SLC6A20 (NM_020208) Human Tagged ORF Clone

CAT#: RC215764

SLC6A20 (Myc-DDK-tagged)-Human solute carrier family 6 (proline IMINO transporter), member 20 (SLC6A20), transcript variant 1

Product Images

Circular map for RC215764

• 

  Western blot validation of overexpression lysate (Cat# LY412593) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215764 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC6A20
• Synonyms: IMINO; SIT1; XT3; Xtrp3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215764 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAAGCGCGGCCGCTGTGGGCCAACTCGCTACAGTTCGTGTTCGCCTGCATCTCGTACGCCGTGG
  GCCTGGGCAACGTGTGGCGATTCCCGTACCTGTGCCAGATGTACGGCGGAGGTAGTTTCCTGGTCCCCTA
  CATCATCATGCTTATCGTGGAGGGAATGCCGCTCTTGTACCTGGAACTGGCTGTGGGGCAGCGCATGCGG
  CAGGGCAGCATCGGCGCCTGGAGGACCATCAGCCCGTACCTCAGTGGTGTCGGGGTCGCCAGCGTGGTGG
  TCTCTTTCTTCCTCTCCATGTACTACAACGTCATCAACGCCTGGGCCTTCTGGTACCTCTTCCACTCCTT
  CCAGGATCCCCTGCCGTGGTCTGTCTGCCCACTGAATGGTAACCACACGGGCTACGATGAGGAGTGCGAG
  AAGGCGTCCTCCACACAGTACTTCTGGTACAGGAAAACCCTCAATATCTCGCCGTCCCTCCAGGAGAACG
  GGGGTGTGCAGTGGGAGCCGGCGCTGTGCCTCCTCCTGGCCTGGCTGGTGGTGTACCTGTGCATCCTGCG
  TGGCACCGAGTCCACTGGCAAGGTGGTGTATTTCACGGCGTCACTGCCCTATTGCGTGCTCATCATCTAC
  CTCATCAGGGGCCTCACGCTCCACGGAGCCACCAATGGCCTCATGTACATGTTCACTCCCAAGATAGAGC
  AGCTGGCCAACCCCAAGGCCTGGATCAATGCAGCCACCCAGATCTTCTTCTCACTTGGCCTGGGCTTCGG
  CAGCCTGATCGCCTTCGCCAGCTACAATGAGCCATCCAACAACTGCCAGAAGCACGCCATCATCGTGTCC
  CTCATCAACAGCTTCACCTCCATATTTGCCAGCATTGTCACCTTCTCCATCTATGGCTTCAAGGCCACCT
  TCAATTATGAAAACTGCTTGAAGAAGGTGAGTCTGCTGCTGACCAACACTTTTGACCTTGAAGATGGCTT
  TTTGACAGCCAGCAACCTGGAGCAGGTGAAGGGCTACCTCGCATCTGCCTACCCAAGCAAATACAGCGAG
  ATGTTCCCGCAAATCAAAAACTGCAGCTTGGAATCGGAGCTAGACACGGCCGTCCAGGGCACTGGCCTGG
  CATTCATCGTCTATACAGAGGCCATTAAAAACATGGAGGTGTCCCAGCTGTGGTCGGTGCTCTACTTCTT
  CATGCTGCTGATGCTGGGCATTGGGAGCATGCTGGGGAACACAGCGGCCATCCTCACCCCTCTGACAGAC
  AGCAAGATCATCTCCAGCCACCTGCCCAAGGAGGCCATCTCAGGTCTGGTGTGCCTTGTCAACTGTGCCA
  TTGGCATGGTGTTCACGATGGAGGCTGGGAACTACTGGTTTGACATATTCAACGACTACGCGGCCACACT
  GTCCCTGCTGCTCATCGTGCTGGTGGAGACGATTGCCGTGTGCTACGTGTACGGGCTGAGGAGATTTGAA
  AGTGACCTTAAGGCCATGACCGGCCGAGCTGTGAGCTGGTACTGGAAGGTGATGTGGGCTGGCGTAAGCC
  CACTGCTGATTGTCAGCCTCTTTGTCTTCTACCTGAGCGACTACATCCTCACGGGGACCCTGAAGTATCA
  AGCCTGGGACGCCTCCCAGGGCCAGCTCGTGACCAAAGATTACCCGGCCTATGCACTGGCTGTCATCGGG
  CTGCTTGTGGCCTCCTCCACCATGTGCATCCCCCTGGCGGCCCTGGGGACTTTTGTTCAGCGTCGCCTCA
  AGAGGGGAGACGCAGACCCCGTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215764 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKARPLWANSLQFVFACISYAVGLGNVWRFPYLCQMYGGGSFLVPYIIMLIVEGMPLLYLELAVGQRMR
  QGSIGAWRTISPYLSGVGVASVVVSFFLSMYYNVINAWAFWYLFHSFQDPLPWSVCPLNGNHTGYDEECE
  KASSTQYFWYRKTLNISPSLQENGGVQWEPALCLLLAWLVVYLCILRGTESTGKVVYFTASLPYCVLIIY
  LIRGLTLHGATNGLMYMFTPKIEQLANPKAWINAATQIFFSLGLGFGSLIAFASYNEPSNNCQKHAIIVS
  LINSFTSIFASIVTFSIYGFKATFNYENCLKKVSLLLTNTFDLEDGFLTASNLEQVKGYLASAYPSKYSE
  MFPQIKNCSLESELDTAVQGTGLAFIVYTEAIKNMEVSQLWSVLYFFMLLMLGIGSMLGNTAAILTPLTD
  SKIISSHLPKEAISGLVCLVNCAIGMVFTMEAGNYWFDIFNDYAATLSLLLIVLVETIAVCYVYGLRRFE
  SDLKAMTGRAVSWYWKVMWAGVSPLLIVSLFVFYLSDYILTGTLKYQAWDASQGQLVTKDYPAYALAVIG
  LLVASSTMCIPLAALGTFVQRRLKRGDADPVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020208
• ORF Size: 1776 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020208.4
• RefSeq Size: 5455 bp
• RefSeq ORF: 1779 bp
• Locus ID: 54716
• UniProt ID: Q9NP91
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, Transmembrane
• MW: 65.9 kDa
• Gene Summary: Transport of small hydrophilic substances across cell membranes is mediated by substrate-specific transporter proteins which have been classified into several families of related genes. The protein encoded by this gene belongs to the sodium:neurotransmitter symporter (SNF) family and functions as a proline transporter expressed in kidney and small intestine. Mutations in this gene are associated with Hyperglycinuria and Iminoglycinuria. [provided by RefSeq, Jul 2020]