KCNK7 (NM_033455) Human Tagged ORF Clone

CAT#: RC215648

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KCNK7 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 7 (KCNK7), transcript variant D


  "NM_033455" in other vectors (4)

USD 98.00

USD 390.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name KCNK7 (NM_033455) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KCNK7
Synonyms K2p7.1; TWIK3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC215648 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGGTCTAAGGCCCTGGTCCCGATACGGGCTCCTGGTTGTGGCCCACTTGCTGGCCCTGGGGCTTG
GGGCTGTGGTGTTCCAGGCCCTGGAGGGGCCTCCTGCATGCAGGCTTCAGGCTGAGCTCAGGGCAGAGCT
GGCAGCCTTCCAGGCAGAGCATAGGGCCTGCCTGCCACCCGGAGCTCTGGAAGAGCTGCTGGGCACTGCC
CTGGCCACCCAGGCCCATGGGGTCTCCACCCTGGGCAACAGCTCAGAGGGCAGGACCTGGGACCTTCCCT
CAGCCCTGCTCTTCGCTGCCAGCATCCTCACCACCACAGGTTATGGCCACATGGCCCCACTATCGCCAGG
CGGAAAGGCCTTCTGCATGGTCTATGCAGCCCTGGGGCTGCCAGCCTCCTTAGCTCTCGTGGCCACCCTG
CGCCATTGCCTGCTGCCTGTGCTCAGCCGCCCACGTGCCTGGGTAGCGGTCCACTGGCAGCTGTCACCGG
CCAGCGCTGCGCTGCTGCAGGCAGTTGCACTGGGACTGCTGGTGGCCAGCAGCTTTGTGCTGCTGCCAGC
GCTGGTGCTGTGGGGCCTTCAGGGCGACTGCAGCCTGCTGGGGGCCGTCTACTTCTGCTTCAGCTCGCTC
AGCACCATTGGCCTGGAGGACTTGCTGCCCGGCCGCGGCCGCAGCCTGCACCCCGTGATTTACCACCTGG
GCCAGCTCGCACTTCTTGGTGGAGGGACCTCACTCCAGGGCACGGCGTGGGAGGGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC215648 protein sequence
Red=Cloning site Green=Tags(s)

MGGLRPWSRYGLLVVAHLLALGLGAVVFQALEGPPACRLQAELRAELAAFQAEHRACLPPGALEELLGTA
LATQAHGVSTLGNSSEGRTWDLPSALLFAASILTTTGYGHMAPLSPGGKAFCMVYAALGLPASLALVATL
RHCLLPVLSRPRAWVAVHWQLSPASAALLQAVALGLLVASSFVLLPALVLWGLQGDCSLLGAVYFCFSSL
STIGLEDLLPGRGRSLHPVIYHLGQLALLGGGTSLQGTAWEG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_033455
ORF Size 756 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033455.1, NP_258416.1
RefSeq Size 1485
RefSeq ORF 759
Locus ID 10089
Cytogenetics 11q13.1
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
MW 26.1 kDa
Gene Summary This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel; however, it may require other non-pore-forming proteins for activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Other Versions

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