ROR2 (NM_004560) Human Tagged ORF Clone

CAT#: RC215640

ROR2 (Myc-DDK-tagged)-Human receptor tyrosine kinase-like orphan receptor 2 (ROR2)

Product Images

Circular map for RC215640

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ROR2 (Cat# RC215640, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROR2 (Cat# TA810020)(1:2000). Positive lysates LY417906 (100ug) and LC417906 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY417906) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215640 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ROR2 protein (Cat# TP315640). The protein was produced from HEK293T cells transfected with ROR2 cDNA clone (Cat# RC215640) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ROR2
• Synonyms: BDB; BDB1; NTRKR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215640 representing NM_004560
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGGGCTCGGCGCTCCCGCGGCGGCCGCTGCTGTGCATCCCGGCCGTCTGGGCGGCCGCCGCGC
  TTCTGCTCTCAGTGTCCCGGACTTCAGGTGAAGTGGAGGTTCTGGATCCGAACGACCCTTTAGGACCCCT
  TGATGGGCAGGACGGCCCGATTCCAACTCTGAAAGGTTACTTTCTGAATTTTCTGGAGCCAGTAAACAAT
  ATCACCATTGTCCAAGGCCAGACGGCAATTCTGCACTGCAAGGTGGCAGGAAACCCACCCCCTAACGTGC
  GGTGGCTAAAGAATGATGCCCCGGTGGTGCAGGAGCCGCGGCGGATCATCATCCGGAAGACAGAATATGG
  TTCACGACTGCGAATCCAGGACCTGGACACGACAGACACTGGCTACTACCAGTGCGTGGCCACCAACGGG
  ATGAAGACCATTACCGCCACTGGCGTCCTGTTTGTGCGGCTGGGTCCAACGCACAGCCCAAATCATAACT
  TTCAGGATGATTACCACGAGGATGGGTTCTGCCAGCCTTACCGGGGAATTGCCTGTGCACGCTTCATTGG
  CAACCGGACCATTTATGTGGACTCGCTTCAGATGCAGGGGGAGATTGAAAACCGAATCACAGCGGCCTTC
  ACCATGATCGGCACGTCTACGCACCTGTCGGACCAGTGCTCACAGTTCGCCATCCCATCCTTCTGCCACT
  TCGTGTTTCCTCTGTGCGACGCGCGCTCCCGGGCACCCAAGCCGCGTGAGCTGTGCCGCGACGAGTGCGA
  GGTGCTGGAGAGCGACCTGTGCCGCCAGGAGTACACCATCGCCCGCTCCAACCCGCTCATCCTCATGCGG
  CTTCAGCTGCCCAAGTGTGAGGCGCTGCCCATGCCTGAGAGCCCCGACGCTGCCAACTGCATGCGCATTG
  GCATCCCAGCCGAGAGGCTGGGCCGCTACCATCAGTGCTATAACGGCTCAGGCATGGATTACAGAGGAAC
  GGCAAGCACCACCAAGTCAGGCCACCAGTGCCAGCCGTGGGCCCTGCAGCACCCCCACAGCCACCACCTG
  TCCAGCACAGACTTCCCTGAGCTTGGAGGGGGGCACGCCTACTGCCGGAACCCCGGAGGCCAGATGGAGG
  GCCCCTGGTGCTTTACGCAGAATAAAAACGTACGCATGGAACTGTGTGACGTACCCTCGTGTAGTCCCCG
  AGACAGCAGCAAGATGGGGATTCTGTACATCTTGGTCCCCAGCATCGCAATTCCACTGGTCATCGCTTGC
  CTTTTCTTCTTGGTTTGCATGTGCCGGAATAAGCAGAAGGCATCTGCGTCCACACCGCAGCGGCGACAGC
  TGATGGCCTCGCCCAGCCAAGACATGGAAATGCCCCTCATTAACCAGCACAAACAGGCCAAACTCAAAGA
  GATCAGCCTGTCTGCGGTGAGGTTCATGGAGGAGCTGGGAGAGGACCGGTTTGGGAAAGTCTACAAAGGT
  CACCTGTTCGGCCCTGCCCCGGGGGAGCAGACCCAGGCTGTGGCCATCAAAACGCTGAAGGACAAAGCGG
  AGGGGCCCCTGCGGGAGGAGTTCCGGCATGAGGCTATGCTGCGAGCACGGCTGCAACACCCCAACGTCGT
  CTGCCTGCTGGGCGTGGTGACCAAGGACCAGCCCCTGAGCATGATCTTCAGCTACTGTTCGCACGGCGAC
  CTCCACGAATTCCTGGTCATGCGCTCGCCGCACTCGGACGTGGGCAGCACCGATGATGACCGCACGGTGA
  AGTCCGCCCTGGAGCCCCCCGACTTCGTGCACCTTGTGGCACAGATCGCGGCGGGGATGGAGTACCTATC
  CAGCCACCACGTGGTTCACAAGGACCTGGCCACCCGCAATGTGCTAGTGTACGACAAGCTGAACGTGAAG
  ATCTCAGACTTGGGCCTCTTCCGAGAGGTGTATGCCGCCGATTACTACAAGCTGCTGGGGAACTCGCTGC
  TGCCTATCCGCTGGATGGCCCCAGAGGCCATCATGTACGGCAAGTTCTCCATCGACTCAGACATCTGGTC
  CTACGGTGTGGTCCTGTGGGAGGTCTTCAGCTACGGCCTGCAGCCCTACTGCGGGTATTCCAACCAGGAT
  GTGGTGGAGATGATCCGGAACCGGCAGGTGCTGCCTTGCCCCGATGACTGTCCCGCCTGGGTGTATGCCC
  TCATGATCGAGTGCTGGAACGAGTTCCCCAGCCGGCGGCCCCGCTTCAAGGACATCCACAGCCGGCTCCG
  AGCCTGGGGCAACCTTTCCAACTACAACAGCTCGGCGCAGACCTCGGGGGCCAGCAACACCACGCAGACC
  AGCTCCCTGAGCACCAGCCCAGTGAGCAATGTGAGCAACGCCCGCTACGTGGGGCCCAAGCAGAAGGCCC
  CGCCCTTCCCACAGCCCCAGTTCATCCCCATGAAGGGCCAGATCAGACCCATGGTGCCCCCGCCGCAGCT
  CTACATCCCCGTCAACGGCTACCAGCCGGTGCCGGCCTATGGGGCCTACCTGCCCAACTTCTACCCGGTG
  CAGATCCCAATGCAGATGGCCCCGCAGCAGGTGCCTCCTCAGATGGTCCCCAAGCCCAGCTCACACCACA
  GTGGCAGTGGCTCCACCAGCACAGGCTACGTCACCACGGCCCCCTCCAACACATCCATGGCAGACAGGGC
  AGCCCTGCTCTCAGAGGGCGCTGATGACACACAGAACGCCCCAGAAGATGGGGCCCAGAGCACCGTGCAG
  GAAGCAGAGGAGGAGGAGGAAGGCTCTGTCCCAGAGACTGAGCTGCTGGGGGACTGTGACACTCTGCAGG
  TGGACGAGGCCCAAGTCCAGCTGGAAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215640 representing NM_004560
  Red=Cloning site Green=Tags(s)
  
  MARGSALPRRPLLCIPAVWAAAALLLSVSRTSGEVEVLDPNDPLGPLDGQDGPIPTLKGYFLNFLEPVNN
  ITIVQGQTAILHCKVAGNPPPNVRWLKNDAPVVQEPRRIIIRKTEYGSRLRIQDLDTTDTGYYQCVATNG
  MKTITATGVLFVRLGPTHSPNHNFQDDYHEDGFCQPYRGIACARFIGNRTIYVDSLQMQGEIENRITAAF
  TMIGTSTHLSDQCSQFAIPSFCHFVFPLCDARSRAPKPRELCRDECEVLESDLCRQEYTIARSNPLILMR
  LQLPKCEALPMPESPDAANCMRIGIPAERLGRYHQCYNGSGMDYRGTASTTKSGHQCQPWALQHPHSHHL
  SSTDFPELGGGHAYCRNPGGQMEGPWCFTQNKNVRMELCDVPSCSPRDSSKMGILYILVPSIAIPLVIAC
  LFFLVCMCRNKQKASASTPQRRQLMASPSQDMEMPLINQHKQAKLKEISLSAVRFMEELGEDRFGKVYKG
  HLFGPAPGEQTQAVAIKTLKDKAEGPLREEFRHEAMLRARLQHPNVVCLLGVVTKDQPLSMIFSYCSHGD
  LHEFLVMRSPHSDVGSTDDDRTVKSALEPPDFVHLVAQIAAGMEYLSSHHVVHKDLATRNVLVYDKLNVK
  ISDLGLFREVYAADYYKLLGNSLLPIRWMAPEAIMYGKFSIDSDIWSYGVVLWEVFSYGLQPYCGYSNQD
  VVEMIRNRQVLPCPDDCPAWVYALMIECWNEFPSRRPRFKDIHSRLRAWGNLSNYNSSAQTSGASNTTQT
  SSLSTSPVSNVSNARYVGPKQKAPPFPQPQFIPMKGQIRPMVPPPQLYIPVNGYQPVPAYGAYLPNFYPV
  QIPMQMAPQQVPPQMVPKPSSHHSGSGSTSTGYVTTAPSNTSMADRAALLSEGADDTQNAPEDGAQSTVQ
  EAEEEEEGSVPETELLGDCDTLQVDEAQVQLEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004560
• ORF Size: 2829 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_004560.4
• RefSeq Size: 4091 bp
• RefSeq ORF: 2832 bp
• Locus ID: 4920
• UniProt ID: Q01974
• Cytogenetics: 9q22.31
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• MW: 104.74 kDa
• Gene Summary: The protein encoded by this gene is a receptor protein tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. The protein may be involved in the early formation of the chondrocytes and may be required for cartilage and growth plate development. Mutations in this gene can cause brachydactyly type B, a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails. In addition, mutations in this gene can cause the autosomal recessive form of Robinow syndrome, which is characterized by skeletal dysplasia with generalized limb bone shortening, segmental defects of the spine, brachydactyly, and a dysmorphic facial appearance. [provided by RefSeq, Jul 2008]