TRPS1 (NM_014112) Human Tagged ORF Clone

CAT#: RC215856

TRPS1 (Myc-DDK-tagged)-Human trichorhinophalangeal syndrome I (TRPS1)

Product Images

Circular map for RC215856

• 

  Western blot validation of overexpression lysate (Cat# LY415485) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215856 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRPS1 (Cat# RC215856, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPS1 (Cat# TA813179)(1:500).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPS1
• Synonyms: GC79; LGCR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215856 representing NM_014112
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTATGAAGTCAATGCTGGGTATGATTTTACAAATATGGTCCGGAAAAAGAACCCCCCTCTGAGAA
  ACGTTGCAAGTGAAGGCGAGGGCCAGATCCTGGAGCCTATAGGTACAGAAAGCAAGGTATCTGGAAAGAA
  CAAAGAATTTTCTGCAGATCAGATGTCAGAAAATACGGATCAGAGTGATGCTGCAGAACTAAATCATAAG
  GAGGAACATAGCTTGCATGTTCAAGATCCATCTTCTAGCAGTAAGAAGGACTTGAAAAGCGCAGTTCTGA
  GTGAGAAGGCTGGCTTCAATTATGAAAGCCCCAGTAAGGGAGGAAACTTTCCCTCCTTTCCGCATGATGA
  GGTGACAGACAGAAATATGTTGGCTTTCTCATCTCCAGCTGCTGGGGGAGTCTGTGAGCCCTTGAAGTCT
  CCGCAAAGAGCAGAGGCAGATGACCCTCAAGATATGGCCTGCACCCCCTCAGGGGACTCACTGGAGACAA
  AGGAAGATCAGAAGATGTCACCAAAGGCTACAGAGGAAACAGGGCAAGCACAGAGTGGTCAAGCCAATTG
  TCAAGGTTTGAGCCCAGTTTCAGTGGCCTCAAAAAACCCACAAGTGCCTTCAGATGGGGGTGTAAGACTG
  AATAAATCCAAAACTGACTTACTGGTGAATGACAACCCAGACCCGGCACCTCTGTCTCCAGAGCTTCAGG
  ACTTTAAATGCAATATCTGTGGATATGGTTACTACGGCAACGACCCCACAGATCTGATTAAGCACTTCCG
  AAAGTATCACTTAGGACTGCATAACCGCACCAGGCAAGATGCTGAGCTGGACAGCAAAATCTTGGCCCTT
  CATAACATGGTGCAGTTCAGCCATTCCAAAGACTTCCAGAAGGTCAACCGTTCTGTGTTTTCTGGTGTGC
  TGCAGGACATCAATTCTTCAAGGCCTGTTTTACTAAATGGGACCTATGATGTGCAGGTGACTTCAGGTGG
  AACATTCATTGGCATTGGACGGAAAACACCAGATTGCCAAGGGAACACCAAGTATTTCCGCTGTAAATTC
  TGCAATTTCACTTATATGGGCAACTCATCCACCGAATTAGAACAACATTTTCTTCAGACTCACCCAAACA
  AAATAAAAGCTTCTCTCCCCTCCTCTGAGGTTGCAAAACCTTCAGAGAAAAACTCTAACAAGTCCATCCC
  TGCACTTCAATCCAGTGATTCTGGAGACTTGGGAAAATGGCAGGACAAGATAACAGTCAAAGCAGGAGAT
  GACACTCCTGTTGGGTACTCAGTGCCCATAAAGCCCCTCGATTCCTCTAGACAAAATGGTACAGAGGCCA
  CCAGTTACTACTGGTGTAAATTTTGTAGTTTCAGCTGTGAGTCATCTAGCTCACTTAAACTGCTAGAACA
  TTATGGCAAGCAGCACGGAGCAGTGCAGTCAGGCGGCCTTAATCCAGAGTTAAATGATAAGCTTTCCAGG
  GGCTCTGTCATTAATCAGAATGATCTAGCCAAAAGTTCAGAAGGAGAGACAATGACCAAGACAGACAAGA
  GCTCGAGTGGGGCTAAAAAGAAGGACTTCTCCAGCAAGGGAGCCGAGGATAATATGGTAACGAGCTATAA
  TTGTCAGTTCTGTGACTTCCGATATTCCAAAAGCCATGGCCCTGATGTAATTGTAGTGGGGCCACTTCTC
  CGTCATTATCAACAGCTCCATAACATTCACAAGTGTACCATTAAACACTGTCCATTCTGTCCCAGAGGAC
  TTTGCAGCCCAGAAAAGCACCTTGGAGAAATTACTTATCCGTTTGCTTGTAGAAAAAGTAATTGTTCCCA
  CTGTGCACTCTTGCTTCTGCACTTGTCTCCTGGGGCGGCTGGAAGCTCGCGAGTCAAACATCAGTGCCAT
  CAGTGTTCATTCACCACCCCTGACGTAGATGTACTCCTCTTTCACTATGAAAGTGTGCATGAGTCCCAAG
  CATCGGATGTCAAACAAGAAGCAAATCACCTGCAAGGATCGGATGGGCAGCAGTCTGTCAAGGAAAGCAA
  AGAACACTCATGTACCAAATGTGATTTTATTACCCAAGTGGAAGAAGAGATTTCCCGACACTACAGGAGA
  GCACACAGCTGCTACAAATGCCGTCAGTGCAGTTTTACAGCTGCCGATACTCAGTCACTACTGGAGCACT
  TCAACACTGTTCACTGCCAGGAACAGGACATCACTACAGCCAACGGCGAAGAGGACGGTCATGCCATATC
  CACCATCAAAGAGGAGCCCAAAATTGACTTCAGGGTCTACAATCTGCTAACTCCAGACTCTAAAATGGGA
  GAGCCAGTTTCTGAGAGTGTGGTGAAGAGAGAGAAGCTGGAAGAGAAGGACGGGCTCAAAGAGAAAGTTT
  GGACCGAGAGTTCCAGTGATGACCTTCGCAATGTGACTTGGAGAGGGGCAGACATCCTGCGGGGGAGTCC
  GTCATACACCCAAGCAAGCCTGGGGCTGCTGACGCCTGTGTCTGGCACCCAAGAGCAGACAAAGACTCTA
  AGGGATAGTCCCAATGTGGAGGCCGCCCATCTGGCGCGACCTATTTATGGCTTGGCTGTGGAAACCAAGG
  GATTCCTGCAGGGGGCGCCAGCTGGCGGAGAGAAGTCTGGGGCCCTCCCCCAGCAGTATCCTGCATCGGG
  AGAAAACAAGTCCAAGGATGAATCCCAGTCCCTGTTACGGAGGCGTAGAGGCTCCGGTGTTTTTTGTGCC
  AATTGCCTGACCACAAAGACCTCTCTCTGGCGAAAGAATGCAAATGGCGGATATGTATGCAACGCGTGTG
  GCCTCTACCAGAAGCTTCACTCGACTCCCAGGCCTTTAAACATCATTAAACAAAACAACGGTGAGCAGAT
  TATTAGGAGGAGAACAAGAAAGCGCCTTAACCCAGAGGCACTTCAGGCTGAGCAGCTCAACAAACAGCAG
  AGGGGCAGCAATGAGGAGCAAGTCAATGGAAGCCCGTTAGAGAGGAGGTCAGAAGATCATCTAACTGAAA
  GTCACCAGAGAGAAATTCCACTCCCCAGCCTAAGTAAATACGAAGCCCAGGGTTCATTGACTAAAAGCCA
  TTCTGCTCAGCAGCCAGTCCTGGTCAGCCAAACTCTGGATATTCACAAAAGGATGCAACCTTTGCACATT
  CAGATAAAAAGTCCTCAGGAAAGTACTGGAGATCCAGGAAATAGTTCATCCGTATCTGAAGGGAAAGGAA
  GTTCTGAGAGAGGCAGTCCTATAGAAAAGTACATGAGACCTGCGAAACACCCAAATTATTCACCACCAGG
  CAGCCCTATTGAAAAGTACCAGTACCCACTTTTTGGACTTCCCTTTGTACATAATGACTTCCAGAGTGAA
  GCTGATTGGCTGCGGTTCTGGAGTAAATATAAGCTCTCCGTTCCTGGGAATCCGCACTACTTGAGTCACG
  TGCCTGGCCTACCAAATCCTTGCCAAAACTATGTGCCTTATCCCACCTTCAATCTGCCTCCTCATTTTTC
  AGCTGTTGGATCAGACAATGACATTCCTCTAGATTTGGCGATCAAGCATTCCAGACCTGGGCCAACTGCA
  AACGGTGCCTCCAAGGAGAAAACGAAGGCACCACCAAATGTAAAAAATGAAGGTCCCTTGAATGTAGTAA
  AAACAGAGAAAGTTGATAGAAGTACTCAAGATGAACTTTCAACAAAATGTGTGCACTGTGGCATTGTCTT
  TCTGGATGAAGTGATGTATGCTTTGCATATGAGTTGCCATGGTGACAGTGGACCTTTCCAGTGCAGCATA
  TGCCAGCATCTTTGCACGGACAAATATGACTTCACAACACATATCCAGAGGGGCCTGCATAGGAACAATG
  CACAAGTGGAAAAAAATGGAAAACCTAAAGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215856 representing NM_014112
  Red=Cloning site Green=Tags(s)
  
  MPYEVNAGYDFTNMVRKKNPPLRNVASEGEGQILEPIGTESKVSGKNKEFSADQMSENTDQSDAAELNHK
  EEHSLHVQDPSSSSKKDLKSAVLSEKAGFNYESPSKGGNFPSFPHDEVTDRNMLAFSSPAAGGVCEPLKS
  PQRAEADDPQDMACTPSGDSLETKEDQKMSPKATEETGQAQSGQANCQGLSPVSVASKNPQVPSDGGVRL
  NKSKTDLLVNDNPDPAPLSPELQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILAL
  HNMVQFSHSKDFQKVNRSVFSGVLQDINSSRPVLLNGTYDVQVTSGGTFIGIGRKTPDCQGNTKYFRCKF
  CNFTYMGNSSTELEQHFLQTHPNKIKASLPSSEVAKPSEKNSNKSIPALQSSDSGDLGKWQDKITVKAGD
  DTPVGYSVPIKPLDSSRQNGTEATSYYWCKFCSFSCESSSSLKLLEHYGKQHGAVQSGGLNPELNDKLSR
  GSVINQNDLAKSSEGETMTKTDKSSSGAKKKDFSSKGAEDNMVTSYNCQFCDFRYSKSHGPDVIVVGPLL
  RHYQQLHNIHKCTIKHCPFCPRGLCSPEKHLGEITYPFACRKSNCSHCALLLLHLSPGAAGSSRVKHQCH
  QCSFTTPDVDVLLFHYESVHESQASDVKQEANHLQGSDGQQSVKESKEHSCTKCDFITQVEEEISRHYRR
  AHSCYKCRQCSFTAADTQSLLEHFNTVHCQEQDITTANGEEDGHAISTIKEEPKIDFRVYNLLTPDSKMG
  EPVSESVVKREKLEEKDGLKEKVWTESSSDDLRNVTWRGADILRGSPSYTQASLGLLTPVSGTQEQTKTL
  RDSPNVEAAHLARPIYGLAVETKGFLQGAPAGGEKSGALPQQYPASGENKSKDESQSLLRRRRGSGVFCA
  NCLTTKTSLWRKNANGGYVCNACGLYQKLHSTPRPLNIIKQNNGEQIIRRRTRKRLNPEALQAEQLNKQQ
  RGSNEEQVNGSPLERRSEDHLTESHQREIPLPSLSKYEAQGSLTKSHSAQQPVLVSQTLDIHKRMQPLHI
  QIKSPQESTGDPGNSSSVSEGKGSSERGSPIEKYMRPAKHPNYSPPGSPIEKYQYPLFGLPFVHNDFQSE
  ADWLRFWSKYKLSVPGNPHYLSHVPGLPNPCQNYVPYPTFNLPPHFSAVGSDNDIPLDLAIKHSRPGPTA
  NGASKEKTKAPPNVKNEGPLNVVKTEKVDRSTQDELSTKCVHCGIVFLDEVMYALHMSCHGDSGPFQCSI
  CQHLCTDKYDFTTHIQRGLHRNNAQVEKNGKPKE
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014112
• ORF Size: 3882 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014112.5
• RefSeq Size: 9990 bp
• RefSeq ORF: 3885 bp
• Locus ID: 7227
• UniProt ID: Q9UHF7
• Cytogenetics: 8q23.3
• Domains: GATA, zf-C2H2
• Protein Families: Druggable Genome, Transcription Factors
• MW: 142.8 kDa
• Gene Summary: This gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III. [provided by RefSeq, Jul 2008]