SERINC4 (NM_001033517) Human Tagged ORF Clone

CAT#: RC216546

SERINC4 (Myc-DDK-tagged)-Human serine incorporator 4 (SERINC4)

Product Images

Western blot validation of overexpression lysate (Cat# LY422376) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216546 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SERINC4
• Synonyms: FLJ40363
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216546 representing NM_001033517
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTATCTCTTCCCAGCATGGCATTACATTGGCATCTGTGGAGGCTTTGCATTCATCCTACTGCAGTT
  GGTGCTTATTACAGCTTTTGCCCATTCCTGGAACAAGAACTGAGCAACCCCGCTCTGGCCTCCTACAAGC
  TTCTGTCATCAGCTGCTATATCATGTATCTGACTTTCTCTGCACTGTCCAGCCGTCCTCCAGAGAGAGTA
  ATCCTTCAAGGACAGAATCACACCCTGTGCCTGCCTGGCCTGAGTAAAATGGAACCCCAAACACCAGATA
  TCTCTCTAGCAATGCTGAGTGCTAGCATCATGTATGCTTGTGTGCTTTTTGCTTGCAATGAGGCCTCCTA
  CCTGGCTGAGGTATTTGGACCCCTGTGGATTGTCAAGGTTTACAGCTATGAGTTTCAGAAGCCCTCACTG
  TGTTTCTGCTGCCCTGAAACAGTGGAGGCAGACAAAGGGCAAAGGGGTGGGGCTGCGAGGCCAGCTGACC
  AAGAAACCCCTCCAGCTCCTCCAGTCCAAGTCCAGCATCTTTCCTACAACTATTCTGCCTTCCACTTCGT
  CTTCTTCCTTGCCTCACTCTATGTCATGGTTACCCTTACCAACTGGTTCAGCTATGAGGGAGCAGAACTG
  GAAAAGACCTTCATCAAGGGTAGCTGGGCCACCTTCTGGGTCAAGGTTGCCTCATGCTGGGCCTGCGTAC
  TCCTCTATCTGGGGCTGTTACTGGCACCACTCTGTTGGCCCCCCACCCAGAAACCCCAGCCCCTTATCTT
  GAGGCGCCGCCGCCACCGCATCATATCCCCAGATAACAAATATCCTCCAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216546 representing NM_001033517
  Red=Cloning site Green=Tags(s)
  
  MSISSQHGITLASVEALHSSYCSWCLLQLLPIPGTRTEQPRSGLLQASVISCYIMYLTFSALSSRPPERV
  ILQGQNHTLCLPGLSKMEPQTPDISLAMLSASIMYACVLFACNEASYLAEVFGPLWIVKVYSYEFQKPSL
  CFCCPETVEADKGQRGGAARPADQETPPAPPVQVQHLSYNYSAFHFVFFLASLYVMVTLTNWFSYEGAEL
  EKTFIKGSWATFWVKVASCWACVLLYLGLLLAPLCWPPTQKPQPLILRRRRHRIISPDNKYPPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033517
• ORF Size: 822 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033517.1, NP_001028689.1
• RefSeq Size: 1490 bp
• RefSeq ORF: 824 bp
• Locus ID: 619189
• Cytogenetics: 15q15.3
• Protein Families: Transmembrane
• MW: 30.5 kDa
• Gene Summary: Incorporates a polar amino acid serine into membranes and facilitates the synthesis of two serine-derived lipids, phosphatidylserine and sphingolipids.[UniProtKB/Swiss-Prot Function]