LARP4 (NM_052879) Human Tagged ORF Clone

CAT#: RC216731

LARP4 (Myc-DDK-tagged)-Human La ribonucleoprotein domain family, member 4 (LARP4), transcript variant 1

Product Images

Circular map for RC216731

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LARP4
• Synonyms: PP13296
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216731 representing NM_052879
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCTTTTCGTGGAGCAGGTAGCATCTAAAGGAACTGGTTTAAATCCTAATGCCAAAGTATGGCAAG
  AAATTGCTCCTGGAAATACTGATGCCACCCCAGTAACTCATGGAACTGAAAGCTCTTGGCATGAAATAGC
  AGCTACATCAGGTGCTCATCCTGAGGGTAATGCAGAGCTCTCAGAAGATATATGTAAAGAATATGAAGTA
  ATGTATTCTTCATCTTGTGAAACCACAAGAAATACTACAGGCATTGAAGAATCAACTGATGGGATGATTT
  TAGGACCAGAAGATCTGAGTTACCAAATATATGATGTTTCCGGAGAAAGCAATTCAGCAGTTTCTACAGA
  AGACCTAAAAGAATGTCTGAAGAAACAATTAGAATTCTGTTTTTCACGAGAAAATTTGTCAAAGGATCTT
  TACTTGATATCTCAAATGGATAGTGATCAGTTCATCCCAATTTGGACAGTTGCCAACATGGAAGAAATAA
  AAAAGTTGACTACAGACCCTGATCTAATTCTTGAAGTGTTAAGATCTTCTCCCATGGTACAAGTTGATGA
  GAAGGGTGAGAAAGTGAGACCAAGTCATAAGCGTTGTATTGTAATTCTTAGAGAGATTCCTGAAACAACA
  CCAATAGAGGAAGTGAAAGGTTTGTTCAAAAGTGAAAACTGCCCCAAAGTGATAAGCTGTGAGTTTGCAC
  ACAATAGCAACTGGTATATCACTTTCCAGTCAGACACAGATGCACAACAGGCTTTTAAATACTTAAGAGA
  AGAAGTTAAAACATTTCAGGGCAAGCCAATTATGGCAAGGATAAAAGCCATCAATACATTTTTTGCTAAG
  AATGGTTATCGATTAATGGATTCTAGTATCTATAGTCACCCCATTCAAACTCAAGCACAGTATGCCTCCC
  CAGTCTTTATGCAGCCTGTATATAATCCTCACCAACAGTACTCGGTCTATAGTATTGTGCCTCAGTCTTG
  GTCTCCAAATCCTACACCTTACTTTGAAACACCACTGGCTCCCTTTCCCAATGGTAGTTTTGTGAATGGC
  TTTAATTCGCCAGGATCTTATAAAACAAATGCTGCTGCTATGAATATGGGTCGACCATTCCAAAAAAATC
  GTGTGAAGCCTCAGTTTAGGTCATCTGGTGGTTCAGAACACTCAACAGAGGGCTCTGTATCCTTGGGGGA
  TGGACAGTTGAACAGATATAGTTCAAGAAACTTTCCAGCTGAACGGCATAACCCCACAGTAACTGGGCAT
  CAGGAGCAAACTTACCTTCAGAAGGAGACTTCCACTTTGCAGGTGGAACAGAATGGGGACTATGGTAGGG
  GCAGGAGAACTCTCTTCAGAGGTCGAAGACGACGAGAAGATGACAGGATCTCAAGACCTCATCCTTCAAC
  AGCTGAATCAAAGGCTCCAACACCAAAGTTTGACTTATTAGCCTCAAATTTTCCACCTTTACCTGGAAGT
  TCATCAAGAATGCCAGGTGAACTCGTTTTGGAGAATAGGATGTCTGATGTTGTTAAAGGTGTCTACAAAG
  AAAAGGATAATGAAGAGTTGACAATTAGTTGCCCAGTGCCTGCAGATGAGCAGACAGAATGCACTTCTGC
  CCAGCAACTCAATATGAGTACCAGTTCTCCATGTGCTGCTGAGCTTACTGCATTAAGCACAACTCAGCAA
  GAAAAGGATCTAATAGAAGATTCCTCTGTTCAGAAGGATGGTCTCAATCAGACAACTATACCAGTTTCTC
  CTCCAAGTACTACAAAGCCATCGAGGGCAAGTACTGCTTCACCATGTAATAATAACATAAATGCAGCTAC
  AGCTGTGGCTCTACAGGAACCCCGAAAGTTAAGTTATGCTGAAGTGTGCCAGAAGCCCCCTAAAGAGCCA
  TCTTCAGTTCTTGTGCAGCCACTACGGGAACTTCGCTCCAATGTGGTGTCTCCCACCAAAAATGAAGACA
  ATGGAGCTCCTGAGAACTCCGTTGAGAAACCACATGAGAAGCCAGAAGCAAGGGCTAGTAAGGATTATTC
  TGGCTTCCGAGGCAATATAATCCCCAGGGGAGCAGCAGGAAAAATCAGGGAACAGAGACGCCAGTTTAGC
  CATAGGGCTATACCTCAGGGAGTGACTCGACGTAATGGCAAAGAGCAATATGTGCCACCCAGATCACCAA
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216731 representing NM_052879
  Red=Cloning site Green=Tags(s)
  
  MLLFVEQVASKGTGLNPNAKVWQEIAPGNTDATPVTHGTESSWHEIAATSGAHPEGNAELSEDICKEYEV
  MYSSSCETTRNTTGIEESTDGMILGPEDLSYQIYDVSGESNSAVSTEDLKECLKKQLEFCFSRENLSKDL
  YLISQMDSDQFIPIWTVANMEEIKKLTTDPDLILEVLRSSPMVQVDEKGEKVRPSHKRCIVILREIPETT
  PIEEVKGLFKSENCPKVISCEFAHNSNWYITFQSDTDAQQAFKYLREEVKTFQGKPIMARIKAINTFFAK
  NGYRLMDSSIYSHPIQTQAQYASPVFMQPVYNPHQQYSVYSIVPQSWSPNPTPYFETPLAPFPNGSFVNG
  FNSPGSYKTNAAAMNMGRPFQKNRVKPQFRSSGGSEHSTEGSVSLGDGQLNRYSSRNFPAERHNPTVTGH
  QEQTYLQKETSTLQVEQNGDYGRGRRTLFRGRRRREDDRISRPHPSTAESKAPTPKFDLLASNFPPLPGS
  SSRMPGELVLENRMSDVVKGVYKEKDNEELTISCPVPADEQTECTSAQQLNMSTSSPCAAELTALSTTQQ
  EKDLIEDSSVQKDGLNQTTIPVSPPSTTKPSRASTASPCNNNINAATAVALQEPRKLSYAEVCQKPPKEP
  SSVLVQPLRELRSNVVSPTKNEDNGAPENSVEKPHEKPEARASKDYSGFRGNIIPRGAAGKIREQRRQFS
  HRAIPQGVTRRNGKEQYVPPRSPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052879
• ORF Size: 2172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052879.5
• RefSeq Size: 6537 bp
• RefSeq ORF: 2175 bp
• Locus ID: 113251
• UniProt ID: Q71RC2
• Cytogenetics: 12q13.12
• Protein Families: Transcription Factors
• MW: 80.6 kDa
• Gene Summary: RNA binding protein that binds to the poly-A tract of mRNA molecules (PubMed:21098120). Associates with the 40S ribosomal subunit and with polysomes (PubMed:21098120). Plays a role in the regulation of mRNA translation (PubMed:21098120). Plays a role in the regulation of cell morphology and cytoskeletal organization (PubMed:21834987, PubMed:27615744).[UniProtKB/Swiss-Prot Function]