BMP2K (NM_198892) Human Tagged ORF Clone

CAT#: RC215795

BMP2K (Myc-DDK-tagged)-Human BMP2 inducible kinase (BMP2K), transcript variant 1

Product Images

Circular map for RC215795

• 

  Western blot validation of overexpression lysate (Cat# LY404770) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215795 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified BMP2K protein (Cat# TP315795). The protein was produced from HEK293T cells transfected with BMP2K cDNA clone (Cat# RC215795) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMP2K
• Synonyms: BIKE; HRIHFB2017
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215795 representing NM_198892
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGTTCTCTCGGATGCCCAAGTCGGAGGGCGGCAGCGGCGGCGGAGCGGCGGGTGGCGGGGCTG
  GCGGGGCCGGGGCCGGGGCCGGCTGCGGCTCCGGCGGCTCGTCCGTGGGGGTCCGGGTGTTCGCGGTCGG
  CCGCCACCAGGTCACCCTGGAAGAGTCGCTGGCCGAAGGTGGATTCTCCACAGTTTTCCTCGTGCGTACT
  CACGGTGGAATCCGATGTGCATTGAAGCGAATGTATGTCAATAACATGCCAGACCTCAATGTTTGTAAAA
  GGGAAATTACAATTATGAAAGAGCTATCTGGTCACAAAAATATTGTGGGCTATTTGGACTGTGCTGTTAA
  TTCAATTAGTGATAATGTATGGGAAGTCCTTATCTTAATGGAATATTGTCGAGCTGGACAGGTAGTGAAT
  CAAATGAATAAGAAGCTACAGACGGGTTTTACAGAACCAGAAGTGTTACAGATATTCTGTGATACCTGTG
  AAGCTGTTGCAAGGTTGCATCAGTGTAAGACTCCAATAATTCACCGGGATCTGAAGGTAGAAAATATTTT
  GTTGAATGATGGTGGGAACTATGTACTTTGTGACTTTGGCAGTGCCACTAATAAATTTCTTAATCCTCAA
  AAAGATGGAGTTAATGTAGTAGAAGAAGAAATTAAAAAGTATACAACTCTGTCATACAGAGCCCCTGAAA
  TGATCAACCTTTATGGAGGGAAACCCATCACCACCAAGGCTGATATCTGGGCACTGGGATGTCTACTCTA
  TAAACTTTGTTTCTTCACTCTTCCTTTTGGTGAGAGTCAGGTTGCTATCTGTGATGGCAACTTCACCATC
  CCAGACAATTCTCGTTACTCCCGTAACATACATTGCTTAATAAGGTTCATGCTTGAACCAGATCCGGAAC
  ATAGACCTGATATATTTCAAGTGTCATATTTTGCATTTAAATTTGCCAAAAAGGATTGTCCAGTCTCCAA
  CATCAATAATTCTTCTATTCCTTCAGCTCTTCCTGAACCGATGACTGCTAGTGAAGCAGCTGCTAGGAAA
  AGCCAAATAAAAGCCAGAATAACAGATACCATTGGACCAACAGAAACCTCAATTGCACCAAGACAAAGAC
  CAAAGGCCAACTCTGCTACTACTGCCACTCCCAGTGTGCTGACCATTCAAAGTTCAGCAACACCTGTTAA
  AGTCCTTGCTCCTGGTGAATTCGGTAACCATAGACCAAAAGGGGCACTAAGACCTGGAAATGGCCCTGAA
  ATTTTATTGGGTCAGGGACCTCCTCAGCAGCCGCCACAGCAGCATAGAGTACTCCAGCAACTACAGCAGG
  GAGATTGGAGATTACAGCAACTCCATTTACAGCATCGTCATCCTCACCAGCAGCAGCAGCAGCAGCAGCA
  GCAACAGCAACAGCAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCACCACCACCAC
  CACCACCACCACCTACTTCAAGATGCTTATATGCAGCAGTATCAACATGCAACACAGCAGCAACAGATGC
  TTCAACAACAATTTTTAATGCATTCGGTATATCAACCACAACCTTCTGCATCACAGTATCCTACAATGAT
  GCCGCAGTATCAGCAGGCTTTCTTTCAACAGCAGATGCTAGCTCAACATCAGCCGTCTCAACAACAGGCA
  TCACCTGAATATCTTACCTCCCCTCAAGAGTTCTCACCAGCCTTAGTTTCCTACACTTCATCACTTCCAG
  CTCAGGTTGGAACCATAATGGACTCCTCCTATAGTGCCAATAGGTCAGTTGCTGATAAAGAGGCCATTGC
  AAATTTCACAAATCAGAAGAACATCAGCAATCCACCTGATATGTCAGGGTGGAATCCTTTTGGAGAGGAT
  AATTTCTCTAAGTTAACAGAAGAGGAACTATTGGACAGAGAATTTGACCTTCTAAGATCAAATAGGCTCG
  AGGAGAGAGCATCCTCAGATAAGAATGTAGACTCACTTTCTGCTCCACATAACCATCCTCCAGAAGATCC
  TTTTGGTTCTGTTCCTTTCATTTCTCATTCAGGTTCTCCTGAAAAGAAAGCTGAACATTCATCTATAAAT
  CAAGAAAATGGCACTGCAAACCCTATCAAGAACGGTAAAACAAGTCCAGCATCTAAAGATCAGCGGACTG
  GAAAGAAAACCTCAGTACAGGGTCAAGTGCAAAAGGGGAATGATGAATCTGAAAGTGATTTTGAATCAGA
  TCCCCCTTCTCCTAAGAGCAGTGAAGAGGAAGAGCAAGATGATGAAGAAGTTCTTCAGGGGGAACAAGGA
  GATTTTAATGATGATGATACTGAACCAGAAAATCTGGGTCATAGGCCTCTCCTCATGGATTCTGAAGATG
  AGGAAGAAGAGGAGAAACATAGCTCTGATTCTGATTATGAGCAGGCTAAAGCAAAGTACAGTGACATGAG
  CTCTGTCTACAGAGACAGATCTGGCAGTGGACCAACCCAAGATCTTAATACAATACTCCTCACCTCAGCC
  CAATTATCCTCTGATGTTGCAGTGGAGACTCCCAAACAGGAGTTTGATGTATTTGGCGCTGTCCCCTTCT
  TTGCAGTGCGTGCTCAACAGCCCCAGCAAGAAAAGAATGAAAAGAACCTCCCTCAACACAGGTTTCCTGC
  TGCAGGACTGGAGCAGGAGGAATTTGATGTATTCACAAAGGCGCCTTTTAGCAAGAAGGTGAATGTACAA
  GAATGCCATGCAGTGGGGCCTGAGGCACATACTATCCCTGGTTATCCCAAAAGTGTAGATGTATTTGGCT
  CCACTCCATTTCAGCCCTTCCTCACATCAACAAGTAAAAGTGAAAGCAATGAGGACCTTTTTGGGCTTGT
  GCCCTTTGATGAAATAACGGGGAGCCAGCAGCAAAAAGTCAAACAGCGCAGCTTACAGAAACTGTCCTCT
  CGCCAAAGGCGCACAAAGCAGGATATGTCCAAAAGTAATGGGAAGCGGCATCATGGCACGCCAACTAGCA
  CAAAGAAGACTTTGAAGCCTACCTATCGCACTCCAGAGAGGGCTCGCAGGCACAAAAAAGTGGGCCGCCG
  AGACTCTCAAAGTAGCAATGAATTTTTAACCATCTCAGACTCCAAGGAGAACATTAGTGTTGCACTGACT
  GATGGGAAAGATAGGGGGAATGTCTTACAACCTGAGGAGAGCCTGTTGGACCCCTTCGGTGCCAAGCCCT
  TCCATTCTCCAGACCTGTCATGGCACCCTCCACATCAGGGCCTGAGCGACATCCGTGCTGATCACAATAC
  TGTCCTGCCAGGGCGGCCAAGACAAAATTCACTACATGGGTCATTCCATAGTGCAGATGTATTGAAAATG
  GATGATTTTGGTGCCGTGCCCTTTACAGAACTTGTGGTGCAAAGCATCACTCCACATCAGTCCCAACAGT
  CCCAACCAGTCGAATTAGACCCATTTGGTGCTGCTCCATTTCCTTCTAAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215795 representing NM_198892
  Red=Cloning site Green=Tags(s)
  
  MKKFSRMPKSEGGSGGGAAGGGAGGAGAGAGCGSGGSSVGVRVFAVGRHQVTLEESLAEGGFSTVFLVRT
  HGGIRCALKRMYVNNMPDLNVCKREITIMKELSGHKNIVGYLDCAVNSISDNVWEVLILMEYCRAGQVVN
  QMNKKLQTGFTEPEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLNDGGNYVLCDFGSATNKFLNPQ
  KDGVNVVEEEIKKYTTLSYRAPEMINLYGGKPITTKADIWALGCLLYKLCFFTLPFGESQVAICDGNFTI
  PDNSRYSRNIHCLIRFMLEPDPEHRPDIFQVSYFAFKFAKKDCPVSNINNSSIPSALPEPMTASEAAARK
  SQIKARITDTIGPTETSIAPRQRPKANSATTATPSVLTIQSSATPVKVLAPGEFGNHRPKGALRPGNGPE
  ILLGQGPPQQPPQQHRVLQQLQQGDWRLQQLHLQHRHPHQQQQQQQQQQQQQQQQQQQQQQQQQQQHHHH
  HHHHLLQDAYMQQYQHATQQQQMLQQQFLMHSVYQPQPSASQYPTMMPQYQQAFFQQQMLAQHQPSQQQA
  SPEYLTSPQEFSPALVSYTSSLPAQVGTIMDSSYSANRSVADKEAIANFTNQKNISNPPDMSGWNPFGED
  NFSKLTEEELLDREFDLLRSNRLEERASSDKNVDSLSAPHNHPPEDPFGSVPFISHSGSPEKKAEHSSIN
  QENGTANPIKNGKTSPASKDQRTGKKTSVQGQVQKGNDESESDFESDPPSPKSSEEEEQDDEEVLQGEQG
  DFNDDDTEPENLGHRPLLMDSEDEEEEEKHSSDSDYEQAKAKYSDMSSVYRDRSGSGPTQDLNTILLTSA
  QLSSDVAVETPKQEFDVFGAVPFFAVRAQQPQQEKNEKNLPQHRFPAAGLEQEEFDVFTKAPFSKKVNVQ
  ECHAVGPEAHTIPGYPKSVDVFGSTPFQPFLTSTSKSESNEDLFGLVPFDEITGSQQQKVKQRSLQKLSS
  RQRRTKQDMSKSNGKRHHGTPTSTKKTLKPTYRTPERARRHKKVGRRDSQSSNEFLTISDSKENISVALT
  DGKDRGNVLQPEESLLDPFGAKPFHSPDLSWHPPHQGLSDIRADHNTVLPGRPRQNSLHGSFHSADVLKM
  DDFGAVPFTELVVQSITPHQSQQSQPVELDPFGAAPFPSKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198892
• ORF Size: 3483 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198892.1, NP_942595.1
• RefSeq Size: 3859 bp
• RefSeq ORF: 3486 bp
• Locus ID: 55589
• UniProt ID: Q9NSY1
• Cytogenetics: 4q21.21
• Protein Families: Druggable Genome, Protein Kinase
• MW: 129 kDa
• Gene Summary: This gene is the human homolog of mouse BMP-2-inducible kinase. Bone morphogenic proteins (BMPs) play a key role in skeletal development and patterning. Expression of the mouse gene is increased during BMP-2 induced differentiation and the gene product is a putative serine/threonine protein kinase containing a nuclear localization signal. Therefore, the protein encoded by this human homolog is thought to be a protein kinase with a putative regulatory role in attenuating the program of osteoblast differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]