VAV2 (NM_003371) Human Tagged ORF Clone

CAT#: RC216175

VAV2 (Myc-DDK-tagged)-Human vav 2 guanine nucleotide exchange factor (VAV2), transcript variant 2

Product Images

Circular map for RC216175

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  Western blot validation of overexpression lysate (Cat# LY418726) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216175 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified VAV2 protein (Cat# TP316175). The protein was produced from HEK293T cells transfected with VAV2 cDNA clone (Cat# RC216175) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VAV2
• Synonyms: VAV-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216175 representing NM_003371
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGTGGCGACAGTGCGGCCGCTGGCTCATCGATTGCAAGGTCCTGCCGCCCAACCACCGGGTGG
  TGTGGCCCTCGGCCGTGGTCTTCGACCTGGCGCAGGCGCTGCGCGACGGGGTCCTTCTGTGCCAGCTGCT
  GCACAACCTCTCCCCCGGCTCCATCGACCTCAAGGACATCAACTTCCGGCCGCAGATGTCCCAGTTTCTG
  TGTTTGAAGAACATACGCACCTTCCTGAAAGTCTGCCACGATAAATTTGGATTAAGGAACAGCGAGCTGT
  TTGACCCCTTTGACCTCTTCGATGTGCGAGACTTTGGAAAGGTCATCTCCGCGGTGTCGAGGCTCTCCCT
  GCACAGCATCGCGCAGAACAAAGGGATCAGGCCTTTTCCCTCAGAGGAGACCACAGAGAATGACGATGAC
  GTCTACCGCAGCCTGGAGGAGCTGGCCGACGAGCATGACCTGGGGGAGGACATCTACGACTGCGTCCCGT
  GTGAGGATGGAGGGGACGACATCTACGAGGACATCATCAAGGTGGAGGTGCAGCAGCCCATGAAAATGGG
  CATGACTGAAGATGACAAGAGGAACTGCTGCCTGCTGGAGATCCAGGAGACCGAGGCCAAGTACTACCGC
  ACCCTGGAGGACATTGAGAAGAACTACATGAGCCCCCTGCGGCTGGTGCTGAGCCCGGCGGACATGGCAG
  CTGTCTTCATTAACCTGGAGGACCTGATCAAGGTGCATCACAGCTTCCTGAGGGCCATCGACGTGTCCGT
  GATGGTGGGGGGCAGCACGCTGGCCAAGGTCTTCCTCGATTTCAAGGAAAGGCTTCTGATCTACGGGGAG
  TACTGCAGCCACATGGAGCACGCCCAGAACACACTGAACCAGCTCCTGGCCAGCCGGGAGGACTTCAGGC
  AGAAAGTCGAGGAGTGCACACTGAAGGTCCAGGATGGAAAATTTAAGCTGCAAGACCTGCTGGTGGTCCC
  CATGCAGAGGGTGCTCAAATACCACCTGCTCTTGAAGGAGCTTCTGAGCCATTCTGCGGAACGGCCTGAG
  AGGCAGCAGCTCAAAGAAGCACTGGAAGCCATGCAGGACTTGGCGATGTACATCAATGAAGTTAAACGGG
  ACAAGGAGACCTTGAGGAAAATCAGCGAATTTCAGAGTTCTATAGAAAATTTGCAAGTGAAACTGGAGGA
  ATTTGGAAGACCAAAGATTGACGGGGAACTGAAAGTCCGGTCCATAGTCAACCACACCAAGCAGGACAGG
  TACTTGTTCCTGTTTGACAAGGTGGTCATCGTCTGCAAGCGGAAGGGCTACAGCTACGAGCTCAAGGAGA
  TCATCGAGCTGCTGTTCCACAAGATGACCGACGACCCCATGAACAACAAGGACGTCAAGAAGTGGTCCTA
  CGGCTTCTACCTAATTCACCTTCAAGGAAAGCAGGGCTTCCAGTTTTTCTGCAAAACAGAAGATATGAAG
  AGGAAGTGGATGGAGCAGTTTGAGATGGCCATGTCAAACATCAAGCCAGACAAAGCCAATGCCAACCACC
  ACAGTTTCCAGATGTACACGTTTGACAAGACCACCAACTGCAAAGCCTGCAAAATGTTCCTCAGGGGCAC
  CTTCTACCAGGGATACATGTGTACCAAGTGTGGCGTCGGGGCACACAAGGAGTGCCTGGAAGTGATACCT
  CCCTGCAAGTTCACTTCTCCTGCAGATCTGGACGCCTCCGGAGCGGGACCAGGTCCCAAGATGGTGGCCA
  TGCAGAATTACCATGGCAACCCAGCCCCTCCCGGGAAGCCTGTGCTGACCTTCCAGACGGGCGACGTGCT
  TGAGCTGCTGAGGGGCGACCCTGAGTCTCCGTGGTGGGAGGGTCGTCTGGTACAAACCAGGAAGTCAGGG
  TATTTCCCCAGCTCATCTGTGAAGCCCTGCCCTGTGGATGGAAGGCCGCCCATCAGCCGGCCGCCATCCC
  GGGAGATCGACTACACTGCATACCCCTGGTTTGCAGGTAACATGGAGAGGCAGCAGACGGACAACCTGCT
  CAAGTCCCACGCCAGCGGGACCTACCTGATCAGGGAGCGGCCTGCCGAGGCTGAGCGCTTTGCAATAAGC
  ATCAAGTTCAATGATGAGGTGAAGCACATCAAGGTGGTGGAGAAGGACAACTGGATCCACATCACAGAGG
  CCAAGAAATTCGACAGCCTCCTGGAGTTGGTGGAGTACTACCAGTGCCACTCACTGAAGGAGAGCTTCAA
  GCAGCTGGACACCACACTCAAGTACCCCTACAAGTCCCGGGAACGTTCGGCCTCCAGGGCCTCCAGCCGG
  TCCCCAGTGTTCACGCCCCGCGTCATCGGCACAGCTGTGGCCAGGTATAACTTTGCCGCCCGAGATATGA
  GGGAGCTTTCGCTGCGGGAGGGTGACGTGGTGAGGATCTACAGCCGCATCGGCGGAGACCAGGGCTGGTG
  GAAGGGCGAGACCAACGGACGGATTGGCTGGTTTCCTTCAACGTACGTAGAAGAGGAGGGCATCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216175 representing NM_003371
  Red=Cloning site Green=Tags(s)
  
  MEQWRQCGRWLIDCKVLPPNHRVVWPSAVVFDLAQALRDGVLLCQLLHNLSPGSIDLKDINFRPQMSQFL
  CLKNIRTFLKVCHDKFGLRNSELFDPFDLFDVRDFGKVISAVSRLSLHSIAQNKGIRPFPSEETTENDDD
  VYRSLEELADEHDLGEDIYDCVPCEDGGDDIYEDIIKVEVQQPMKMGMTEDDKRNCCLLEIQETEAKYYR
  TLEDIEKNYMSPLRLVLSPADMAAVFINLEDLIKVHHSFLRAIDVSVMVGGSTLAKVFLDFKERLLIYGE
  YCSHMEHAQNTLNQLLASREDFRQKVEECTLKVQDGKFKLQDLLVVPMQRVLKYHLLLKELLSHSAERPE
  RQQLKEALEAMQDLAMYINEVKRDKETLRKISEFQSSIENLQVKLEEFGRPKIDGELKVRSIVNHTKQDR
  YLFLFDKVVIVCKRKGYSYELKEIIELLFHKMTDDPMNNKDVKKWSYGFYLIHLQGKQGFQFFCKTEDMK
  RKWMEQFEMAMSNIKPDKANANHHSFQMYTFDKTTNCKACKMFLRGTFYQGYMCTKCGVGAHKECLEVIP
  PCKFTSPADLDASGAGPGPKMVAMQNYHGNPAPPGKPVLTFQTGDVLELLRGDPESPWWEGRLVQTRKSG
  YFPSSSVKPCPVDGRPPISRPPSREIDYTAYPWFAGNMERQQTDNLLKSHASGTYLIRERPAEAERFAIS
  IKFNDEVKHIKVVEKDNWIHITEAKKFDSLLELVEYYQCHSLKESFKQLDTTLKYPYKSRERSASRASSR
  SPVFTPRVIGTAVARYNFAARDMRELSLREGDVVRIYSRIGGDQGWWKGETNGRIGWFPSTYVEEEGIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003371
• ORF Size: 2517 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003371.2, NP_003362.2
• RefSeq Size: 4707 bp
• RefSeq ORF: 2520 bp
• Locus ID: 7410
• UniProt ID: P52735
• Cytogenetics: 9q34.2
• Domains: RhoGEF, SH2, SH3, CH, PH, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, T cell receptor signaling pathway
• MW: 96.9 kDa
• Gene Summary: VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]