IKK alpha (CHUK) (NM_001278) Human Tagged ORF Clone

CAT#: RC216718

CHUK (Myc-DDK-tagged)-Human conserved helix-loop-helix ubiquitous kinase (CHUK)

Product Images

Circular map for RC216718

• 

  Western blot validation of overexpression lysate (Cat# LY400513) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216718 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IKK alpha
• Synonyms: BPS2; IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216718 representing NM_001278
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCCCTGGGAGATGCGGGAGCGGCTGGGCACCG
  GCGGCTTCGGGAACGTCTGTCTGTACCAGCATCGGGAACTTGATCTCAAAATAGCAATTAAGTCTTGTCG
  CCTAGAGCTAAGTACCAAAAACAGAGAACGATGGTGCCATGAAATCCAGATTATGAAGAAGTTGAACCAT
  GCCAATGTTGTAAAGGCCTGTGATGTTCCTGAAGAATTGAATATTTTGATTCATGATGTGCCTCTTCTAG
  CAATGGAATACTGTTCTGGAGGAGATCTCCGAAAGCTGCTCAACAAACCAGAAAATTGTTGTGGACTTAA
  AGAAAGCCAGATACTTTCTTTACTAAGTGATATAGGGTCTGGGATTCGATATTTGCATGAAAACAAAATT
  ATACATCGAGATCTAAAACCTGAAAACATAGTTCTTCAGGATGTTGGTGGAAAGATAATACATAAAATAA
  TTGATCTGGGATATGCCAAAGATGTTGATCAAGGAAGTCTGTGTACATCTTTTGTGGGAACACTGCAGTA
  TCTGGCCCCAGAGCTCTTTGAGAATAAGCCTTACACAGCCACTGTTGATTATTGGAGCTTTGGGACCATG
  GTATTTGAATGTATTGCTGGATATAGGCTTTTTTTGCATCATCTGCAGCCATTTACCTGGCATGAGAAGA
  TTAAGAAGAAGGATCCAAAGTGTATATTTGCATGTGAAGAGATGTCAGGAGAAGTTCGGTTTAGTAGCCA
  TTTACCTCAACCAAATAGCCTTTGTAGTTTAATAGTAGAACCCATGGAAAACTGGCTACAGTTGATGTTG
  AATTGGGACCCTCAGCAGAGAGGAGGACCTGTTGACCTTACTTTGAAGCAGCCAAGATGTTTTGTATTAA
  TGGATCACATTTTGAATTTGAAGATAGTACACATCCTAAATATGACTTCTGCAAAGATAATTTCTTTTCT
  GTTACCACCTGATGAAAGTCTTCATTCACTACAGTCTCGTATTGAGCGTGAAACTGGAATAAATACTGGT
  TCTCAAGAACTTCTTTCAGAGACAGGAATTTCTCTGGATCCTCGGAAACCAGCCTCTCAATGTGTTCTAG
  ATGGAGTTAGAGGCTGTGATAGCTATATGGTTTATTTGTTTGATAAAAGTAAAACTGTATATGAAGGGCC
  ATTTGCTTCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAGGACAGCAAAATACAGCTTCCAATT
  ATACAGCTGCGTAAAGTGTGGGCTGAAGCAGTGCACTATGTGTCTGGACTAAAAGAAGACTATAGCAGGC
  TCTTTCAGGGACAAAGGGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTAACAAAAATGAAGAA
  CACTTTGATCTCAGCATCACAACAACTGAAAGCTAAATTGGAGTTTTTTCACAAAAGCATTCAGCTTGAC
  TTGGAGAGATACAGCGAGCAGATGACGTATGGGATATCTTCAGAAAAAATGCTAAAAGCATGGAAAGAAA
  TGGAAGAAAAGGCCATCCACTATGCTGAGGTTGGTGTCATTGGATACCTGGAGGATCAGATTATGTCTTT
  GCATGCTGAAATCATGGAGCTACAGAAGAGCCCCTATGGAAGACGTCAGGGAGACTTGATGGAATCTCTG
  GAACAGCGTGCCATTGATCTATATAAGCAGTTAAAACACAGACCTTCAGATCACTCCTACAGTGACAGCA
  CAGAGATGGTGAAAATCATTGTGCACACTGTGCAGAGTCAGGACCGTGTGCTCAAGGAGCTGTTTGGTCA
  TTTGAGCAAGTTGTTGGGCTGTAAGCAGAAGATTATTGATCTACTCCCTAAGGTGGAAGTGGCCCTCAGT
  AATATCAAAGAAGCTGACAATACTGTCATGTTCATGCAGGGAAAAAGGCAGAAAGAAATATGGCATCTCC
  TTAAAATTGCCTGTACACAGAGTTCTGCCCGGTCCCTTGTAGGATCCAGTCTAGAAGGTGCAGTAACCCC
  TCAGACATCAGCATGGCTGCCCCCGACTTCAGCAGAACATGATCATTCTCTGTCATGTGTGGTAACTCCT
  CAAGATGGGGAGACTTCAGCACAAATGATAGAAGAAAATTTGAACTGCCTTGGCCATTTAAGCACTATTA
  TTCATGAGGCAAATGAGGAACAGGGCAATAGTATGATGAATCTTGATTGGAGTTGGTTAACAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216718 representing NM_001278
  Red=Cloning site Green=Tags(s)
  
  MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNH
  ANVVKACDVPEELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKI
  IHRDLKPENIVLQDVGGKIIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTM
  VFECIAGYRLFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLIVEPMENWLQLML
  NWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTG
  SQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPI
  IQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLD
  LERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESL
  EQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALS
  NIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTP
  QDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278.5
• RefSeq Size: 3539 bp
• RefSeq ORF: 2238 bp
• Locus ID: 1147
• UniProt ID: O15111
• Cytogenetics: 10q24.31
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway
• MW: 84.5 kDa
• Gene Summary: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]