NALP1 (NLRP1) (NM_033004) Human Tagged ORF Clone

CAT#: RC216481

NLRP1 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 1 (NLRP1), transcript variant 1

Product Images

Circular map for RC216481

• 

  Western blot validation of overexpression lysate (Cat# LY409802) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216481 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NLRP1 protein (Cat# TP316481). The protein was produced from HEK293T cells transfected with NLRP1 cDNA clone (Cat# RC216481) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NALP1
• Synonyms: AIADK; CARD7; CIDED; CLR17.1; DEFCAP; DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216481 representing NM_033004
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCGGAGCCTGGGGCCGCCTGGCCTGTTACTTGGAGTTCCTGAAGAAGGAGGAGCTGAAGGAGT
  TCCAGCTTCTGCTCGCCAATAAAGCGCACTCCAGGAGCTCTTCGGGTGAGACACCCGCTCAGCCAGAGAA
  GACGAGTGGCATGGAGGTGGCCTCGTACCTGGTGGCTCAGTATGGGGAGCAGCGGGCCTGGGACCTAGCC
  CTCCATACCTGGGAGCAGATGGGGCTGAGGTCACTGTGCGCCCAAGCCCAGGAAGGGGCAGGCCACTCTC
  CCTCATTCCCCTACAGCCCAAGTGAACCCCACCTGGGGTCTCCCAGCCAACCCACCTCCACCGCAGTGCT
  AATGCCCTGGATCCATGAATTGCCGGCGGGGTGCACCCAGGGCTCAGAGAGAAGGGTTTTGAGACAGCTG
  CCTGACACATCTGGACGCCGCTGGAGAGAAATCTCTGCCTCACTCCTCTACCAAGCTCTTCCAAGCTCCC
  CAGACCATGAGTCTCCAAGCCAGGAGTCACCCAACGCCCCCACATCCACAGCAGTGCTGGGGAGCTGGGG
  ATCCCCACCTCAGCCCAGCCTAGCACCCAGAGAGCAGGAGGCTCCTGGGACCCAATGGCCTCTGGATGAA
  ACGTCAGGAATTTACTACACAGAAATCAGAGAAAGAGAGAGAGAGAAATCAGAGAAAGGCAGGCCCCCAT
  GGGCAGCGGTGGTAGGAACGCCCCCACAGGCGCACACCAGCCTACAGCCCCACCACCACCCATGGGAGCC
  TTCTGTGAGAGAGAGCCTCTGTTCCACATGGCCCTGGAAAAATGAGGATTTTAACCAAAAATTCACACAG
  CTGCTACTTCTACAAAGACCTCACCCCAGAAGCCAAGATCCCCTGGTCAAGAGAAGCTGGCCTGATTATG
  TGGAGGAGAATCGAGGACATTTAATTGAGATCAGAGACTTATTTGGCCCAGGCCTGGATACCCAAGAACC
  TCGCATAGTCATACTGCAGGGGGCTGCTGGAATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGGAAGCC
  TGGGGGAGAGGCCAGCTGTATGGGGACCGCTTCCAGCATGTCTTCTACTTCAGCTGCAGAGAGCTGGCCC
  AGTCCAAGGTGGTGAGTCTCGCTGAGCTCATCGGAAAAGATGGGACAGCCACTCCGGCTCCCATTAGACA
  GATCCTGTCTAGGCCAGAGCGGCTGCTCTTCATCCTCGATGGTGTAGATGAGCCAGGATGGGTCTTGCAG
  GAGCCGAGTTCTGAGCTCTGTCTGCACTGGAGCCAGCCACAGCCGGCGGATGCACTGCTGGGCAGTTTGC
  TGGGGAAAACTATACTTCCCGAGGCATCCTTCCTGATCACGGCTCGGACCACAGCTCTGCAGAACCTCAT
  TCCTTCTTTGGAGCAGGCACGTTGGGTAGAGGTCCTGGGGTTCTCTGAGTCCAGCAGGAAGGAATATTTC
  TACAGATATTTCACAGATGAAAGGCAAGCAATTAGAGCCTTTAGGTTGGTCAAATCAAACAAAGAGCTCT
  GGGCCCTGTGTCTTGTGCCCTGGGTGTCCTGGCTGGCCTGCACTTGCCTGATGCAGCAGATGAAGCGGAA
  GGAAAAACTCACACTGACTTCCAAGACCACCACAACCCTCTGTCTACATTACCTTGCCCAGGCTCTCCAA
  GCTCAGCCATTGGGACCCCAGCTCAGAGACCTCTGCTCTCTGGCTGCTGAGGGCATCTGGCAAAAAAAGA
  CCCTTTTCAGTCCAGATGACCTCAGGAAGCATGGGTTAGATGGGGCCATCATCTCCACCTTCTTGAAGAT
  GGGTATTCTTCAAGAGCACCCCATCCCTCTGAGCTACAGCTTCATTCACCTCTGTTTCCAAGAGTTCTTT
  GCAGCAATGTCCTATGTCTTGGAGGATGAGAAGGGGAGAGGTAAACATTCTAATTGCATCATAGATTTGG
  AAAAGACGCTAGAAGCATATGGAATACATGGCCTGTTTGGGGCATCAACCACACGTTTCCTATTGGGCCT
  GTTAAGTGATGAGGGGGAGAGAGAGATGGAGAACATCTTTCACTGCCGGCTGTCTCAGGGGAGGAACCTG
  ATGCAGTGGGTCCCGTCCCTGCAGCTGCTGCTGCAGCCACACTCTCTGGAGTCCCTCCACTGCTTGTACG
  AGACTCGGAACAAAACGTTCCTGACACAAGTGATGGCCCATTTCGAAGAAATGGGCATGTGTGTAGAAAC
  AGACATGGAGCTCTTAGTGTGCACTTTCTGCATTAAATTCAGCCGCCACGTGAAGAAGCTTCAGCTGATT
  GAGGGCAGGCAGCACAGATCAACATGGAGCCCCACCATGGTAGTCCTGTTCAGGTGGGTCCCAGTCACAG
  ATGCCTATTGGCAGATTCTCTTCTCCGTCCTCAAGGTCACCAGAAACCTGAAGGAGCTGGACCTAAGTGG
  AAACTCGCTGAGCCACTCTGCAGTGAAGAGTCTTTGTAAGACCCTGAGACGCCCTCGCTGCCTCCTGGAG
  ACCCTGCGGTTGGCTGGCTGTGGCCTCACAGCTGAGGACTGCAAGGACCTTGCCTTTGGGCTGAGAGCCA
  ACCAGACCCTGACCGAGCTGGACCTGAGCTTCAATGTGCTCACGGATGCTGGAGCCAAACACCTTTGCCA
  GAGACTGAGACAGCCGAGCTGCAAGCTACAGCGACTGCAGCTGGTCAGCTGTGGCCTCACGTCTGACTGC
  TGCCAGGACCTGGCCTCTGTGCTTAGTGCCAGCCCCAGCCTGAAGGAGCTAGACCTGCAGCAGAACAACC
  TGGATGACGTTGGCGTGCGACTGCTCTGTGAGGGGCTCAGGCATCCTGCCTGCAAACTCATACGCCTGGG
  GCTGGACCAGACAACTCTGAGTGATGAGATGAGGCAGGAACTGAGGGCCCTGGAGCAGGAGAAACCTCAG
  CTGCTCATCTTCAGCAGACGGAAACCAAGTGTGATGACCCCTACTGAGGGCCTGGATACGGGAGAGATGA
  GTAATAGCACATCCTCACTCAAGCGGCAGAGACTCGGATCAGAGAGGGCGGCTTCCCATGTTGCTCAGGC
  TAATCTCAAACTCCTGGACGTGAGCAAGATCTTCCCAATTGCTGAGATTGCAGAGGAAAGCTCCCCAGAG
  GTAGTACCGGTGGAACTCTTGTGCGTGCCTTCTCCTGCCTCTCAAGGGGACCTGCATACGAAGCCTTTGG
  GGACTGACGATGACTTCTGGGGCCCCACGGGGCCTGTGGCTACTGAGGTAGTTGACAAAGAAAAGAACTT
  GTACCGAGTTCACTTCCCTGTAGCTGGCTCCTACCGCTGGCCCAACACGGGTCTCTGCTTTGTGATGAGA
  GAAGCGGTGACCGTTGAGATTGAATTCTGTGTGTGGGACCAGTTCCTGGGTGAGATCAACCCACAGCACA
  GCTGGATGGTGGCAGGGCCTCTGCTGGACATCAAGGCTGAGCCTGGAGCTGTGGAAGCTGTGCACCTCCC
  TCACTTTGTGGCTCTCCAAGGGGGCCATGTGGACACATCCCTGTTCCAAATGGCCCACTTTAAAGAGGAG
  GGGATGCTCCTGGAGAAGCCAGCCAGGGTGGAGCTGCATCACATAGTTCTGGAAAACCCCAGCTTCTCCC
  CCTTGGGAGTCCTCCTGAAAATGATCCATAATGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCT
  TTACCACCGCGTCCATCCTGAGGAAGTCACCTTCCACCTCTACCTGATCCCAAGTGACTGCTCCATTCGG
  AAGGCCATAGATGATCTAGAAATGAAATTCCAGTTTGTGCGAATCCACAAGCCACCCCCGCTGACCCCAC
  TTTATATGGGCTGTCGTTACACTGTGTCTGGGTCTGGTTCAGGGATGCTGGAAATACTCCCCAAGGAACT
  GGAGCTCTGCTATCGAAGCCCTGGAGAAGACCAGCTGTTCTCGGAGTTCTACGTTGGCCACTTGGGATCA
  GGGATCAGGCTGCAAGTGAAAGACAAGAAAGATGAGACTCTGGTGTGGGAGGCCTTGGTGAAACCAGGAG
  ATCTCATGCCTGCAACTACTCTGATCCCTCCAGCCCGCATAGCCGTACCTTCACCTCTGGATGCCCCGCA
  GTTGCTGCACTTTGTGGACCAGTATCGAGAGCAGCTGATAGCCCGAGTGACATCGGTGGAGGTTGTCTTG
  GACAAACTGCATGGACAGGTGCTGAGCCAGGAGCAGTACGAGAGGGTGCTGGCTGAGAACACGAGGCCCA
  GCCAGATGCGGAAGCTGTTCAGCTTGAGCCAGTCCTGGGACCGGAAGTGCAAAGATGGACTCTACCAAGC
  CCTGAAGGAGACCCATCCTCACCTCATTATGGAACTCTGGGAGAAGGGCAGCAAAAAGGGACTCCTGCCA
  CTCAGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216481 representing NM_033004
  Red=Cloning site Green=Tags(s)
  
  MAGGAWGRLACYLEFLKKEELKEFQLLLANKAHSRSSSGETPAQPEKTSGMEVASYLVAQYGEQRAWDLA
  LHTWEQMGLRSLCAQAQEGAGHSPSFPYSPSEPHLGSPSQPTSTAVLMPWIHELPAGCTQGSERRVLRQL
  PDTSGRRWREISASLLYQALPSSPDHESPSQESPNAPTSTAVLGSWGSPPQPSLAPREQEAPGTQWPLDE
  TSGIYYTEIREREREKSEKGRPPWAAVVGTPPQAHTSLQPHHHPWEPSVRESLCSTWPWKNEDFNQKFTQ
  LLLLQRPHPRSQDPLVKRSWPDYVEENRGHLIEIRDLFGPGLDTQEPRIVILQGAAGIGKSTLARQVKEA
  WGRGQLYGDRFQHVFYFSCRELAQSKVVSLAELIGKDGTATPAPIRQILSRPERLLFILDGVDEPGWVLQ
  EPSSELCLHWSQPQPADALLGSLLGKTILPEASFLITARTTALQNLIPSLEQARWVEVLGFSESSRKEYF
  YRYFTDERQAIRAFRLVKSNKELWALCLVPWVSWLACTCLMQQMKRKEKLTLTSKTTTTLCLHYLAQALQ
  AQPLGPQLRDLCSLAAEGIWQKKTLFSPDDLRKHGLDGAIISTFLKMGILQEHPIPLSYSFIHLCFQEFF
  AAMSYVLEDEKGRGKHSNCIIDLEKTLEAYGIHGLFGASTTRFLLGLLSDEGEREMENIFHCRLSQGRNL
  MQWVPSLQLLLQPHSLESLHCLYETRNKTFLTQVMAHFEEMGMCVETDMELLVCTFCIKFSRHVKKLQLI
  EGRQHRSTWSPTMVVLFRWVPVTDAYWQILFSVLKVTRNLKELDLSGNSLSHSAVKSLCKTLRRPRCLLE
  TLRLAGCGLTAEDCKDLAFGLRANQTLTELDLSFNVLTDAGAKHLCQRLRQPSCKLQRLQLVSCGLTSDC
  CQDLASVLSASPSLKELDLQQNNLDDVGVRLLCEGLRHPACKLIRLGLDQTTLSDEMRQELRALEQEKPQ
  LLIFSRRKPSVMTPTEGLDTGEMSNSTSSLKRQRLGSERAASHVAQANLKLLDVSKIFPIAEIAEESSPE
  VVPVELLCVPSPASQGDLHTKPLGTDDDFWGPTGPVATEVVDKEKNLYRVHFPVAGSYRWPNTGLCFVMR
  EAVTVEIEFCVWDQFLGEINPQHSWMVAGPLLDIKAEPGAVEAVHLPHFVALQGGHVDTSLFQMAHFKEE
  GMLLEKPARVELHHIVLENPSFSPLGVLLKMIHNALRFIPVTSVVLLYHRVHPEEVTFHLYLIPSDCSIR
  KAIDDLEMKFQFVRIHKPPPLTPLYMGCRYTVSGSGSGMLEILPKELELCYRSPGEDQLFSEFYVGHLGS
  GIRLQVKDKKDETLVWEALVKPGDLMPATTLIPPARIAVPSPLDAPQLLHFVDQYREQLIARVTSVEVVL
  DKLHGQVLSQEQYERVLAENTRPSQMRKLFSLSQSWDRKCKDGLYQALKETHPHLIMELWEKGSKKGLLP
  LSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033004
• ORF Size: 4419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033004.4
• RefSeq Size: 5617 bp
• RefSeq ORF: 4422 bp
• Locus ID: 22861
• UniProt ID: Q9C000
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 165.7 kDa
• Gene Summary: This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]