMLSARASNAWILQQHIATVPSLTHLCRLEIRSSLKSERLRSDSYISQLPL
  PRSLHNYLLYEDVLRMYEVPELAAIQDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016115
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016115.5
• RefSeq Size: 2214 bp
• RefSeq ORF: 1557 bp
• Locus ID: 51130
• UniProt ID: Q9Y575
• Cytogenetics: 2p16.2
• Domains: ANK
• Protein Families: Druggable Genome
• MW: 57.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2011]