GABA A Receptor beta 2 (GABRB2) (NM_000813) Human Tagged ORF Clone

CAT#: RC216424

GABRB2 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, beta 2 (GABRB2), transcript variant 2

Product Images

Circular map for RC216424

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  Western blot validation of overexpression lysate (Cat# LY400287) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216424 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GABA A Receptor beta 2
• Synonyms: DEE92; ICEE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216424 representing NM_000813
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGAGTGCGGAAAAGGGGCTACTTTGGGATTTGGTCCTTCCCCTTAATAATCGCCGCTGTCTGTG
  CGCAGAGTGTCAATGACCCTAGTAATATGTCGCTGGTTAAAGAGACGGTGGATAGACTCCTGAAAGGCTA
  TGACATTCGTCTGAGACCAGATTTTGGAGGTCCCCCCGTGGCTGTGGGGATGAACATTGACATTGCCAGC
  ATCGATATGGTTTCTGAAGTCAATATGGATTATACCTTGACAATGTACTTTCAACAAGCCTGGAGAGATA
  AGAGGCTGTCCTATAATGTAATACCTTTAAACTTGACTCTGGACAACAGAGTGGCAGACCAGCTCTGGGT
  GCCTGATACCTATTTCCTGAACGATAAGAAGTCATTTGTGCACGGAGTGACTGTTAAGAACCGCATGATT
  CGCCTGCATCCTGATGGCACCGTCCTTTATGGACTCAGAATCACAACCACAGCTGCCTGCATGATGGACC
  TAAGGAGGTACCCACTGGATGAACAAAACTGCACCTTGGAAATTGAGAGCTATGGATACACAACTGATGA
  CATTGAGTTTTACTGGCGTGGCGATGATAATGCAGTAACAGGAGTAACGAAAATTGAACTTCCACAGTTC
  TCTATTGTAGATTACAAACTTATCACCAAGAAGGTTGTTTTTTCCACAGGTTCCTATCCCAGGTTATCCC
  TCAGCTTTAAGCTTAAGAGAAACATTGGCTACTTTATCCTGCAAACATACATGCCTTCCATCCTGATTAC
  CATCCTCTCCTGGGTCTCCTTCTGGATTAATTACGATGCTTCAGCTGCAAGGGTGGCATTAGGAATCACA
  ACTGTCCTCACAATGACCACAATCAACACCCACCTCCGGGAAACTCTCCCTAAAATCCCCTATGTGAAGG
  CCATTGACATGTACCTGATGGGGTGCTTTGTCTTCGTTTTCATGGCCCTTCTGGAATATGCCCTAGTCAA
  CTACATCTTCTTTGGGAGGGGGCCCCAACGCCAAAAGAAAGCAGCTGAGAAGGCTGCCAGTGCCAACAAT
  GAGAAGATGCGCCTGGATGTCAACAAGATGGACCCCCATGAGAACATCTTACTGAGCACTCTCGAGATAA
  AAAATGAAATGGCCACATCTGAGGCTGTGATGGGACTTGGAGACCCCAGAAGCACAATGCTAGCCTATGA
  TGCCTCCAGCATCCAGTATCGGAAAGCTGGGTTGCCCAGGCATAGTTTTGGCCGAAATGCTCTGGAACGA
  CATGTGGCGCAAAAGAAAAGTCGCCTGAGGAGACGCGCCTCCCAACTGAAAATCACCATCCCTGACTTGA
  CTGATGTGAATGCCATAGATCGGTGGTCCCGCATATTCTTCCCAGTGGTTTTTTCCTTCTTCAACATCGT
  CTATTGGCTTTATTATGTGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216424 representing NM_000813
  Red=Cloning site Green=Tags(s)
  
  MWRVRKRGYFGIWSFPLIIAAVCAQSVNDPSNMSLVKETVDRLLKGYDIRLRPDFGGPPVAVGMNIDIAS
  IDMVSEVNMDYTLTMYFQQAWRDKRLSYNVIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMI
  RLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGDDNAVTGVTKIELPQF
  SIVDYKLITKKVVFSTGSYPRLSLSFKLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGIT
  TVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFMALLEYALVNYIFFGRGPQRQKKAAEKAASANN
  EKMRLDVNKMDPHENILLSTLEIKNEMATSEAVMGLGDPRSTMLAYDASSIQYRKAGLPRHSFGRNALER
  HVAQKKSRLRRRASQLKITIPDLTDVNAIDRWSRIFFPVVFSFFNIVYWLYYVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000813
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000813.3
• RefSeq Size: 1860 bp
• RefSeq ORF: 1425 bp
• Locus ID: 2561
• UniProt ID: P47870
• Cytogenetics: 5q34
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 54.4 kDa
• Gene Summary: The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 2 subunit. It is mapped to chromosome 5q34 in a cluster comprised of genes encoding alpha 1 and gamma 2 subunits of the GABA A receptor. Alternative splicing of this gene generates 2 transcript variants, differing by a 114 bp insertion. [provided by RefSeq, Jul 2008]