SIX3 (NM_005413) Human Tagged ORF Clone

CAT#: RC216286

SIX3 (Myc-DDK-tagged)-Human SIX homeobox 3 (SIX3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SIX3 (Cat# RC216286, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX3 (Cat# TA811410)(1:2000). Positive lysates LY401662 (100ug) and LC401662 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401662) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216286 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIX3
• Synonyms: HPE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216286 representing NM_005413
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTATTCCGCTCCCCCCTAGACCTCTATTCCTCCCACTTCTTGTTGCCAAACTTCGCCGATTCTCACC
  ACCGCTCCATACTTCTGGCGAGTAGCGGCGGCGGGAACGGTGCGGGAGGCGGCGGCGGCGCGGGAGGCGG
  CAGCGGCGGCGGGAACGGTGCGGGAGGCGGCGGTGCTGGCGGAGCAGGCGGCGGCGGCGGCGGCGGCTCC
  AGGGCCCCCCCGGAAGAGTTGTCCATGTTCCAGCTGCCCACCCTCAACTTCTCGCCGGAGCAGGTGGCCA
  GCGTCTGTGAGACGCTGGAGGAGACGGGCGACATCGAGCGGCTGGGCCGCTTCCTCTGGTCGCTGCCCGT
  GGCCCCCGGGGCGTGCGAGGCCATCAACAAACACGAGTCGATCCTGCGCGCGCGCGCCGTGGTCGCCTTC
  CACACGGGCAACTTCCGCGACCTCTACCACATCCTTGAGAACCACAAGTTCACCAAGGAGTCTCACGGCA
  AGCTGCAGGCCATGTGGCTCGAGGCGCACTACCAGGAGGCCGAGAAGCTGCGCGGCCGCCCACTCGGCCC
  GGTGGACAAGTACCGCGTGCGCAAGAAGTTCCCGCTGCCACGCACCATCTGGGACGGCGAGCAGAAGACG
  CATTGCTTCAAGGAGCGGACTCGGAGCCTGTTGCGGGAGTGGTACCTACAGGACCCCTACCCCAACCCCA
  GCAAGAAACGCGAACTGGCGCAGGCCACCGGCCTCACTCCCACACAAGTAGGCAACTGGTTTAAGAACCG
  GCGGCAGCGCGACCGCGCCGCGGCGGCCAAGAACAGGCTCCAGCACCAGGCCATTGGACCGAGCGGCATG
  CGCTCGCTGGCCGAGCCCGGCTGCCCCACGCACGGCTCGGCAGAGTCGCCGTCCACGGCGGCCAGCCCGA
  CCACCAGCGTGTCCAGCCTGACGGAGCGCGCGGACACCGGCACCTCCATCCTCTCGGTAACCTCCAGCGA
  CTCGGAATGTGATGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216286 representing NM_005413
  Red=Cloning site Green=Tags(s)
  
  MVFRSPLDLYSSHFLLPNFADSHHRSILLASSGGGNGAGGGGGAGGGSGGGNGAGGGGAGGAGGGGGGGS
  RAPPEELSMFQLPTLNFSPEQVASVCETLEETGDIERLGRFLWSLPVAPGACEAINKHESILRARAVVAF
  HTGNFRDLYHILENHKFTKESHGKLQAMWLEAHYQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKT
  HCFKERTRSLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQHQAIGPSGM
  RSLAEPGCPTHGSAESPSTAASPTTSVSSLTERADTGTSILSVTSSDSECDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005413
• ORF Size: 996 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005413.1, NP_005404.1
• RefSeq Size: 1926 bp
• RefSeq ORF: 999 bp
• Locus ID: 6496
• UniProt ID: O95343
• Cytogenetics: 2p21
• Protein Families: Druggable Genome
• MW: 35.3 kDa
• Gene Summary: This gene encodes a member of the sine oculis homeobox transcription factor family. The encoded protein plays a role in eye development. Mutations in this gene have been associated with holoprosencephaly type 2. [provided by RefSeq, Oct 2009]