AMELX (NM_182680) Human Tagged ORF Clone

CAT#: RC215582

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AMELX (Myc-DDK-tagged)-Human amelogenin, X-linked (AMELX), transcript variant 3



  "NM_182680" in other vectors (6)

USD 98.00

USD 390.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Product Name AMELX (NM_182680) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol AMELX
Synonyms AI1E; AIH1; ALGN; AMG; AMGL; AMGX
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC215582 representing NM_182680
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGACCTGGATTTTATTTGCCTGCCTCCTGGGAGCAGCTTTTGCCATGCCTCTACCACCTCATCCTG
GGCACCCTGGTTATATCAACTTCAGCTATGAGAACTCACATTCTCAGGCTATCAATGTTGACAGGACTGC
ATTAGTGCTTACCCCTTTGAAGTGGTACCAGAGCATAAGGCCACCGTACCCTTCCTATGGTTACGAGCCC
ATGGGTGGATGGCTGCACCACCAAATCATCCCCGTGCTGTCCCAACAGCACCCCCCGACTCACACCCTGC
AGCCTCATCACCACATCCCAGTGGTGCCAGCTCAGCAGCCCGTGATCCCCCAGCAACCAATGATGCCCGT
TCCTGGCCAACACTCCATGACTCCAATCCAACACCACCAGCCAAACCTCCCTCCGCCCGCCCAGCAGCCC
TACCAGCCCCAGCCTGTTCAGCCACAGCCTCACCAGCCCATGCAGCCCCAGCCACCTGTGCACCCCATGC
AGCCCCTGCCGCCACAGCCACCTCTGCCTCCGATGTTCCCCATGCAGCCCCTGCCTCCCATGCTTCCTGA
TCTGACTCTGGAAGCTTGGCCATCAACAGACAAGACCAAGCGGGAGGAAGTGGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC215582 representing NM_182680
Red=Cloning site Green=Tags(s)

MGTWILFACLLGAAFAMPLPPHPGHPGYINFSYENSHSQAINVDRTALVLTPLKWYQSIRPPYPSYGYEP
MGGWLHHQIIPVLSQQHPPTHTLQPHHHIPVVPAQQPVIPQQPMMPVPGQHSMTPIQHHQPNLPPPAQQP
YQPQPVQPQPHQPMQPQPPVHPMQPLPPQPPLPPMFPMQPLPPMLPDLTLEAWPSTDKTKREEVD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_182680
ORF Size 615 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_182680.1, NP_872621.1
RefSeq Size 835 bp
RefSeq ORF 618 bp
Locus ID 265
Cytogenetics Xp22.2
Protein Families Druggable Genome, Secreted Protein, Transmembrane
MW 21.4 kDa
Gene Summary This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Citations (1)

Other Versions

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