OGFR (NM_007346) Human Tagged ORF Clone

CAT#: RC215844

OGFR (Myc-DDK-tagged)-Human opioid growth factor receptor (OGFR). Note: ORF is codon optimized

Product Images

Circular map for RC215844

• 

  Coomassie blue staining of purified OGFR protein (Cat# TP315844). The protein was produced from HEK293T cells transfected with OGFR cDNA clone (Cat# RC215844) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OGFR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215844 ORF sequence, codon optimized.
  Due to the complexity of NM_007346, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGATCCCGACTGCGACTCCACTTGGGAAGAGGATGAGGAGGACGCAGAGGATGCAGAAGATGAGG
  ATTGTGAAGACGGAGAGGCAGCCGGCGCCAGGGACGCCGACGCGGGTGACGAGGATGAGGAGTCCGAGGA
  ACCGAGAGCAGCAAGACCATCTAGCTTTCAATCCCGAATGACCGGAAGTAGAAACTGGCGGGCGACAAGA
  GACATGTGTAGATACCGGCATAACTACCCTGATTTGGTGGAAAGAGACTGCAATGGGGACACACCTAATC
  TCAGCTTTTATAGGAACGAAATTCGCTTTCTGCCTAATGGGTGCTTTATCGAAGATATACTCCAAAACTG
  GACCGACAACTACGACCTGCTCGAAGATAACCACTCTTATATCCAGTGGCTGTTCCCGCTTAGGGAACCC
  GGCGTGAATTGGCATGCCAAACCTCTGACCCTGCGCGAGGTGGAAGTCTTTAAAAGTTCACAGGAGATCC
  AGGAGAGACTCGTTAGAGCCTATGAGCTTATGCTCGGATTTTATGGGATTAGGCTGGAGGATCGCGGTAC
  CGGCACTGTCGGACGCGCCCAGAACTATCAGAAGCGGTTTCAAAACCTGAACTGGAGAAGCCACAACAAT
  CTCCGCATCACTCGGATCCTCAAGAGCCTGGGCGAACTCGGCCTCGAACACTTTCAGGCTCCGTTGGTTC
  GCTTCTTCCTCGAGGAAACTCTCGTGCGCAGAGAACTGCCCGGAGTTCGGCAGTCCGCACTGGATTACTT
  CATGTTCGCAGTGCGCTGCAGACACCAGAGGAGACAACTGGTACACTTTGCCTGGGAACATTTCAGACCC
  AGGTGTAAATTTGTATGGGGCCCACAGGATAAACTGCGACGGTTTAAACCATCAAGTCTGCCTCACCCAC
  TGGAGGGGAGCAGAAAGGTAGAAGAAGAGGGATCACCTGGAGATCCTGACCACGAAGCATCTACCCAAGG
  AAGGACCTGCGGACCTGAACACAGCAAAGGAGGAGGCAGGGTCGACGAAGGTCCTCAGCCTCGGTCCGTG
  GAGCCACAGGATGCAGGACCATTGGAGAGGAGCCAGGGAGACGAGGCCGGAGGTCACGGCGAGGACCGGC
  CCGAACCTCTGTCACCTAAGGAATCTAAAAAACGGAAACTTGAGCTCTCCAGGAGAGAGCAGCCCCCCAC
  AGAGCCAGGACCACAGAGCGCTTCCGAAGTGGAGAAGATTGCATTGAACCTGGAGGGCTGCGCACTGAGC
  CAAGGTTCATTGCGGACCGGTACGCAGGAAGTCGGAGGCCAGGACCCTGGAGAAGCAGTGCAGCCATGTC
  GGCAGCCTCTTGGCGCCAGGGTAGCCGATAAGGTGAGAAAGAGACGCAAGGTGGACGAAGGTGCCGGGGA
  TTCAGCTGCTGTGGCTTCAGGAGGCGCCCAGACACTTGCGCTGGCCGGTTCACCTGCGCCGTCAGGACAC
  CCAAAGGCTGGCCATAGTGAGAATGGAGTGGAGGAAGACACCGAGGGGAGAACTGGGCCAAAGGAAGGGA
  CCCCTGGGAGTCCTAGTGAGACTCCCGGGCCAAGCCCTGCTGGACCGGCAGGCGATGAGCCCGCGGAATC
  ACCATCCGAGACACCAGGCCCACGGCCCGCTGGACCAGCGGGCGACGAACCGGCCGAAAGTCCTTCAGAA
  ACACCTGGACCAAGACCGGCCGGCCCGGCTGGAGACGAGCCAGCCGAGAGCCCTTCAGAGACACCCGGCC
  CTTCCCCCGCAGGACCCACTAGAGACGAGCCGGCAGAGTCTCCCAGCGAAACACCCGGCCCCCGCCCTGC
  TGGCCCCGCTGGGGATGAGCCAGCAGAGAGCCCGAGTGAAACACCCGGTCCGAGGCCCGCCGGCCCAGCG
  GGTGATGAACCAGCCGAAAGCCCCTCTGAAACTCCCGGTCCTAGCCCCGCTGGACCTACCAGGGACGAGC
  CAGCCAAGGCCGGAGAAGCTGCTGAACTGCAAGACGCCGAAGTAGAGTCTAGTGCGAAGAGCGGGAAACC
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215844 representing NM_007346
  Red=Cloning site Green=Tags(s)
  
  MDDPDCDSTWEEDEEDAEDAEDEDCEDGEAAGARDADAGDEDEESEEPRAARPSSFQSRMTGSRNWRATR
  DMCRYRHNYPDLVERDCNGDTPNLSFYRNEIRFLPNGCFIEDILQNWTDNYDLLEDNHSYIQWLFPLREP
  GVNWHAKPLTLREVEVFKSSQEIQERLVRAYELMLGFYGIRLEDRGTGTVGRAQNYQKRFQNLNWRSHNN
  LRITRILKSLGELGLEHFQAPLVRFFLEETLVRRELPGVRQSALDYFMFAVRCRHQRRQLVHFAWEHFRP
  RCKFVWGPQDKLRRFKPSSLPHPLEGSRKVEEEGSPGDPDHEASTQGRTCGPEHSKGGGRVDEGPQPRSV
  EPQDAGPLERSQGDEAGGHGEDRPEPLSPKESKKRKLELSRREQPPTEPGPQSASEVEKIALNLEGCALS
  QGSLRTGTQEVGGQDPGEAVQPCRQPLGARVADKVRKRRKVDEGAGDSAAVASGGAQTLALAGSPAPSGH
  PKAGHSENGVEEDTEGRTGPKEGTPGSPSETPGPSPAGPAGDEPAESPSETPGPRPAGPAGDEPAESPSE
  TPGPRPAGPAGDEPAESPSETPGPSPAGPTRDEPAESPSETPGPRPAGPAGDEPAESPSETPGPRPAGPA
  GDEPAESPSETPGPSPAGPTRDEPAKAGEAAELQDAEVESSAKSGKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007346
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007346.2, NM_007346.3, NP_031372.2
• RefSeq Size: 2410 bp
• RefSeq ORF: 2034 bp
• Locus ID: 11054
• UniProt ID: Q9NZT2
• Cytogenetics: 20q13.33
• Domains: OGFr_N, OGFr_repeat
• Protein Families: Druggable Genome
• MW: 73.3 kDa
• Gene Summary: The protein encoded by this gene is a receptor for opioid growth factor (OGF), also known as [Met(5)]-enkephalin. OGF is a negative regulator of cell proliferation and tissue organization in a variety of processes. The encoded unbound receptor for OGF has been localized to the outer nuclear envelope, where it binds OGF and is translocated into the nucleus. The coding sequence of this gene contains a polymorphic region of 60 nt tandem imperfect repeat units. Several transcripts containing between zero and eight repeat units have been reported. [provided by RefSeq, Jul 2008]