GABA A Receptor alpha 5 (GABRA5) (NM_000810) Human Tagged ORF Clone

CAT#: RC216541

GABRA5 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5), transcript variant 1

Product Images

Circular map for RC216541

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GABRA5 (Cat# RC216541, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GABRA5(Cat# TA806473). Positive lysates LY400284 (100ug) and LC400284 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY431938) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228910 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GABRA5 protein (Cat# TP316541). The protein was produced from HEK293T cells transfected with GABRA5 cDNA clone (Cat# RC216541) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GABA A Receptor alpha 5
• Synonyms: DEE79; EIEE79
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216541 representing NM_000810.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACAATGGAATGTTCTCTGGTTTTATCATGATCAAAAACCTCCTTCTCTTTTGTATTTCCATGAAC
  TTATCCAGTCACTTTGGCTTTTCACAGATGCCAACCAGTTCAGTGAAAGATGAGACCAATGACAACATC
  ACGATATTTACCAGGATCTTGGATGGGCTCTTGGATGGCTACGACAACAGACTTCGGCCCGGGCTGGGA
  GAGCGCATCACTCAGGTGAGGACCGACATCTACGTCACCAGCTTCGGCCCGGTGTCCGACACGGAAATG
  GAGTACACCATAGACGTGTTTTTCCGACAAAGCTGGAAAGATGAAAGGCTTCGGTTTAAGGGGCCCATG
  CAGCGCCTCCCTCTCAACAACCTCCTTGCCAGCAAGATCTGGACCCCAGACACGTTCTTCCACAACGGG
  AAGAAGTCCATCGCTCACAACATGACCACGCCCAACAAGCTGCTGCGGCTGGAGGACGACGGCACCCTG
  CTCTACACCATGCGCTTGACCATCTCTGCAGAGTGCCCCATGCAGCTTGAGGACTTCCCGATGGATGCG
  CACGCTTGCCCTCTGAAATTTGGCAGCTATGCGTACCCTAATTCTGAAGTCGTTTACGTCTGGACCAAC
  GGCTCCACCAAGTCGGTGGTGGTGGCGGAAGATGGCTCCAGACTGAACCAGTACCACCTGATGGGGCAG
  ACGGTGGGCACTGAGAACATCAGCACCAGCACAGGCGAATACACAATCATGACAGCTCACTTCCACCTG
  AAAAGGAAGATTGGCTACTTTGTCATCCAGACCTACCTTCCCTGCATAATGACCGTGATCTTATCACAG
  GTGTCCTTTTGGCTGAACCGGGAATCAGTCCCAGCCAGGACAGTTTTTGGGGTCACCACGGTGCTGACC
  ATGACGACCCTCAGCATCAGCGCCAGGAACTCTCTGCCCAAAGTGGCCTACGCCACCGCCATGGACTGG
  TTCATAGCCGTGTGCTATGCCTTCGTCTTCTCGGCGCTGATAGAGTTTGCCACGGTCAATTACTTTACC
  AAGAGAGGCTGGGCCTGGGATGGCAAAAAAGCCTTGGAAGCAGCCAAGATCAAGAAAAAGCGTGAAGTC
  ATACTAAATAAGTCAACAAACGCTTTTACAACTGGGAAGATGTCTCACCCCCCAAACATTCCGAAGGAA
  CAGACCCCAGCAGGGACGTCGAATACAACCTCAGTCTCAGTAAAACCCTCTGAAGAGAAGACTTCTGAA
  AGCAAAAAGACTTACAACAGTATCAGCAAAATTGACAAAATGTCCCGAATCGTATTCCCAGTCTTGTTC
  GGCACTTTCAACTTAGTTTACTGGGCAACGTATTTGAATAGGGAGCCGGTGATAAAAGGAGCCGCCTCT
  CCAAAA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC216541
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDNGMFSGFIMIKNLLLFCISMNLSSHFGFSQMPTSSVKDETNDNITIFTRILDGLLDGYDNRLRPGLG
  ERITQVRTDIYVTSFGPVSDTEMEYTIDVFFRQSWKDERLRFKGPMQRLPLNNLLASKIWTPDTFFHNG
  KKSIAHNMTTPNKLLRLEDDGTLLYTMRLTISAECPMQLEDFPMDAHACPLKFGSYAYPNSEVVYVWTN
  GSTKSVVVAEDGSRLNQYHLMGQTVGTENISTSTGEYTIMTAHFHLKRKIGYFVIQTYLPCIMTVILSQ
  VSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFT
  KRGWAWDGKKALEAAKIKKKREVILNKSTNAFTTGKMSHPPNIPKEQTPAGTSNTTSVSVKPSEEKTSE
  SKKTYNSISKIDKMSRIVFPVLFGTFNLVYWATYLNREPVIKGAASPK
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC216541 also available, TP316541M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000810
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000810.4
• RefSeq Size: 2352 bp
• RefSeq ORF: 1389 bp
• Locus ID: 2558
• UniProt ID: P31644
• Cytogenetics: 15q12
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 52.1 kDa
• Gene Summary: GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. [provided by RefSeq, Jul 2008]