ASH2L (NM_004674) Human Tagged ORF Clone

CAT#: RC215552

ASH2L (Myc-DDK-tagged)-Human ash2 (absent, small, or homeotic)-like (Drosophila) (ASH2L), transcript variant 1

Product Images

Circular map for RC215552

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ASH2L (Cat# RC215552, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASH2L (Cat# TA810989)(1:2000). Positive lysates LY401483 (100ug) and LC401483 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401483) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215552 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ASH2L protein (Cat# TP315552). The protein was produced from HEK293T cells transfected with ASH2L cDNA clone (Cat# RC215552) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASH2L
• Synonyms: ASH2; ASH2L1; ASH2L2; Bre2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215552 representing NM_004674
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCAGGAGCAGGACCTGGCCAGGAAGCGGGTGCCGGGCCTGGCCCAGGAGCGGTCGCAAATG
  CAACAGGGGCAGAAGAGGGGGAGATGAAGCCGGTGGCAGCGGGAGCAGCCGCTCCTCCTGGAGAGGGGAT
  CTCTGCTGCTCCGACAGTTGAGCCCAGTTCCGGGGAGGCTGAAGGCGGGGAGGCAAACTTGGTCGATGTA
  AGCGGTGGCTTGGAGACAGAATCATCTAATGGAAAAGATACACTAGAAGGTGCTGGGGATACATCAGAGG
  TGATGGATACTCAGGCGGGCTCCGTGGATGAAGAGAATGGCCGACAGTTGGGTGAGGTAGAGCTGCAATG
  TGGGATTTGTACAAAATGGTTCACGGCTGACACATTTGGCATAGATACCTCATCCTGTCTACCTTTCATG
  ACCAACTACAGTTTTCATTGCAACGTCTGCCATCACAGTGGGAATACCTATTTCCTCCGGAAGCAAGCAA
  ACTTGAAGGAAATGTGCCTTAGTGCTTTGGCCAACCTGACATGGCAGTCCCGAACACAGGATGAACATCC
  GAAGACAATGTTCTCCAAAGATAAGGATATTATACCATTTATTGATAAATACTGGGAGTGCATGACAACC
  AGACAGAGACCTGGGAAAATGACTTGGCCAAATAACATTGTTAAAACAATGAGTAAAGAAAGAGATGTAT
  TCTTGGTAAAGGAACACCCAGATCCAGGCAGTAAAGATCCAGAAGAAGATTACCCCAAATTTGGACTTTT
  GGATCAGGACCTTAGTAACATTGGTCCTGCTTATGACAACCAAAAACAGAGCAGTGCTGTGTCTACTAGT
  GGGAATTTAAATGGGGGAATTGCAGCAGGAAGCAGCGGAAAAGGACGAGGAGCCAAGCGCAAACAGCAGG
  ATGGAGGGACCACAGGGACCACCAAGAAGGCCCGGAGTGACCCTTTGTTTTCTGCTCAGCGCCTTCCCCC
  TCATGGCTACCCATTGGAACACCCGTTTAACAAAGATGGCTATCGGTATATTCTAGCTGAGCCTGATCCG
  CACGCCCCTGACCCCGAGAAGCTGGAACTTGACTGCTGGGCAGGAAAACCTATTCCTGGAGACCTCTACA
  GAGCCTGCTTGTATGAACGGGTTTTGTTAGCCCTACATGATCGAGCTCCCCAGTTAAAGATCTCAGATGA
  CCGGCTGACTGTGGTTGGAGAGAAGGGCTACTCTATGGTGAGGGCCTCTCATGGAGTACGGAAGGGTGCC
  TGGTATTTTGAAATCACTGTGGATGAGATGCCACCAGATACCGCTGCCAGACTGGGTTGGTCCCAGCCCC
  TAGGAAACCTTCAAGCTCCTTTAGGTTATGATAAATTTAGCTATTCTTGGCGGAGCAAAAAGGGAACCAA
  GTTCCACCAGTCCATTGGCAAACACTACTCTTCTGGCTATGGACAGGGAGACGTCCTGGGATTTTATATT
  AATCTTCCTGAAGACACAGAGACAGCCAAGTCATTGCCAGACACATACAAAGATAAGGCTTTGATAAAAT
  TCAAGAGTTATTTGTATTTTGAGGAAAAAGACTTTGTGGATAAAGCAGAGAAGAGCCTGAAGCAGACTCC
  CCATAGTGAGATAATATTTTATAAAAATGGTGTCAATCAAGGTGTGGCTTACAAAGATATTTTTGAGGGG
  GTTTACTTCCCAGCCATCTCACTGTACAAGAGCTGCACGGTTTCCATTAACTTTGGACCATGCTTCAAGT
  ATCCTCCGAAGGATCTCACTTACCGCCCTATGAGTGACATGGGCTGGGGCGCCGTGGTAGAGCACACCCT
  GGCTGACGTCTTGTATCACGTGGAGACAGAAGTGGATGGGAGGCGCAGTCCCCCATGGGAACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215552 representing NM_004674
  Red=Cloning site Green=Tags(s)
  
  MAAAGAGPGQEAGAGPGPGAVANATGAEEGEMKPVAAGAAAPPGEGISAAPTVEPSSGEAEGGEANLVDV
  SGGLETESSNGKDTLEGAGDTSEVMDTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFM
  TNYSFHCNVCHHSGNTYFLRKQANLKEMCLSALANLTWQSRTQDEHPKTMFSKDKDIIPFIDKYWECMTT
  RQRPGKMTWPNNIVKTMSKERDVFLVKEHPDPGSKDPEEDYPKFGLLDQDLSNIGPAYDNQKQSSAVSTS
  GNLNGGIAAGSSGKGRGAKRKQQDGGTTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDP
  HAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGA
  WYFEITVDEMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYI
  NLPEDTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEG
  VYFPAISLYKSCTVSINFGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004674
• ORF Size: 1884 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004674.5
• RefSeq Size: 2381 bp
• RefSeq ORF: 1887 bp
• Locus ID: 9070
• UniProt ID: Q9UBL3
• Cytogenetics: 8p11.23
• Domains: SPRY
• Protein Families: Druggable Genome, Transcription Factors
• MW: 68.5 kDa
• Gene Summary: Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.[UniProtKB/Swiss-Prot Function]