USP6 (NM_004505) Human Tagged ORF Clone

CAT#: RC215749

USP6 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 6 (Tre-2 oncogene) (USP6)

Product Images

Circular map for RC215749

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP6
• Synonyms: HRP1; Tre-2; TRE2; TRE17; TRESMCR; USP6-short
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215749 representing NM_004505
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATGGTAGAGAATGCAGATAGTTTGCAGGCACAGGAGCGGAAGGACATACTTATGAAGTATGACA
  AGGGACACCGAGCTGGGCTGCCAGAGGACAAGGGGCCTGAGCCCGTTGGAATCAACAGCAGCATTGATCG
  TTTTGGCATTTTGCATGAGACGGAGCTGCCTCCTGTGACTGCACGGGAGGCGAAGAAAATTCGGCGGGAG
  ATGACACGAACGAGCAAGTGGATGGAAATGCTGGGAGAATGGGAGACATATAAGCACAGTAGCAAACTCA
  TAGATCGAGTGTACAAGGGAATTCCCATGAACATCCGGGGCCCGGTGTGGTCAGTCCTCCTGAACATTCA
  GGAAATCAAGTTGAAAAACCCCGGAAGATACCAGATCATGAAGGAGAGGGGCAAGAGGTCATCTGAACAC
  ATCCACCACATCGACCTGGACGTGAGGACGACTCTCCGGAACCATGTCTTCTTTAGGGATCGATATGGAG
  CCAAGCAGAGGGAACTATTCTACATCCTCCTGGCCTATTCGGAGTATAACCCGGAGGTGGGCTACTGCAG
  GGACCTGAGCCACATCACCGCCTTGTTCCTCCTTTATCTGCCTGAGGAGGACGCATTCTGGGCACTGGTG
  CAGCTGCTGGCCAGTGAGAGGCACTCCCTGCCAGGATTCCACAGCCCAAATGGTGGGACAGTCCAGGGGC
  TCCAAGACCAACAGGAGCATGTGGTACCCAAGTCACAACCCAAGACCATGTGGCATCAGGACAAGGAAGG
  TCTATGCGGGCAGTGTGCCTCGTTAGGCTGCCTTCTCCGGAACCTGATTGACGGGATCTCTCTCGGGCTC
  ACCCTGCGCCTGTGGGACGTGTATTTGGTGGAAGGAGAACAGGTGTTGATGCCAATAACCAGCATTGCTC
  TTAAGGTTCAGCAGAAGCGCCTCATGAAGACATCCAGGTGTGGCCTGTGGGCACGTCTGCGGAACCAATT
  CTTCGATACCTGGGCCATGAACGATGACACCGTGCTCAAGCATCTTAGGGCCTCTACGAAGAAACTAACA
  AGGAAGCAAGGGGACCTGCCACCCCCAGCCAAACGCGAGCAAGGGTCCTTGGCACCCAGGCCTGTGCCGG
  CTTCACGTGGTGGGAAGACCCTCTGCAAGGGGTATAGGCAGGCCCCTCCAGGCCCACCAGCCCAGTTCCA
  GCGGCCCATTTGCTCAGCTTCCCCGCCATGGGCATCTCGTTTTTCCACGCCCTGTCCTGGTGGGGCTGTC
  CGGGAAGACACGTACCCTGTGGGCACTCAGGGTGTGCCCAGCCTGGCCCTGGCTCAGGGAGGACCTCAGG
  GTTCCTGGAGATTCCTGGAGTGGAAGTCAATGCCCCGGCTCCCAACGGACCTGGATATAGGGGGCCCTTG
  GTTCCCCCATTATGATTTTGAATGGAGCTGCTGGGTCCGTGCCATATCCCAGGAGGACCAGCTGGCCACC
  TGCTGGCAGGCTGAACACTGCGGAGAGGTTCACAACAAAGATATGAGTTGGCCTGAGGAGATGTCTTTTA
  CAGCAAATAGTAGTAAAATAGATAGACAAAAGGTTCCCACAGAAAAGGGAGCCACAGGTCTAAGCAACCT
  GGGAAACACATGCTTCATGAACTCAAGCATCCAGTGCGTTAGTAACACACAGCCACTGACACAGTATTTT
  ATCTCAGGGAGACATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAAGGGGCATATGGCTAAAT
  GCTATGGTGATTTAGTGCAGGAACTCTGGAGTGGAACTCAGAAGAGTGTTGCCCCATTAAAGCTTCGGCG
  GACCATAGCAAAATATGCTCCCAAGTTTGATGGGTTTCAGCAACAAGACTCCCAAGAACTTCTGGCTTTT
  CTCTTGGATGGTCTTCATGAAGATCTCAACCGAGTCCATGAAAAGCCATATGTGGAACTGAAGGACAGTG
  ATGGCCGACCAGACTGGGAAGTAGCTGCAGAGGCCTGGGACAACCATCTAAGAAGAAATAGATCAATTAT
  TGTGGATTTGTTCCATGGGCAGCTAAGATCTCAAGTCAAATGCAAGACATGTGGGCATATAAGTGTCCGA
  TTTGACCCTTTCAATTTTTTGTCTTTGCCACTACCAATGGACAGTTACATGGACTTAGAAATAACAGTGA
  TTAAGTTAGATGGTACTACCCCTGTACGGTATGGACTAAGACTGAATATGGATGAAAAGTACACAGGTTT
  AAAAAAACAGCTGAGGGATCTCTGTGGACTTAATTCAGAACAAATCCTACTAGCAGAAGTACATGATTCC
  AACATAAAGAACTTTCCTCAGGATAACCAAAAAGTACAACTCTCAGTGAGCGGATTTTTGTGTGCATTTG
  AAATTCCTGTCCCTTCATCTCCAATTTCAGCTTCTAGTCCAACACAAATAGATTTCTCCTCTTCACCATC
  TACAAATGGAATGTTCACCCTAACTACCAATGGGGACCTACCCAAACCAATATTCATCCCCAATGGAATG
  CCAAACACTGTTGTGCCATGTGGAACTGAGAAGAACTTCACAAATGGAATGGTTAATGGTCACATGCCAT
  CTCTTCCTGACAGCCCCTTTACAGGTTACATCATTGCAGTCCACCGAAAAATGATGAGGACAGAACTGTA
  TTTCCTGTCACCTCAGGAGAATCGCCCCAGCCTCTTTGGAATGCCATTGATTGTTCCATGCACTGTGCAT
  ACCCGGAAGAAAGACCTATATGATGCGGTTTGGATTCAAGTATCCTGGTTAGCAAGACCACTCCCACCTC
  AGGAAGCTAGTATTCATGCCCAGGATCGTGATAACTGTATGGGCTATCAATATCCATTCACTCTACGAGT
  TGTGCAGAAAGATGGGAACTCCTGTGCTTGGTGCCCACAGTATAGATTTTGCAGAGGCTGTAAAATTGAT
  TGTGGGGAAGACAGAGCTTTCATTGGAAATGCCTATATTGCTGTGGATTGGCACCCCACAGCCCTTCACC
  TTCGCTATCAAACATCCCAGGAAAGGGTTGTAGATAAGCATGAGAGTGTGGAGCAGAGTCGGCGAGCGCA
  AGCCGAGCCCATCAACCTGGACAGCTGTCTCCGTGCTTTCACCAGTGAGGAAGAGCTAGGGGAAAGTGAG
  ATGTACTACTGTTCCAAGTGTAAGACCCACTGCTTAGCAACAAAGAAGCTGGATCTCTGGAGGCTTCCAC
  CCTTCCTGATTATTCACCTTAAGCGATTTCAATTTGTAAATGATCAGTGGATAAAATCACAGAAAATTGT
  CAGATTTCTTCGGGAAAGTTTTGATCCGAGTGCTTTTTTGGTACCACGAGACCCGGCCCTCTGCCAGCAT
  AAACCACTCACACCCCAGGGGGATGAGCTCTCCAAGCCCAGGATTCTGGCAAGAGAGGTGAAGAAAGTGG
  ATGCGCAGAGTTCGGCTGGAAAAGAGGACATGCTCCTAAGCAAAAGCCCATCCTCACTCAGCGCTAACAT
  CAGCAGCAGCCCAAAAGGTTCTCCTTCTTCATCAAGAAAAAGTGGAACCAGCTGTCCCTCCAGCAAAAAC
  AGCAGCCCTAATAGCAGCCCACGGACTTTGGGGAGGAGCAAAGGGAGGCTCCGGCTGCCCCAGATTGGCA
  GCAAAAATAAGCCGTCAAGTAGTAAGAAGAACTTGGATGCCAGCAAAGAGAATGGGGCTGGGCAGATCTG
  TGAGCTGGCTGACGCCTTGAGCCGAGGGCATATGCGGGGGGGCAGCCAACCAGAGCTGGTCACTCCTCAG
  GACCATGAGGTAGCTTTGGCCAATGGATTCCTTTATGAGCATGAAGCATGTGGCAATGGCTGTGGCGATG
  GCTACAGCAATGGTCAGCTTGGAAACCACAGTGAAGAAGACAGCACTGATGACCAAAGAGAAGACACTCA
  TATTAAGCCTATTTATAATCTATATGCAATTTCATGCCATTCAGGAATTCTGAGTGGGGGCCATTACATC
  ACTTATGCCAAAAACCCAAACTGCAAGTGGTACTGTTATAATGACAGCAGCTGTGAGGAACTTCACCCTG
  ATGAAATTGACACCGACTCTGCCTACATTCTTTTCTATGAGCAGCAGGGGATAGACTACGCACAATTTCT
  GCCAAAGATTGATGGCAAAAAGATGGCAGACACAAGCAGTACGGATGAAGACTCTGAGTCTGATTACGAA
  AAGTACTCTATGTTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215749 representing NM_004505
  Red=Cloning site Green=Tags(s)
  
  MDMVENADSLQAQERKDILMKYDKGHRAGLPEDKGPEPVGINSSIDRFGILHETELPPVTAREAKKIRRE
  MTRTSKWMEMLGEWETYKHSSKLIDRVYKGIPMNIRGPVWSVLLNIQEIKLKNPGRYQIMKERGKRSSEH
  IHHIDLDVRTTLRNHVFFRDRYGAKQRELFYILLAYSEYNPEVGYCRDLSHITALFLLYLPEEDAFWALV
  QLLASERHSLPGFHSPNGGTVQGLQDQQEHVVPKSQPKTMWHQDKEGLCGQCASLGCLLRNLIDGISLGL
  TLRLWDVYLVEGEQVLMPITSIALKVQQKRLMKTSRCGLWARLRNQFFDTWAMNDDTVLKHLRASTKKLT
  RKQGDLPPPAKREQGSLAPRPVPASRGGKTLCKGYRQAPPGPPAQFQRPICSASPPWASRFSTPCPGGAV
  REDTYPVGTQGVPSLALAQGGPQGSWRFLEWKSMPRLPTDLDIGGPWFPHYDFEWSCWVRAISQEDQLAT
  CWQAEHCGEVHNKDMSWPEEMSFTANSSKIDRQKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYF
  ISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKSVAPLKLRRTIAKYAPKFDGFQQQDSQELLAF
  LLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIIVDLFHGQLRSQVKCKTCGHISVR
  FDPFNFLSLPLPMDSYMDLEITVIKLDGTTPVRYGLRLNMDEKYTGLKKQLRDLCGLNSEQILLAEVHDS
  NIKNFPQDNQKVQLSVSGFLCAFEIPVPSSPISASSPTQIDFSSSPSTNGMFTLTTNGDLPKPIFIPNGM
  PNTVVPCGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSPQENRPSLFGMPLIVPCTVH
  TRKKDLYDAVWIQVSWLARPLPPQEASIHAQDRDNCMGYQYPFTLRVVQKDGNSCAWCPQYRFCRGCKID
  CGEDRAFIGNAYIAVDWHPTALHLRYQTSQERVVDKHESVEQSRRAQAEPINLDSCLRAFTSEEELGESE
  MYYCSKCKTHCLATKKLDLWRLPPFLIIHLKRFQFVNDQWIKSQKIVRFLRESFDPSAFLVPRDPALCQH
  KPLTPQGDELSKPRILAREVKKVDAQSSAGKEDMLLSKSPSSLSANISSSPKGSPSSSRKSGTSCPSSKN
  SSPNSSPRTLGRSKGRLRLPQIGSKNKPSSSKKNLDASKENGAGQICELADALSRGHMRGGSQPELVTPQ
  DHEVALANGFLYEHEACGNGCGDGYSNGQLGNHSEEDSTDDQREDTHIKPIYNLYAISCHSGILSGGHYI
  TYAKNPNCKWYCYNDSSCEELHPDEIDTDSAYILFYEQQGIDYAQFLPKIDGKKMADTSSTDEDSESDYE
  KYSMLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004505
• ORF Size: 4218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004505.4
• RefSeq Size: 7971 bp
• RefSeq ORF: 4221 bp
• Locus ID: 9098
• UniProt ID: P35125
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome, Protease
• MW: 158.5 kDa
• Gene Summary: Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.[UniProtKB/Swiss-Prot Function]