TMEM41B (NM_015012) Human Tagged ORF Clone

CAT#: RC216351

TMEM41B (Myc-DDK-tagged)-Human transmembrane protein 41B (TMEM41B), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414863) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216351 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TMEM41B protein (Cat# TP316351). The protein was produced from HEK293T cells transfected with TMEM41B cDNA clone (Cat# RC216351) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM41B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216351 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAAGGCAGAGTCGCCGAACGATCGCAGTTGGGCGCTCACCACACGACCCCCGTGGGGGACGGGG
  CAGCGGGGACGCGGGGTCTCGCGGCGCCTGGCAGCAGAGACCACCAGAAGGAAAAATCCTGGGTAGAAGC
  TGGATCAGCAAGAATGTCACTCCTTATATTGGTGTCCATTTTCTTATCTGCAGCTTTTGTTATGTTTTTG
  GTATATAAAAATTTTCCTCAGCTTAGTGAAGAAGAAAGAGTGAATATGAAGGTTCCCAGAGATATGGATG
  ATGCCAAGGCTCTAGGAAAAGTTTTATCCAAATACAAGGACACCTTTTATGTTCAAGTACTTGTAGCTTA
  TTTTGCTACATATATTTTCTTGCAAACATTTGCTATTCCAGGCTCTATATTTCTCAGTATACTCTCAGGG
  TTTCTTTATCCCTTTCCACTAGCCTTATTTCTTGTTTGTTTGTGTTCTGGACTTGGTGCCTCTTTCTGTT
  ATATGCTTTCCTATTTAGTTGGGAGACCAGTTGTATACAAATACCTAACAGAGAAAGCAGTAAAATGGTC
  ACAGCAGGTTGAACGTCATAGAGAACATCTCATTAACTACATTATATTTTTGAGAATAACACCATTTCTG
  CCTAATTGGTTTATTAATATCACATCTCCTGTGATAAACGTGCCATTGAAAGTTTTTTTTATTGGTACTT
  TTCTAGGTGTCGCACCTCCTTCTTTTGTAGCCATTAAGGCAGGAACAACACTGTATCAACTTACAACAGC
  AGGAGAAGCTGTTTCCTGGAACTCAATATTTATTCTGATGATCTTGGCTGTTCTTTCTATTCTGCCAGCC
  ATCTTCCAAAAAAAACTAAAGCAGAAATTTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216351 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKGRVAERSQLGAHHTTPVGDGAAGTRGLAAPGSRDHQKEKSWVEAGSARMSLLILVSIFLSAAFVMFL
  VYKNFPQLSEEERVNMKVPRDMDDAKALGKVLSKYKDTFYVQVLVAYFATYIFLQTFAIPGSIFLSILSG
  FLYPFPLALFLVCLCSGLGASFCYMLSYLVGRPVVYKYLTEKAVKWSQQVERHREHLINYIIFLRITPFL
  PNWFINITSPVINVPLKVFFIGTFLGVAPPSFVAIKAGTTLYQLTTAGEAVSWNSIFILMILAVLSILPA
  IFQKKLKQKFE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015012
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015012.4
• RefSeq Size: 3973 bp
• RefSeq ORF: 876 bp
• Locus ID: 440026
• UniProt ID: Q5BJD5
• Cytogenetics: 11p15.4
• Protein Families: Transmembrane
• MW: 32.5 kDa
• Gene Summary: Required for normal motor neuron development (By similarity). Required for autophagosome formation (PubMed:30093494).[UniProtKB/Swiss-Prot Function]