TBX5 (NM_000192) Human Tagged ORF Clone

CAT#: RC216520

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TBX5 (Myc-DDK-tagged)-Human T-box 5 (TBX5), transcript variant 1



  "NM_000192" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TBX5
Synonyms HOS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC216520 representing NM_000192
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGACGCAGACGAGGGCTTTGGCCTGGCGCACACGCCTCTGGAGCCTGACGCAAAAGACCTGCCCT
GCGATTCGAAACCCGAGAGCGCGCTCGGGGCCCCCAGCAAGTCCCCGTCGTCCCCGCAGGCCGCCTTCAC
CCAGCAGGGCATGGAGGGAATCAAAGTGTTTCTCCATGAAAGAGAACTGTGGCTAAAATTCCACGAAGTG
GGCACGGAAATGATCATAACCAAGGCTGGAAGGCGGATGTTTCCCAGTTACAAAGTGAAGGTGACGGGCC
TTAATCCCAAAACGAAGTACATTCTTCTCATGGACATTGTACCTGCCGACGATCACAGATACAAATTCGC
AGATAATAAATGGTCTGTGACGGGCAAAGCTGAGCCCGCCATGCCTGGCCGCCTGTACGTGCACCCAGAC
TCCCCCGCCACCGGGGCGCATTGGATGAGGCAGCTCGTCTCCTTCCAGAAACTCAAGCTCACCAACAACC
ACCTGGACCCATTTGGGCATATTATTCTAAATTCCATGCACAAATACCAGCCTAGATTACACATCGTGAA
AGCGGATGAAAATAATGGATTTGGCTCAAAAAATACAGCGTTCTGCACTCACGTCTTTCCTGAGACTGCG
TTTATAGCAGTGACTTCTTACCAGAACCACAAGATCACGCAATTAAAGATTGAGAATAATCCCTTTGCCA
AAGGATTTCGGGGCAGTGATGACATGGAGCTGCACAGAATGTCAAGAATGCAAAGTAAAGAATATCCCGT
GGTCCCCAGGAGCACCGTGAGGCAAAAAGTGGCCTCCAACCACAGTCCTTTCAGCAGCGAGTCTCGAGCT
CTCTCCACCTCATCCAATTTGGGGTCCCAATACCAGTGTGAGAATGGTGTTTCCGGCCCCTCCCAGGACC
TCCTGCCTCCACCCAACCCATACCCACTGCCCCAGGAGCATAGCCAAATTTACCATTGTACCAAGAGGAA
AGAGGAAGAATGTTCCACCACAGACCATCCCTATAAGAAGCCCTACATGGAGACATCACCCAGTGAAGAA
GATTCCTTCTACCGCTCTAGCTATCCACAGCAGCAGGGCCTGGGTGCCTCCTACAGGACAGAGTCGGCAC
AGCGGCAAGCTTGCATGTATGCCAGCTCTGCGCCCCCCAGCGAGCCTGTGCCCAGCCTAGAGGACATCAG
CTGCAACACGTGGCCAAGCATGCCTTCCTACAGCAGCTGCACCGTCACCACCGTGCAGCCCATGGACAGG
CTACCCTACCAGCACTTCTCCGCTCACTTCACCTCGGGGCCCCTGGTCCCTCGGCTGGCTGGCATGGCCA
ACCATGGCTCCCCACAGCTGGGAGAGGGAATGTTCCAGCACCAGACCTCCGTGGCCCACCAGCCTGTGGT
CAGGCAGTGTGGGCCTCAGACTGGCCTGCAGTCCCCTGGCACCCTTCAGCCCCCTGAGTTCCTCTACTCT
CATGGCGTGCCAAGGACTCTATCCCCTCATCAGTACCACTCTGTGCACGGAGTTGGCATGGTGCCAGAGT
GGAGCGACAATAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC216520 representing NM_000192
Red=Cloning site Green=Tags(s)

MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEV
GTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPD
SPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETA
FIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRA
LSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEE
DSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDR
LPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYS
HGVPRTLSPHQYHSVHGVGMVPEWSDNS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000192
ORF Size 1554 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000192.3, NP_000183.2
RefSeq Size 3921 bp
RefSeq ORF 1557 bp
Locus ID 6910
UniProt ID Q99593
Cytogenetics 12q24.21
Domains T-box
Protein Families Druggable Genome, Transcription Factors
MW 57.5 kDa
Gene Summary This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Other Versions

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