STK23 (SRPK3) (NM_014370) Human Tagged ORF Clone

CAT#: RC215496

SRPK3 (Myc-DDK-tagged)-Human SRSF protein kinase 3 (SRPK3), transcript variant 1

Product Images

Circular map for RC215496

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SRPK3 (Cat# RC215496, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRPK3 (Cat# TA809462)(1:2000). Positive lysates LY415334 (100ug) and LC415334 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY415334) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215496 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SRPK3 protein (Cat# TP315496). The protein was produced from HEK293T cells transfected with SRPK3 cDNA clone (Cat# RC215496) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STK23
• Synonyms: MSSK-1; MSSK1; STK23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215496 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGCGGCAGTAGCAGCAGCTCACAGGCCT
  CCTGCGGGCCCGAGTCCTCGGGCTCCGAACTAGCCCTGGCCACACCGGTGCCTCAGATGCTGCAGGGCCT
  TCTGGGCTCCGACGACGAGGAACAGGAAGACCCCAAAGACTACTGCAAGGGCGGCTACCACCCTGTGAAG
  ATCGGCGACGTGTTCAATGGGCGGTACCACGTGGTGCGCAAACTGGGCTGGGGCCACTTCTCCACCGTCT
  GGCTCTGCTGGGACATCCAGCGCAAGCGCTTTGTGGCCCTCAAAGTGGTGAAGAGTGCGGGGCATTACAC
  GGAGACAGCTGTGGATGAGATCAAGCTCCTGAAATGTGTCCGGGACAGCGACCCCAGTGACCCCAAAAGA
  GAGACCATTGTCCAGCTCATTGATGACTTCAGGATCTCAGGAGTCAATGGAGTCCATGTGTGCATGGTGC
  TGGAGGTGCTGGGCCACCAGCTCCTCAAATGGATCATCAAGTCCAACTACCAGGGCCTGCCCGTGCCCTG
  CGTGAAGAGCATCGTGAGGCAGGTGCTGCACGGCCTGGACTACCTCCACACCAAGTGCAAGATCATCCAC
  ACGGACATCAAGCCCGAGAACATCTTGCTGTGTGTGGGGGACGCTTACATCAGGCGCCTGGCTGCCGAGG
  CCACGGAGTGGCAACAGGCAGGGGCGCCGCCCCCCTCCCGCTCCATAGTCAGCACTGCCCCCCAGGAGGT
  CTTGCAGACCGGTAAGCTGTCCAAAAACAAGAGGAAGAAGATGAGGCGCAAACGGAAACAGCAGAAGCGG
  CTGCTGGAGGAGCGGCTGCGGGACCTGCAGAGGCTGGAGGCCATGGAGGCTGCCACCCAGGCTGAGGACT
  CTGGCTTGAGACTAGACGGGGGCAGCGGCTCCACATCCTCTTCAGGCTGTCACCCCGGGGGCGCCAGAGC
  AGGTCCCTCCCCAGCCTCTTCCTCCCCCGCCCCGGGGGGCGGCCGTAGCCTCAGCGCGGGCTCACAGACC
  TCAGGCTTCTCCGGCTCCCTCTTCTCTCCTGCCTCCTGCTCCATCCTCTCCGGCTCGTCCAATCAGCGAG
  AGACCGGGGGCCTCCTGTCGCCTAGCACACCATTCGGTGCCTCGAACCTCCTGGTGAACCCCCTGGAGCC
  CCAAAATGCAGATAAGATCAAGATCAAGATCGCAGACCTGGGCAACGCCTGCTGGGTGCACAAGCACTTC
  ACGGAAGACATCCAGACTCGGCAGTACCGGGCCGTCGAGGTGCTGATCGGCGCCGAATACGGCCCCCCGG
  CAGACATCTGGAGCACAGCCTGCATGGCCTTCGAGCTGGCCACTGGTGACTACCTGTTCGAGCCGCATTC
  TGGAGAAGACTACAGTCGTGATGAGGACCACATCGCTCACATAGTGGAGCTTCTGGGGGACATCCCCCCA
  GCCTTCGCCCTCTCAGGCCGCTATTCCCGGGAGTTCTTCAACCGGAGAGGAGAGCTGCGGCACATCCACA
  ATCTCAAGCACTGGGGCCTGTACGAGGTACTCATGGAAAAGTACGAGTGGCCCCTAGAGCAGGCCACACA
  GTTCAGCGCCTTTCTGCTGCCCATGATGGAGTACATCCCCGAAAAGCGGGCCAGTGCCGCTGACTGCCTC
  CAGCACCCCTGGCTCAACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215496 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSASTGGGGDSGGSGGSSSSSQASCGPESSGSELALATPVPQMLQGLLGSDDEEQEDPKDYCKGGYHPVK
  IGDVFNGRYHVVRKLGWGHFSTVWLCWDIQRKRFVALKVVKSAGHYTETAVDEIKLLKCVRDSDPSDPKR
  ETIVQLIDDFRISGVNGVHVCMVLEVLGHQLLKWIIKSNYQGLPVPCVKSIVRQVLHGLDYLHTKCKIIH
  TDIKPENILLCVGDAYIRRLAAEATEWQQAGAPPPSRSIVSTAPQEVLQTGKLSKNKRKKMRRKRKQQKR
  LLEERLRDLQRLEAMEAATQAEDSGLRLDGGSGSTSSSGCHPGGARAGPSPASSSPAPGGGRSLSAGSQT
  SGFSGSLFSPASCSILSGSSNQRETGGLLSPSTPFGASNLLVNPLEPQNADKIKIKIADLGNACWVHKHF
  TEDIQTRQYRAVEVLIGAEYGPPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIVELLGDIPP
  AFALSGRYSREFFNRRGELRHIHNLKHWGLYEVLMEKYEWPLEQATQFSAFLLPMMEYIPEKRASAADCL
  QHPWLNP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014370
• ORF Size: 1701 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014370.2, NP_055185.2
• RefSeq Size: 2014 bp
• RefSeq ORF: 1704 bp
• Locus ID: 26576
• UniProt ID: Q9UPE1
• Cytogenetics: Xq28
• Protein Families: Druggable Genome, Protein Kinase
• MW: 62 kDa
• Gene Summary: This gene encodes a protein kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. A highly similar protein has been shown to play a role in muscle development in mice. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]