BRN3A (POU4F1) (NM_006237) Human Tagged ORF Clone

CAT#: RC216284

POU4F1 (Myc-DDK-tagged)-Human POU class 4 homeobox 1 (POU4F1)

Product Images

Circular map for RC216284

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  Western blot validation of overexpression lysate (Cat# LY416781) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216284 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified POU4F1 protein (Cat# TP316284). The protein was produced from HEK293T cells transfected with POU4F1 cDNA clone (Cat# RC216284) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRN3A
• Synonyms: ATITHS; brn-3A; BRN3A; Oct-T1; RDC-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216284 representing NM_006237
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCCATGAACAGCAAGCAGCCTCACTTTGCCATGCATCCCACCCTCCCTGAGCACAAGTACCCGT
  CGCTGCACTCCAGCTCCGAGGCCATCCGGCGGGCCTGCCTGCCCACGCCGCCGCTGCAGAGCAACCTCTT
  CGCCAGCCTGGACGAGACGCTGCTGGCGCGGGCCGAGGCGCTGGCGGCCGTGGACATCGCCGTGTCCCAG
  GGCAAGAGCCATCCTTTCAAGCCGGACGCCACGTACCACACGATGAACAGCGTGCCGTGCACGTCCACTT
  CCACGGTGCCTCTGGCGCACCACCACCACCACCACCACCACCACCAGGCGCTCGAACCCGGCGATCTGCT
  GGACCACATCTCCTCGCCGTCGCTCGCGCTCATGGCCGGCGCGGGCGGCGCGGGCGCGGCGGCCGGCGGC
  GGCGGCGCCCACGACGGCCCGGGGGGCGGTGGCGGCCCGGGCGGCGGCGGCGGCCCGGGCGGCGGCCCCG
  GGGGAGGCGGCGGTGGCGGCCCGGGGGGCGGCGGCGGCGGCCCGGGCGGCGGGCTCCTGGGCGGCTCCGC
  GCACCCTCACCCGCATATGCACAGCCTGGGCCACCTGTCGCACCCCGCGGCGGCGGCCGCCATGAACATG
  CCGTCCGGGCTGCCGCACCCCGGGCTGGTGGCGGCGGCGGCGCACCACGGCGCGGCAGCGGCAGCGGCGG
  CGGCGGCGGCCGGGCAGGTGGCAGCGGCATCGGCGGCGGCGGCCGTGGTGGGCGCAGCGGGCCTGGCGTC
  CATCTGCGACTCGGACACGGACCCGCGCGAGCTCGAGGCGTTCGCGGAGCGCTTCAAGCAGCGGCGCATC
  AAGCTGGGCGTGACGCAGGCCGACGTGGGCTCGGCGCTGGCCAACCTCAAGATCCCGGGCGTGGGCTCAC
  TCAGCCAGAGCACCATCTGCAGGTTCGAGTCGCTCACGCTCTCGCACAACAACATGATCGCGCTCAAGCC
  CATCCTGCAGGCGTGGCTCGAGGAGGCCGAGGGCGCCCAGCGCGAGAAAATGAACAAGCCTGAGCTCTTC
  AACGGCGGCGAGAAGAAGCGCAAGCGGACTTCCATCGCCGCGCCCGAGAAGCGCTCCCTCGAGGCCTACT
  TCGCCGTGCAGCCCCGGCCCTCGTCCGAGAAGATCGCCGCCATCGCCGAGAAACTGGACCTCAAAAAGAA
  CGTGGTGCGGGTGTGGTTTTGCAACCAGAGACAGAAGCAGAAGCGGATGAAATTCTCTGCCACTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216284 representing NM_006237
  Red=Cloning site Green=Tags(s)
  
  MMSMNSKQPHFAMHPTLPEHKYPSLHSSSEAIRRACLPTPPLQSNLFASLDETLLARAEALAAVDIAVSQ
  GKSHPFKPDATYHTMNSVPCTSTSTVPLAHHHHHHHHHQALEPGDLLDHISSPSLALMAGAGGAGAAAGG
  GGAHDGPGGGGGPGGGGGPGGGPGGGGGGGPGGGGGGPGGGLLGGSAHPHPHMHSLGHLSHPAAAAAMNM
  PSGLPHPGLVAAAAHHGAAAAAAAAAAGQVAAASAAAAVVGAAGLASICDSDTDPRELEAFAERFKQRRI
  KLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMNKPELF
  NGGEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSATY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006237
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006237.4
• RefSeq Size: 3824 bp
• RefSeq ORF: 1260 bp
• Locus ID: 5457
• UniProt ID: Q01851
• Cytogenetics: 13q31.1
• Protein Families: Transcription Factors
• MW: 42.5 kDa
• Gene Summary: This gene encodes a member of the POU-IV class of neural transcription factors. This protein is expressed in a subset of retinal ganglion cells and may be involved in the developing sensory nervous system. This protein may also promote the growth of cervical tumors. A translocation of this gene is associated with some adult acute myeloid leukemias. [provided by RefSeq, Mar 2012]