IGF2BP1 (NM_006546) Human Tagged ORF Clone

CAT#: RC216226

IGF2BP1 (Myc-DDK-tagged)-Human insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1), transcript variant 1

Product Images

Circular map for RC216226

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  Western blot validation of overexpression lysate (Cat# LY401963) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216226 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IGF2BP1 protein (Cat# TP316226). The protein was produced from HEK293T cells transfected with IGF2BP1 cDNA clone (Cat# RC216226) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IGF2BP1
• Synonyms: CRD-BP; CRDBP; IMP-1; IMP1; VICKZ1; ZBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216226 representing NM_006546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAGCTTTACATCGGCAACCTCAACGAGAGCGTGACCCCCGCGGACTTGGAGAAAGTGTTTGCGG
  AGCACAAGATCTCCTACAGCGGCCAGTTCTTGGTCAAATCCGGCTACGCCTTCGTGGACTGCCCGGACGA
  GCACTGGGCGATGAAGGCCATCGAAACTTTCTCCGGGAAAGTAGAATTACAAGGAAAACGCTTAGAGATT
  GAACATTCGGTGCCCAAAAAACAAAGGAGCCGGAAAATTCAAATCCGAAATATTCCACCCCAGCTCCGAT
  GGGAAGTACTGGACAGCCTGCTGGCTCAGTATGGTACAGTAGAGAACTGTGAGCAAGTGAACACCGAGAG
  TGAGACGGCAGTGGTGAATGTCACCTATTCCAACCGGGAGCAGACCAGGCAAGCCATCATGAAGCTGAAT
  GGCCACCAGTTGGAGAACCATGCCCTGAAGGTCTCCTACATCCCCGATGAGCAGATAGCACAGGGACCTG
  AGAATGGGCGCCGAGGGGGCTTTGGCTCTCGGGGTCAGCCCCGCCAGGGCTCACCTGTGGCAGCGGGGGC
  CCCAGCCAAGCAGCAGCAAGTGGACATCCCCCTTCGGCTCCTGGTGCCCACCCAGTATGTGGGTGCCATT
  ATTGGCAAGGAGGGGGCCACCATCCGCAACATCACAAAACAGACCCAGTCCAAGATAGACGTGCATAGGA
  AGGAGAACGCAGGTGCAGCTGAAAAAGCCATCAGTGTGCACTCCACCCCTGAGGGCTGCTCCTCCGCTTG
  TAAGATGATCTTGGAGATTATGCATAAAGAGGCTAAGGACACCAAAACGGCTGACGAGGTTCCCCTGAAG
  ATCCTGGCCCATAATAACTTTGTAGGGCGTCTCATTGGCAAGGAAGGACGGAACCTGAAGAAGGTAGAGC
  AAGATACCGAGACAAAAATCACCATCTCCTCGTTGCAAGACCTTACCCTTTACAACCCTGAGAGGACCAT
  CACTGTGAAGGGGGCCATCGAGAATTGTTGCAGGGCCGAGCAGGAAATAATGAAGAAAGTTCGGGAGGCC
  TATGAGAATGATGTGGCTGCCATGAGCCTGCAGTCTCACCTGATCCCTGGCCTGAACCTGGCTGCTGTAG
  GTCTTTTCCCAGCTTCATCCAGCGCAGTCCCGCCGCCTCCCAGCAGCGTTACTGGGGCTGCTCCCTATAG
  CTCCTTTATGCAGGCTCCCGAGCAGGAGATGGTGCAGGTGTTTATCCCCGCCCAGGCAGTGGGCGCCATC
  ATCGGCAAGAAGGGGCAGCACATCAAACAGCTCTCCCGGTTTGCCAGCGCCTCCATCAAGATTGCACCAC
  CCGAAACACCTGACTCCAAAGTTCGTATGGTTATCATCACTGGACCGCCAGAGGCCCAATTCAAGGCTCA
  GGGAAGAATCTATGGCAAACTCAAGGAGGAGAACTTCTTTGGTCCCAAGGAGGAAGTGAAGCTGGAGACC
  CACATACGTGTGCCAGCATCAGCAGCTGGCCGGGTCATTGGCAAAGGTGGAAAAACGGTGAACGAGTTGC
  AGAATTTGACGGCAGCTGAGGTGGTAGTACCAAGAGACCAGACCCCTGATGAGAACGACCAGGTCATCGT
  GAAAATCATCGGACATTTCTATGCCAGTCAGATGGCTCAACGGAAGATCCGAGACATCCTGGCCCAGGTT
  AAGCAGCAGCATCAGAAGGGACAGAGTAACCAGGCCCAGGCACGGAGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216226 representing NM_006546
  Red=Cloning site Green=Tags(s)
  
  MNKLYIGNLNESVTPADLEKVFAEHKISYSGQFLVKSGYAFVDCPDEHWAMKAIETFSGKVELQGKRLEI
  EHSVPKKQRSRKIQIRNIPPQLRWEVLDSLLAQYGTVENCEQVNTESETAVVNVTYSNREQTRQAIMKLN
  GHQLENHALKVSYIPDEQIAQGPENGRRGGFGSRGQPRQGSPVAAGAPAKQQQVDIPLRLLVPTQYVGAI
  IGKEGATIRNITKQTQSKIDVHRKENAGAAEKAISVHSTPEGCSSACKMILEIMHKEAKDTKTADEVPLK
  ILAHNNFVGRLIGKEGRNLKKVEQDTETKITISSLQDLTLYNPERTITVKGAIENCCRAEQEIMKKVREA
  YENDVAAMSLQSHLIPGLNLAAVGLFPASSSAVPPPPSSVTGAAPYSSFMQAPEQEMVQVFIPAQAVGAI
  IGKKGQHIKQLSRFASASIKIAPPETPDSKVRMVIITGPPEAQFKAQGRIYGKLKEENFFGPKEEVKLET
  HIRVPASAAGRVIGKGGKTVNELQNLTAAEVVVPRDQTPDENDQVIVKIIGHFYASQMAQRKIRDILAQV
  KQQHQKGQSNQAQARRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006546
• ORF Size: 1731 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_006546.4
• RefSeq Size: 8769 bp
• RefSeq ORF: 1734 bp
• Locus ID: 10642
• UniProt ID: Q9NZI8
• Cytogenetics: 17q21.32
• Domains: RRM, KH
• MW: 63.3 kDa
• Gene Summary: This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]