CARD9 (NM_052813) Human Tagged ORF Clone

CAT#: RC215899

CARD9 (Myc-DDK-tagged)-Human caspase recruitment domain family, member 9 (CARD9), transcript variant 1

Product Images

Circular map for RC215899

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CARD9 (Cat# RC215899, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CARD9(Cat# TA811909). Positive lysates LY409473 (100ug) and LC409473 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY409473) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215899 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CARD9 protein (Cat# TP315899). The protein was produced from HEK293T cells transfected with CARD9 cDNA clone (Cat# RC215899) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CARD9
• Synonyms: CANDF2; hCARD9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215899 representing NM_052813
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGACTACGAGAACGATGACGAGTGCTGGAACGTCCTGGAGGGCTTCCGGGTGACGCTCACCTCGG
  TCATCGACCCCTCACGCATCACACCTTACCTGCGGCAGTGCAAGGTCCTGAACCCTGATGATGAGGAGCA
  GGTGCTCAGCGACCCCAACCTGGTCATCCGCAAACGGAAAGTGGGTGTGCTCCTGGACATCCTGCAGCGG
  ACCGGCCACAAGGGCTACGTGGCCTTCCTCGAGAGCCTGGAGCTCTACTACCCGCAGCTGTACAAGAAGG
  TCACAGGCAAGGAGCCGGCCCGCGTCTTCTCCATGATCATCGACGCGTCCGGGGAGTCAGGCCTGACTCA
  GCTGCTGATGACTGAGGTCATGAAGCTGCAGAAGAAGGTGCAGGACCTGACCGCGCTGCTGAGCTCCAAA
  GATGACTTCATCAAGGAGCTGCGGGTGAAGGACAGCCTGCTGCGCAAGCACCAGGAGCGTGTGCAGAGGC
  TCAAGGAGGAGTGCGAGGCCGGCAGCCGCGAGCTCAAGCGCTGCAAGGAGGAGAACTACGACCTGGCCAT
  GCGCCTGGCGCACCAGAGTGAGGAGAAGGGCGCCGCGCTCATGCGGAACCGTGACCTGCAGCTGGAGATT
  GACCAGCTCAAGCACAGCCTCATGAAGGCCGAGGACGACTGCAAGGTGGAGCGCAAGCACACGCTGAAGC
  TCAGGCACGCCATGGAGCAGCGGCCCAGCCAGGAGCTGCTGTGGGAGCTGCAGCAGGAGAAGGCCCTGCT
  CCAGGCCCGGGTGCAGGAGCTGGAGGCCTCCGTCCAGGAGGGGAAGCTGGACAGGAGCAGCCCCTACATC
  CAGGTACTGGAGGAGGACTGGCGGCAGGCGCTGCGGGACCACCAGGAGCAGGCCAACACCATCTTCTCCC
  TGCGCAAGGACCTCCGCCAGGGCGAGGCCCGACGCCTCCGGTGCATGGAGGAGAAGGAGATGTTCGAGCT
  GCAGTGCCTGGCACTACGTAAGGACTCCAAGATGTACAAGGACCGCATCGAGGCCATCCTGCTGCAGATG
  GAGGAGGTCGCCATTGAGCGGGACCAGGCCATAGCCACGCGGGAGGAGCTGCACGCACAGCACGCCCGGG
  GCCTGCAGGAGAAGGACGCGCTGCGCAAGCAGGTGCGGGAGCTGGGCGAGAAGGCGGATGAGCTGCAGCT
  GCAGGTGTTCCAGTGTGAGGCGCAGCTACTGGCCGTGGAGGGCAGGCTCAGGCGGCAGCAGCTGGAGACG
  CTCGTCCTGAGCTCCGACCTGGAAGATGGCTCACCCAGGAGGTCCCAGGAGCTCTCACTCCCCCAGGACC
  TGGAGGACACCCAGCTCTCAGACAAAGGCTGCCTTGCCGGCGGGGGGAGCCCGAAACAGCCCTTTGCAGC
  TCTGCACCAGGAGCAGGTTTTGCGGAACCCCCATGACGCAGGCCTGAGCAGCGGGGAGCCGCCCGAGAAG
  GAGCGGCGGCGCCTCAAAGAGAGTTTTGAGAACTACCGCAGGAAGCGCGCCCTCAGGAAGATGCAGAAAG
  GATGGCGGCAGGGGGAGGAGGACCGGGAGAACACCACGGGCAGCGACAACACCGACACTGAGGGCTCC
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215899 representing NM_052813
  Red=Cloning site Green=Tags(s)
  
  MSDYENDDECWNVLEGFRVTLTSVIDPSRITPYLRQCKVLNPDDEEQVLSDPNLVIRKRKVGVLLDILQR
  TGHKGYVAFLESLELYYPQLYKKVTGKEPARVFSMIIDASGESGLTQLLMTEVMKLQKKVQDLTALLSSK
  DDFIKELRVKDSLLRKHQERVQRLKEECEAGSRELKRCKEENYDLAMRLAHQSEEKGAALMRNRDLQLEI
  DQLKHSLMKAEDDCKVERKHTLKLRHAMEQRPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYI
  QVLEEDWRQALRDHQEQANTIFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQM
  EEVAIERDQAIATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLET
  LVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDAGLSSGEPPEK
  ERRRLKESFENYRRKRALRKMQKGWRQGEEDRENTTGSDNTDTEGS
  
  TRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052813
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052813.5
• RefSeq Size: 2136 bp
• RefSeq ORF: 1611 bp
• Locus ID: 64170
• UniProt ID: Q9H257
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 62.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq, Jul 2008]