POU4F2 (NM_004575) Human Tagged ORF Clone

CAT#: RC215962

POU4F2 (Myc-DDK-tagged)-Human POU class 4 homeobox 2 (POU4F2)

Product Images

Circular map for RC215962

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  Western blot validation of overexpression lysate (Cat# LY417894) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215962 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified POU4F2 protein (Cat# TP315962). The protein was produced from HEK293T cells transfected with POU4F2 cDNA clone (Cat# RC215962) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POU4F2
• Synonyms: Brn-3b; BRN3.2; BRN3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215962 representing NM_004575
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATGATGTCCCTGAACAGCAAGCAGGCGTTTAGCATGCCGCACGGCGGCAGCCTGCACGTGGAGC
  CCAAGTACTCGGCACTGCACAGCACCTCGCCGGGCTCCTCGGCTCCCATCGCGCCCTCGGCCAGCTCCCC
  CAGCAGCTCGAGCAACGCTGGTGGTGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGAGGCCGA
  AGCAGCAGCTCCAGCAGCAGTGGCAGCAGCGGCGGCGGGGGCTCGGAGGCTATGCGGAGAGCCTGTCTTC
  CAACCCCACCGAGCAATATATTCGGCGGGCTGGATGAGAGTCTGCTGGCCCGCGCCGAGGCTCTGGCAGC
  CGTGGACATCGTCTCCCAGAGCAAGAGCCACCACCACCATCCACCCCACCACAGCCCCTTCAAACCGGAC
  GCCACCTACCACACTATGAATACCATCCCGTGCACGTCGGCCGCCTCTTCTTCATCGGTGCCCATCTCGC
  ACCCTTGCGCGTTGGCGGGCACGCACCACCACCACCACCATCACCACCACCACCACCACCAACCGCACCA
  GGCGCTGGAGGGCGAGCTGCTGGAGCACCTGAGTCCCGGGCTGGCCCTGGGCGCTATGGCGGGCCCCGAC
  GGCGCTGTGGTGTCCACGCCGGCTCACGCGCCGCACATGGCCACCATGAACCCCATGCACCAAGCAGCGC
  TCAGCATGGCCCACGCGCACGGGCTGCCGTCGCACATGGGCTGCATGAGCGACGTGGACGCCGACCCGCG
  GGACCTGGAGGCATTCGCCGAGCGCTTCAAGCAGCGACGCATCAAGCTGGGGGTGACCCAGGCAGATGTG
  GGCTCCGCGCTGGCCAACCTCAAGATCCCCGGCGTGGGCTCGCTTAGCCAGAGCACCATCTGCAGGTTCG
  AGTCCCTCACACTGTCCCACAATAATATGATCGCGCTCAAACCCATCCTGCAGGCATGGCTCGAGGAGGC
  CGAGAAGTCCCACCGCGAGAAGCTCACCAAGCCTGAACTCTTCAATGGCGCGGAGAAGAAGCGCAAGCGC
  ACGTCCATCGCTGCGCCAGAGAAGCGCTCGCTCGAAGCCTACTTTGCCATTCAGCCTCGGCCCTCCTCTG
  AAAAGATCGCCGCCATCGCGGAGAAGCTGGACCTGAAGAAAAACGTGGTGCGCGTCTGGTTCTGCAACCA
  GAGGCAGAAACAGAAAAGAATGAAATATTCCGCCGGCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215962 representing NM_004575
  Red=Cloning site Green=Tags(s)
  
  MMMMSLNSKQAFSMPHGGSLHVEPKYSALHSTSPGSSAPIAPSASSPSSSSNAGGGGGGGGGGGGGGGGR
  SSSSSSSGSSGGGGSEAMRRACLPTPPSNIFGGLDESLLARAEALAAVDIVSQSKSHHHHPPHHSPFKPD
  ATYHTMNTIPCTSAASSSSVPISHPCALAGTHHHHHHHHHHHHQPHQALEGELLEHLSPGLALGAMAGPD
  GAVVSTPAHAPHMATMNPMHQAALSMAHAHGLPSHMGCMSDVDADPRDLEAFAERFKQRRIKLGVTQADV
  GSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGAEKKRKR
  TSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004575
• ORF Size: 1230 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004575.1, NP_004566.1
• RefSeq Size: 3110 bp
• RefSeq ORF: 1230 bp
• Locus ID: 5458
• UniProt ID: Q12837
• Cytogenetics: 4q31.22
• Protein Families: Transcription Factors
• MW: 42.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the POU-domain transcription factor family and may be involved in maintaining visual system neurons in the retina. The level of the encoded protein is also elevated in a majority of breast cancers, resulting in accelerated tumor growth. [provided by RefSeq, Sep 2011]