HIPK1 (NM_198268) Human Tagged ORF Clone

CAT#: RC216108

HIPK1 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 1 (HIPK1), transcript variant 1

Product Images

Circular map for RC216108

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HIPK1
• Synonyms: Myak; Nbak2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216108 representing NM_198268
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCACAGCTGCAAGTGTTTTCGCCCCCATCAGTGTCGTCGAGTGCCTTCTGCAGTGCGAAGAAAC
  TGAAAATAGAGCCCTCTGGCTGGGATGTTTCAGGACAGAGTAGCAACGACAAATATTATACCCACAGCAA
  AACCCTCCCAGCCACACAAGGGCAAGCCAACTCCTCTCACCAGGTAGCAAATTTCAACATCCCTGCTTAC
  GACCAGGGCCTCCTCCTCCCAGCTCCTGCAGTGGAGCATATTGTTGTAACAGCCGCTGATAGCTCGGGCA
  GTGCTGCTACATCAACCTTCCAAAGCAGCCAGACCCTGACTCACAGAAGCAACGTTTCTTTGCTTGAGCC
  ATATCAAAAATGTGGATTGAAACGAAAAAGTGAGGAAGTTGACAGCAACGGTAGTGTGCAGATCATAGAA
  GAACATCCCCCTCTCATGCTGCAAAACAGGACTGTGGTGGGTGCTGCTGCCACAACCACCACTGTGACCA
  CAAAGAGTAGCAGTTCCAGCGGAGAAGGGGATTACCAGCTGGTCCAGCATGAGATCCTTTGCTCTATGAC
  CAATAGCTATGAAGTCTTGGAGTTCCTAGGCCGGGGGACATTTGGACAGGTGGCTAAGTGCTGGAAGAGG
  AGCACCAAGGAAATTGTGGCTATTAAAATCTTGAAGAACCACCCCTCCTATGCCAGACAAGGACAGATTG
  AAGTGAGCATCCTTTCCCGCCTAAGCAGTGAAAATGCTGATGAGTATAATTTTGTCCGTTCATACGAGTG
  CTTTCAGCATAAGAATCACACCTGCCTTGTTTTTGAAATGTTGGAGCAGAACTTATATGATTTTCTAAAG
  CAAAACAAATTTAGCCCACTGCCACTCAAGTACATCAGACCAATCTTGCAGCAGGTGGCCACAGCCTTGA
  TGAAGCTCAAGAGTCTTGGTCTGATCCACGCTGACCTTAAGCCTGAAAACATCATGCTGGTTGATCCAGT
  TCGCCAGCCCTACCGAGTGAAGGTCATTGACTTTGGTTCTGCTAGTCACGTTTCCAAAGCTGTGTGCTCA
  ACCTACTTACAGTCACGTTACTACAGAGCTCCTGAAATTATTCTTGGGTTACCATTTTGTGAAGCTATTG
  ATATGTGGTCACTGGGCTGTGTGATAGCTGAGCTGTTCCTGGGATGGCCTCTTTATCCTGGTGCTTCAGA
  ATATGATCAGATTCGTTATATTTCACAAACACAAGGCTTGCCAGCTGAATATCTTCTCAGTGCCGGAACA
  AAAACAACCAGGTTTTTCAACAGAGATCCTAATTTGGGGTACCCACTGTGGAGGCTTAAGACACCTGAAG
  AACATGAACTGGAGACTGGAATAAAATCAAAAGAAGCTCGGAAGTACATTTTTAATTGCTTAGATGACAT
  GGCTCAGGTGAATATGTCTACAGACCTGGAGGGAACAGACATGTTGGCAGAGAAGGCAGACCGAAGAGAA
  TACATTGATCTGTTAAAGAAAATGCTCACAATTGATGCAGATAAGAGAATTACCCCTCTAAAAACTCTTA
  ACCATCAGTTTGTGACAATGACTCACCTTTTGGATTTTCCACATAGCAATCATGTTAAGTCTTGTTTTCA
  GAACATGGAGATCTGCAAGCGGAGGGTTCACATGTATGATACAGTGAGTCAGATCAAGAGTCCCTTCACT
  ACACATGTTGCCCCAAATACAAGCACAAATCTAACCATGAGCTTCAGCAATCAGCTCAATACAGTGCACA
  ATCAGGCCAGTGTTCTAGCTTCCAGTTCTACTGCAGCAGCTGCTACTCTTTCTCTGGCTAATTCAGATGT
  CTCACTACTAAACTACCAGTCAGCTTTGTACCCATCATCTGCTGCACCAGTTCCTGGAGTTGCCCAGCAG
  GGTGTTTCCTTGCAGCCTGGAACCACCCAGATTTGCACTCAGACAGATCCATTCCAACAGACATTTATAG
  TATGTCCACCTGCGTTTCAAACTGGACTACAAGCAACAACAAAGCATTCTGGATTCCCTGTGAGGATGGA
  TAATGCTGTACCGATTGTACCCCAGGCACCAGCTGCTCAGCCACTACAGATTCAGTCAGGAGTTCTCACG
  CAGGGAAGCTGTACACCACTAATGGTAGCAACTCTCCACCCTCAAGTAGCCACCATCACACCGCAGTATG
  CGGTGCCCTTTACTCTGAGCTGCGCAGCCGGCCGGCCGGCGCTGGTTGAACAGACTGCCGCTGTACTGCA
  GGCGTGGCCTGGAGGGACTCAGCAAATTCTCCTGCCTTCAACTTGGCAACAGTTGCCTGGGGTAGCTCTA
  CACAACTCTGTCCAGCCCACAGCAATGATTCCAGAGGCCATGGGGAGTGGACAGCAGCTAGCTGACTGGA
  GGAATGCCCACTCTCATGGCAACCAGTACAGCACTATCATGCAGCAGCCATCCTTGCTGACTAACCATGT
  GACATTGGCCACTGCTCAGCCTCTGAATGTTGGTGTTGCCCATGTTGTCAGACAACAACAATCCAGTTCC
  CTCCCTTCGAAGAAGAATAAGCAGTCAGCTCCAGTCTCTTCCAAGTCCTCTCTAGATGTTCTGCCTTCCC
  AAGTCTATTCTCTGGTTGGGAGCAGTCCCCTCCGCACCACATCTTCTTATAATTCCTTGGTCCCTGTCCA
  AGATCAGCATCAGCCCATCATCATTCCAGATACTCCCAGCCCTCCTGTGAGTGTCATCACTATCCGAAGT
  GACACTGATGAGGAAGAGGACAACAAATACAAGCCCAGTAGCTCTGGACTGAAGCCAAGGTCTAATGTCA
  TCAGTTATGTCACTGTCAATGATTCTCCAGACTCTGACTCTTCTTTGAGCAGCCCTTATTCCACTGATAC
  CCTGAGTGCTCTCCGAGGCAATAGTGGATCCGTTTTGGAGGGGCCTGGCAGAGTTGTGGCAGATGGCACT
  GGCACCCGCACTATCATTGTGCCTCCACTGAAAACTCAGCTTGGTGACTGCACTGTAGCAACCCAGGCCT
  CAGGTCTCCTGAGCAATAAGACTAAGCCAGTCGCTTCAGTGAGTGGGCAGTCATCTGGATGCTGTATCAC
  CCCCACAGGGTATCGAGCTCAACGCGGGGGGACCAGTGCAGCACAACCACTCAATCTTAGCCAGAACCAG
  CAGTCATCGGCGGCTCCAACCTCACAGGAGAGAAGCAGCAACCCAGCCCCCCGCAGGCAGCAGGCGTTTG
  TGGCCCCTCTCTCCCAAGCCCCCTACACCTTCCAGCATGGCAGCCCGCTACACTCGACAGGGCACCCACA
  CCTTGCCCCGGCCCCTGCTCACCTGCCAAGCCAGGCTCATCTGTATACGTATGCTGCCCCGACTTCTGCT
  GCTGCACTGGGCTCAACCAGCTCCATTGCTCATCTTTTCTCCCCACAGGGTTCCTCAAGGCATGCTGCAG
  CCTATACCACTCACCCTAGCACTTTGGTGCACCAGGTCCCTGTCAGTGTTGGGCCCAGCCTCCTCACTTC
  TGCCAGCGTGGCCCCTGCTCAGTACCAACACCAGTTTGCCACCCAATCCTACATTGGGTCTTCCCGAGGC
  TCAACAATTTACACTGGATACCCGCTGAGTCCTACCAAGATCAGCCAGTATTCCTACTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216108 representing NM_198268
  Red=Cloning site Green=Tags(s)
  
  MASQLQVFSPPSVSSSAFCSAKKLKIEPSGWDVSGQSSNDKYYTHSKTLPATQGQANSSHQVANFNIPAY
  DQGLLLPAPAVEHIVVTAADSSGSAATSTFQSSQTLTHRSNVSLLEPYQKCGLKRKSEEVDSNGSVQIIE
  EHPPLMLQNRTVVGAAATTTTVTTKSSSSSGEGDYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKR
  STKEIVAIKILKNHPSYARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLK
  QNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCS
  TYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGT
  KTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMSTDLEGTDMLAEKADRRE
  YIDLLKKMLTIDADKRITPLKTLNHQFVTMTHLLDFPHSNHVKSCFQNMEICKRRVHMYDTVSQIKSPFT
  THVAPNTSTNLTMSFSNQLNTVHNQASVLASSSTAAAATLSLANSDVSLLNYQSALYPSSAAPVPGVAQQ
  GVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKHSGFPVRMDNAVPIVPQAPAAQPLQIQSGVLT
  QGSCTPLMVATLHPQVATITPQYAVPFTLSCAAGRPALVEQTAAVLQAWPGGTQQILLPSTWQQLPGVAL
  HNSVQPTAMIPEAMGSGQQLADWRNAHSHGNQYSTIMQQPSLLTNHVTLATAQPLNVGVAHVVRQQQSSS
  LPSKKNKQSAPVSSKSSLDVLPSQVYSLVGSSPLRTTSSYNSLVPVQDQHQPIIIPDTPSPPVSVITIRS
  DTDEEEDNKYKPSSSGLKPRSNVISYVTVNDSPDSDSSLSSPYSTDTLSALRGNSGSVLEGPGRVVADGT
  GTRTIIVPPLKTQLGDCTVATQASGLLSNKTKPVASVSGQSSGCCITPTGYRAQRGGTSAAQPLNLSQNQ
  QSSAAPTSQERSSNPAPRRQQAFVAPLSQAPYTFQHGSPLHSTGHPHLAPAPAHLPSQAHLYTYAAPTSA
  AALGSTSSIAHLFSPQGSSRHAAAYTTHPSTLVHQVPVSVGPSLLTSASVAPAQYQHQFATQSYIGSSRG
  STIYTGYPLSPTKISQYSYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198268
• ORF Size: 3630 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198268.3
• RefSeq Size: 8155 bp
• RefSeq ORF: 3633 bp
• Locus ID: 204851
• UniProt ID: Q86Z02
• Cytogenetics: 1p13.2
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• MW: 130.8 kDa
• Gene Summary: The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]