ASH2L (NM_004674) Human Tagged ORF Clone

CAT#: RC215552

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ASH2L (Myc-DDK-tagged)-Human ash2 (absent, small, or homeotic)-like (Drosophila) (ASH2L), transcript variant 1



  "NM_004674" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 847.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ASH2L
Synonyms ASH2; ASH2L1; ASH2L2; Bre2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC215552 representing NM_004674
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCAGGAGCAGGACCTGGCCAGGAAGCGGGTGCCGGGCCTGGCCCAGGAGCGGTCGCAAATG
CAACAGGGGCAGAAGAGGGGGAGATGAAGCCGGTGGCAGCGGGAGCAGCCGCTCCTCCTGGAGAGGGGAT
CTCTGCTGCTCCGACAGTTGAGCCCAGTTCCGGGGAGGCTGAAGGCGGGGAGGCAAACTTGGTCGATGTA
AGCGGTGGCTTGGAGACAGAATCATCTAATGGAAAAGATACACTAGAAGGTGCTGGGGATACATCAGAGG
TGATGGATACTCAGGCGGGCTCCGTGGATGAAGAGAATGGCCGACAGTTGGGTGAGGTAGAGCTGCAATG
TGGGATTTGTACAAAATGGTTCACGGCTGACACATTTGGCATAGATACCTCATCCTGTCTACCTTTCATG
ACCAACTACAGTTTTCATTGCAACGTCTGCCATCACAGTGGGAATACCTATTTCCTCCGGAAGCAAGCAA
ACTTGAAGGAAATGTGCCTTAGTGCTTTGGCCAACCTGACATGGCAGTCCCGAACACAGGATGAACATCC
GAAGACAATGTTCTCCAAAGATAAGGATATTATACCATTTATTGATAAATACTGGGAGTGCATGACAACC
AGACAGAGACCTGGGAAAATGACTTGGCCAAATAACATTGTTAAAACAATGAGTAAAGAAAGAGATGTAT
TCTTGGTAAAGGAACACCCAGATCCAGGCAGTAAAGATCCAGAAGAAGATTACCCCAAATTTGGACTTTT
GGATCAGGACCTTAGTAACATTGGTCCTGCTTATGACAACCAAAAACAGAGCAGTGCTGTGTCTACTAGT
GGGAATTTAAATGGGGGAATTGCAGCAGGAAGCAGCGGAAAAGGACGAGGAGCCAAGCGCAAACAGCAGG
ATGGAGGGACCACAGGGACCACCAAGAAGGCCCGGAGTGACCCTTTGTTTTCTGCTCAGCGCCTTCCCCC
TCATGGCTACCCATTGGAACACCCGTTTAACAAAGATGGCTATCGGTATATTCTAGCTGAGCCTGATCCG
CACGCCCCTGACCCCGAGAAGCTGGAACTTGACTGCTGGGCAGGAAAACCTATTCCTGGAGACCTCTACA
GAGCCTGCTTGTATGAACGGGTTTTGTTAGCCCTACATGATCGAGCTCCCCAGTTAAAGATCTCAGATGA
CCGGCTGACTGTGGTTGGAGAGAAGGGCTACTCTATGGTGAGGGCCTCTCATGGAGTACGGAAGGGTGCC
TGGTATTTTGAAATCACTGTGGATGAGATGCCACCAGATACCGCTGCCAGACTGGGTTGGTCCCAGCCCC
TAGGAAACCTTCAAGCTCCTTTAGGTTATGATAAATTTAGCTATTCTTGGCGGAGCAAAAAGGGAACCAA
GTTCCACCAGTCCATTGGCAAACACTACTCTTCTGGCTATGGACAGGGAGACGTCCTGGGATTTTATATT
AATCTTCCTGAAGACACAGAGACAGCCAAGTCATTGCCAGACACATACAAAGATAAGGCTTTGATAAAAT
TCAAGAGTTATTTGTATTTTGAGGAAAAAGACTTTGTGGATAAAGCAGAGAAGAGCCTGAAGCAGACTCC
CCATAGTGAGATAATATTTTATAAAAATGGTGTCAATCAAGGTGTGGCTTACAAAGATATTTTTGAGGGG
GTTTACTTCCCAGCCATCTCACTGTACAAGAGCTGCACGGTTTCCATTAACTTTGGACCATGCTTCAAGT
ATCCTCCGAAGGATCTCACTTACCGCCCTATGAGTGACATGGGCTGGGGCGCCGTGGTAGAGCACACCCT
GGCTGACGTCTTGTATCACGTGGAGACAGAAGTGGATGGGAGGCGCAGTCCCCCATGGGAACCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC215552 representing NM_004674
Red=Cloning site Green=Tags(s)

MAAAGAGPGQEAGAGPGPGAVANATGAEEGEMKPVAAGAAAPPGEGISAAPTVEPSSGEAEGGEANLVDV
SGGLETESSNGKDTLEGAGDTSEVMDTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFM
TNYSFHCNVCHHSGNTYFLRKQANLKEMCLSALANLTWQSRTQDEHPKTMFSKDKDIIPFIDKYWECMTT
RQRPGKMTWPNNIVKTMSKERDVFLVKEHPDPGSKDPEEDYPKFGLLDQDLSNIGPAYDNQKQSSAVSTS
GNLNGGIAAGSSGKGRGAKRKQQDGGTTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDP
HAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGA
WYFEITVDEMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYI
NLPEDTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEG
VYFPAISLYKSCTVSINFGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004674
ORF Size 1884 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004674.5
RefSeq Size 2381 bp
RefSeq ORF 1887 bp
Locus ID 9070
UniProt ID Q9UBL3
Cytogenetics 8p11.23
Domains SPRY
Protein Families Druggable Genome, Transcription Factors
MW 68.5 kDa
Gene Summary Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.[UniProtKB/Swiss-Prot Function]

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