IGF2BP1 (NM_006546) Human Tagged ORF Clone

CAT#: RC216226

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IGF2BP1 (Myc-DDK-tagged)-Human insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1), transcript variant 1



  "NM_006546" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 537.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol IGF2BP1
Synonyms CRD-BP; CRDBP; IMP-1; IMP1; VICKZ1; ZBP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC216226 representing NM_006546
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAGCTTTACATCGGCAACCTCAACGAGAGCGTGACCCCCGCGGACTTGGAGAAAGTGTTTGCGG
AGCACAAGATCTCCTACAGCGGCCAGTTCTTGGTCAAATCCGGCTACGCCTTCGTGGACTGCCCGGACGA
GCACTGGGCGATGAAGGCCATCGAAACTTTCTCCGGGAAAGTAGAATTACAAGGAAAACGCTTAGAGATT
GAACATTCGGTGCCCAAAAAACAAAGGAGCCGGAAAATTCAAATCCGAAATATTCCACCCCAGCTCCGAT
GGGAAGTACTGGACAGCCTGCTGGCTCAGTATGGTACAGTAGAGAACTGTGAGCAAGTGAACACCGAGAG
TGAGACGGCAGTGGTGAATGTCACCTATTCCAACCGGGAGCAGACCAGGCAAGCCATCATGAAGCTGAAT
GGCCACCAGTTGGAGAACCATGCCCTGAAGGTCTCCTACATCCCCGATGAGCAGATAGCACAGGGACCTG
AGAATGGGCGCCGAGGGGGCTTTGGCTCTCGGGGTCAGCCCCGCCAGGGCTCACCTGTGGCAGCGGGGGC
CCCAGCCAAGCAGCAGCAAGTGGACATCCCCCTTCGGCTCCTGGTGCCCACCCAGTATGTGGGTGCCATT
ATTGGCAAGGAGGGGGCCACCATCCGCAACATCACAAAACAGACCCAGTCCAAGATAGACGTGCATAGGA
AGGAGAACGCAGGTGCAGCTGAAAAAGCCATCAGTGTGCACTCCACCCCTGAGGGCTGCTCCTCCGCTTG
TAAGATGATCTTGGAGATTATGCATAAAGAGGCTAAGGACACCAAAACGGCTGACGAGGTTCCCCTGAAG
ATCCTGGCCCATAATAACTTTGTAGGGCGTCTCATTGGCAAGGAAGGACGGAACCTGAAGAAGGTAGAGC
AAGATACCGAGACAAAAATCACCATCTCCTCGTTGCAAGACCTTACCCTTTACAACCCTGAGAGGACCAT
CACTGTGAAGGGGGCCATCGAGAATTGTTGCAGGGCCGAGCAGGAAATAATGAAGAAAGTTCGGGAGGCC
TATGAGAATGATGTGGCTGCCATGAGCCTGCAGTCTCACCTGATCCCTGGCCTGAACCTGGCTGCTGTAG
GTCTTTTCCCAGCTTCATCCAGCGCAGTCCCGCCGCCTCCCAGCAGCGTTACTGGGGCTGCTCCCTATAG
CTCCTTTATGCAGGCTCCCGAGCAGGAGATGGTGCAGGTGTTTATCCCCGCCCAGGCAGTGGGCGCCATC
ATCGGCAAGAAGGGGCAGCACATCAAACAGCTCTCCCGGTTTGCCAGCGCCTCCATCAAGATTGCACCAC
CCGAAACACCTGACTCCAAAGTTCGTATGGTTATCATCACTGGACCGCCAGAGGCCCAATTCAAGGCTCA
GGGAAGAATCTATGGCAAACTCAAGGAGGAGAACTTCTTTGGTCCCAAGGAGGAAGTGAAGCTGGAGACC
CACATACGTGTGCCAGCATCAGCAGCTGGCCGGGTCATTGGCAAAGGTGGAAAAACGGTGAACGAGTTGC
AGAATTTGACGGCAGCTGAGGTGGTAGTACCAAGAGACCAGACCCCTGATGAGAACGACCAGGTCATCGT
GAAAATCATCGGACATTTCTATGCCAGTCAGATGGCTCAACGGAAGATCCGAGACATCCTGGCCCAGGTT
AAGCAGCAGCATCAGAAGGGACAGAGTAACCAGGCCCAGGCACGGAGGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC216226 representing NM_006546
Red=Cloning site Green=Tags(s)

MNKLYIGNLNESVTPADLEKVFAEHKISYSGQFLVKSGYAFVDCPDEHWAMKAIETFSGKVELQGKRLEI
EHSVPKKQRSRKIQIRNIPPQLRWEVLDSLLAQYGTVENCEQVNTESETAVVNVTYSNREQTRQAIMKLN
GHQLENHALKVSYIPDEQIAQGPENGRRGGFGSRGQPRQGSPVAAGAPAKQQQVDIPLRLLVPTQYVGAI
IGKEGATIRNITKQTQSKIDVHRKENAGAAEKAISVHSTPEGCSSACKMILEIMHKEAKDTKTADEVPLK
ILAHNNFVGRLIGKEGRNLKKVEQDTETKITISSLQDLTLYNPERTITVKGAIENCCRAEQEIMKKVREA
YENDVAAMSLQSHLIPGLNLAAVGLFPASSSAVPPPPSSVTGAAPYSSFMQAPEQEMVQVFIPAQAVGAI
IGKKGQHIKQLSRFASASIKIAPPETPDSKVRMVIITGPPEAQFKAQGRIYGKLKEENFFGPKEEVKLET
HIRVPASAAGRVIGKGGKTVNELQNLTAAEVVVPRDQTPDENDQVIVKIIGHFYASQMAQRKIRDILAQV
KQQHQKGQSNQAQARRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006546
ORF Size 1731 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006546.4
RefSeq Size 8769 bp
RefSeq ORF 1734 bp
Locus ID 10642
UniProt ID Q9NZI8
Cytogenetics 17q21.32
Domains RRM, KH
MW 63.3 kDa
Gene Summary This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

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