CYP26A1 (NM_000783) Human Tagged ORF Clone

CAT#: RC216278

CYP26A1 (Myc-DDK-tagged)-Human cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), transcript variant 1

Product images

Western blot validation of overexpression lysate (Cat# LY400267) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216278 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CYP26A1 protein (Cat# TP316278). The protein was produced from HEK293T cells transfected with CYP26A1 cDNA clone (Cat# RC216278) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Product Name: CYP26A1 (NM_000783) Human Tagged ORF Clone
• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP26A1
• Synonyms: CP26; CYP26; P450RAI; P450RAI1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216278 representing NM_000783
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTCCCGGCGCTGCTGGCCAGTGCGCTCTGCACCTTCGTGCTGCCGCTGCTGCTCTTCCTGGCTG
  CGATCAAGCTCTGGGACCTGTACTGCGTGAGCGGCCGCGACCGCAGTTGTGCCCTCCCATTGCCCCCCGG
  GACTATGGGCTTCCCCTTCTTTGGGGAAACCTTGCAGATGGTACTGCAGCGGAGGAAGTTCCTGCAGATG
  AAGCGCAGGAAATACGGCTTCATCTACAAGACGCATCTGTTCGGGCGGCCCACCGTACGGGTGATGGGCG
  CGGACAATGTGCGGCGCATCTTGCTCGGAGAGCACCGGCTGGTGTCGGTCCACTGGCCAGCGTCGGTGCG
  CACCATTCTGGGATCTGGCTGCCTCTCTAACCTGCACGACTCCTCGCACAAGCAGCGCAAGAAGGTGATT
  ATGCGGGCCTTCAGCCGCGAGGCACTCGAATGCTACGTGCCGGTGATCACCGAGGAAGTGGGCAGCAGCC
  TGGAGCAGTGGCTGAGCTGCGGCGAGCGCGGCCTCCTGGTCTACCCCGAGGTGAAGCGCCTCATGTTCCG
  AATCGCCATGCGCATCCTACTGGGCTGCGAACCCCAACTGGCGGGCGACGGGGACTCCGAGCAGCAGCTT
  GTGGAGGCCTTCGAGGAAATGACCCGCAATCTCTTCTCGCTGCCCATCGACGTGCCCTTCAGCGGGCTGT
  ACCGGGGCATGAAGGCGCGGAACCTCATTCACGCGCGCATCGAGCAGAACATTCGCGCCAAGATCTGCGG
  GCTGCGGGCATCCGAGGCGGGCCAGGGCTGCAAAGACGCGCTGCAGCTGTTGATCGAGCACTCGTGGGAG
  AGGGGAGAGCGGCTGGACATGCAGGCACTAAAGCAATCTTCAACCGAACTCCTCTTTGGAGGACACGAAA
  CCACGGCCAGTGCAGCCACATCTCTGATCACTTACCTGGGGCTCTACCCACATGTTCTCCAGAAAGTGCG
  AGAAGAGCTGAAGAGTAAGGGTTTACTTTGCAAGAGCAATCAAGACAACAAGTTGGACATGGAAATTTTG
  GAACAACTTAAATACATCGGGTGTGTTATTAAGGAGACCCTTCGACTGAATCCCCCAGTTCCAGGAGGGT
  TTCGGGTTGCTCTGAAGACTTTTGAATTAAATGGATACCAGATTCCCAAGGGCTGGAATGTTATCTACAG
  TATCTGTGATACTCATGATGTGGCAGAGATCTTCACCAACAAGGAAGAATTTAATCCTGACCGATTCATG
  CTGCCTCACCCAGAGGATGCATCCAGGTTCAGCTTCATTCCATTTGGAGGAGGCCTTAGGAGCTGTGTAG
  GCAAAGAATTTGCAAAAATTCTTCTCAAAATATTTACAGTGGAGCTGGCCAGGCATTGTGACTGGCAGCT
  TCTAAATGGACCTCCTACAATGAAAACCAGTCCCACCGTGTATCCTGTGGACAATCTCCCTGCAAGATTC
  ACCCATTTCCATGGGGAAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216278 representing NM_000783
  Red=Cloning site Green=Tags(s)
  
  MGLPALLASALCTFVLPLLLFLAAIKLWDLYCVSGRDRSCALPLPPGTMGFPFFGETLQMVLQRRKFLQM
  KRRKYGFIYKTHLFGRPTVRVMGADNVRRILLGEHRLVSVHWPASVRTILGSGCLSNLHDSSHKQRKKVI
  MRAFSREALECYVPVITEEVGSSLEQWLSCGERGLLVYPEVKRLMFRIAMRILLGCEPQLAGDGDSEQQL
  VEAFEEMTRNLFSLPIDVPFSGLYRGMKARNLIHARIEQNIRAKICGLRASEAGQGCKDALQLLIEHSWE
  RGERLDMQALKQSSTELLFGGHETTASAATSLITYLGLYPHVLQKVREELKSKGLLCKSNQDNKLDMEIL
  EQLKYIGCVIKETLRLNPPVPGGFRVALKTFELNGYQIPKGWNVIYSICDTHDVAEIFTNKEEFNPDRFM
  LPHPEDASRFSFIPFGGGLRSCVGKEFAKILLKIFTVELARHCDWQLLNGPPTMKTSPTVYPVDNLPARF
  THFHGEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• Tag: Myc-DDK
• ACCN: NM_000783
• ORF Size: 1491 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000783.1, NM_000783.2, NM_000783.3, NP_000774.2
• RefSeq Size: 2099 bp
• RefSeq ORF: 1494 bp
• Locus ID: 1592
• Cytogenetics: 10q23.33
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Retinol metabolism
• MW: 56 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]